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Sisplatin’e Maruz Bırakılan Sıçan Böbreğinde Lutein’in Koruyucu Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

Yıl 2022, Cilt: 4 Sayı: 3, 433 - 438, 22.09.2022
https://doi.org/10.37990/medr.1142424

Öz

Amaç: Çalışmamızda, sisplatin (Cis) (10mg/kg, intraperitoneal) kaynaklı böbrek hasarına karşı lutein’in (L) (100mg/kg, oral) koruyucu etkisi incelendi.
Materyal ve Metot: Bu çalışma, 8 haftalık 28 adet sıçanla gerçekleştirildi. Oluşturulan 4 grubun her birinde 7 tane sıçan yer aldı: Kontrol, L, Cis ve Cis + L.
Bulgular: Biyokimyasal bulgulara göre, Cis grubunun kontrol grubu ile kıyaslanmasında serum Cr ve BUN seviyelerinin anlamlı düzeyde arttığı görüldü (p<0.05). Cis + L grubunda serum Cr ve BUN düzeylerinin Cis grubuna kıyasla anlamlı düzeyde azaldığı tespit edildi (p<0.05). Ayrıca Cis + L grubunun Cis grubu ile kıyaslanmasında MDA seviyesinde düşüş, GSH, SOD ve CAT seviyelerinde ise artış kaydedildi (p<0.05). Yapılan histopatolojik incelemelerde Cis grubu sıçanların böbrek dokularında interstisyel mononükleer lökosit infiltrasyonu, tubuler dejenerasyon ve tubuler nekroz gibi patolojik değişiklikler gözlendi. Cis + L grubu Cis grubu ile kıyaslandığında L’nin etkisi ile oksidatif streste bir azalma, antioksidan aktivitede bir artma ve histopatolojik değişikliklerde bir azalma olduğu söylenebilir.
Sonuç: Bu sonuçlar L’nin Cis kaynaklı böbrek hasarının önlenmesinde etkili olduğunu göstermektedir. Ayrıca L’nin bu hasarda kullanılma potansiyeli olan bir farmakolojik ajan olduğuda ortaya çıkmaktadır.

Destekleyen Kurum

Projemizi fonlayan herhangi bir kurum bulunmamaktadır.

Proje Numarası

2022/036

Kaynakça

  • 1. Yahyazadeh A. Morphometric alteration induced by cyclophosphamide in rat kidney and protective efficacy of coenzyme Q10: A stereological study. Medicine Science. 2021;10(4):1122-7.
  • 2. Bilgic S, Armagan I. Effects of misoprostol treatment on doxorubicin induced renal injury in rats. Biotechnic & Histochemistry. 2020;95(2):113-20.
  • 3. Bilgic S, Ozgocmen M. The protective effect of misoprostol against doxorubicin induced liver injury. Biotechnic & Histochemistry. 2019;94(8):583-91.
  • 4. Alhoshani AR, Hafez MM, Husain S, et al. Protective effect of rutin supplementation against cisplatin-induced nephrotoxicity in rats. BMC Nephrol. 2017;18(1):194-200.
  • 5. Morsy MA, Heeba GH. Nebivolol ameliorates cisplatin-induced nephrotoxicity in rats. Basic & Clinical Pharmacology & Toxicology. 2016;118(6):449-455.
  • 6. Abd El-Rhman RH, El-Naga RN, Gad AM, et al. Dibenzazepine attenuates against cisplatin-induced nephrotoxicity in rats: involvement of NOTCH pathway. Frontiers in Pharmacology. 2020;11:1-10.
  • 7. Badreldin HA, Al Moundhri MS. Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food Chem Toxicol. 2006;44:1173-1183.
  • 8. Badary OA, Abdel-Maksoud S, Ahmed WA, et al. Naringenin attenuates cisplatin nephrotoxicity in rats. Life Sci. 2005;76:2125-2135.
  • 9. Söğüt S, Kotuk M, Yilmaz HR, et al. In vivo evidence suggesting a role for purine-catabolizing enzymes in the pathogenesis of cisplatin-induced nephrotoxicity in rats and effect of erdosteine against this toxicity. Cell Biochem Funct. 2004;22:157-162.
  • 10. Pérez-Rojas JM, Cruz C, García-López P, et al. Renoprotection by a-mangostin is related to the attenuation in renal oxidative/nitrosative stress induced by cisplatin nephrotoxicity. Free Radical Res. 2009;43:1122-1132.
  • 11. Bilgic S, Özgöçmen M, Özer MK, et al. Misoprostol ameliorates doxorubicin induced cardiac damage by decreasing oxidative stress and apoptosis in rats. Biotechnic and Histochemistry. 2020;95(7):514-21.
  • 12. Bilgic S, Aktas I. Investigation of protective effects of misoprostol against paclitaxel-induced ovarian damage in rats. Annals of Medical Research. 2022;29(3):233-239.
  • 13. Bilgiç S, Taştemir Korkmaz D, Azirak S, et al. Risperidone-induced renal damage and metabolic side effects: The protective effect of resveratrol. Oxidative Medicine and Cellular Longevity. 2017;8709521:1-10.
  • 14. Ojima F, Sakamoto H, Ishiguro Y, et al. Consumption of carotenoids in photosensitized oxidation of human plasma and plasma low‑density lipoprotein. Free Radic Biol Med. 1993;15:377-384.
  • 15. Krishnaswamy R, Devaraj SN, Padma VV. Lutein protects HT‑29 cells against deoxynivalenol‑induced oxidative stress and apoptosis: prevention of NF‑kappa B nuclear localization and down regulation of NF‑kappa B and cyclo‑oxygenase‑2 expression. Free Radic Biol Med. 2010;49:50-60.
  • 16. Chucair AJ, Rotstein NP, Sangiovanni JP, et al. Lutein and zeaxanthin protect photoreceptors from apoptosis induced by oxidative stress: relation with docosahexaenoic acid. Invest Ophthalmol Vis Sci. 2007;48:5168-5177.
  • 17. Jávor T, Bata M, Lovász L, et al. Gastric cytoprotective effects of vitamin A and other carotenoids. Int J Tissue React. 1983;5:289-296.
  • 18. Mozsik G, Bódis B, Figler M, et al. Mechanisms of action of retinoids in gastrointestinal mucosal protection in animals, human healthy subjects and patients. Life Sci. 2001;69:103-112.
  • 19. Katyal T, Singh G, Budhiraja RD, et al. Effect of lutein in development of experimental diabetic nephropathy in rats. Afr J Pharm Pharmacol. 2013;7:2953-2959.
  • 20. Capasso G, Giordano DR, De Tommaso G, et al. Parathyroidectomy has a beneficial effect on experimental cisplatin nephrotoxicity. Clin Nephrol. 1990;33(4):184-191.
  • 21. Gur FM, Timurkaan S, Taskin E, et al. Thymoquinone improves testicular damage and sperm quality in experimentally varicocele-induced adolescent rats. Andrologia. 2021;53(5):e14033.
  • 22. Gür F, Aktaş İ. Silymarin protects kidneys from paclitaxel-induced nephrotoxicity. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(3):452-458.
  • 23. Balcioglu E, Gur FM, Gur HE, et al. Histological structure of nannospalax xanthodon cochlea tissue. Biologia 2021;76:2543-2548.
  • 24. Junge W, Wilke B, Halabi A, et al. Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffé method. Clin Chim Acta. 2004;344:137-148.
  • 25. Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
  • 26. Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in Enzymology. 1990;186:421-31.
  • 27. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-7.
  • 28. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974;47(3):469-74.
  • 29. Aebi H. Catalase in vitro. Methods in Enzymology. 1984;105:121-6.
  • 30. Sastry J, Kellie SJ. Severe neurotoxicity, ototoxicity and nephrotoxicity following high-dose cisplatin and amifostine. Pediatr Hematol Oncol. 2005;22(5):441-5.
  • 31. Korkmaz DT, Azirak S, Bilgiç S, et al. Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain. Annals of Medical Research. 2021;28(11):2005-2011.
  • 32. Kadikoylu G, Bolaman Z, Demir S, et al. The effects of desferrioxamine on cisplatin-induced lipid peroxidation and the activities of antioxidant enzymes in rat kidneys. Hum Exp Toxicol. 2004;23:29-34.
  • 33. Lau AH. Apoptosis induced by cisplatin nephrotoxic injury. Kidney Int. 1999;56(4):1295-1298.
  • 34. Sabuncuoğlu S, Özgüneş H. Sisplatin toksisitesi: Oksidatif stresin önemi ve antioksidanların etkisi. Tıp Fakültesi Dergisi. 2010;1:73:3.
  • 35. Nazari Soltan Ahmad S, Rashtchizadeh N, Argani H, et al. Tangeretin protects renal tubular epithelial cells against experimental cisplatin toxicity. Iran J Basic Med Sci. 2019;22:179-186.
  • 36. Bilgiç S, Dogan Z, Azirak S, et al. Hepatoprotective effect of royal jelly, grape seed extract, and Lycium barbarum against diethylnitrosamine-induced liver toxicity in rats. Annals of Medical Research. 2018;25(3):342-348. 37. Bilgiç S, Taştemir Korkmaz D, Azirak S, et al. Olanzapine-induced renal damages and metabolic side effects: the protective effects of thymoquinone. J Turgut Ozal Med Cent. 2018;25(1):70-75.
  • 38. Gür FM, Aktaş İ, Bilgiç S, et al. Misoprostol alleviates paclitaxel-induced liver damage through its antioxidant and anti-apoptotic effects. Mol Cell Toxicol. 2022;18:393-400.
  • 39. Ozer MK, Bilgic S, Armagan I, et al. Thymoquinone protection from amikacin induced renal injury in rats. Biotechnic & Histochemistry. 2020;95(2):129-136.
  • 40. Tanbek K, Ozerol E, Bilgic S, et al. Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science. 2017;26(1):96‐103.

Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin

Yıl 2022, Cilt: 4 Sayı: 3, 433 - 438, 22.09.2022
https://doi.org/10.37990/medr.1142424

Öz

Aim: The aim of this study was to determine the protection of lutein (L) (100 mg/kg, orally) against kidney damage caused by cisplatin (Cis) (10mg/kg, intraperitoneal).
Materials and Methods: Twenty-eight rats aged 8 weeks were used and divided into four equal groups (n=7): Control, L, Cis, and Cis + L.
Results: As a result of biochemical findings, serum Cr and BUN levels of the Cis group were significantly increased compared to the control group (p<0.05). Serum Cr and BUN levels of the Cis + L group were found to be significantly reduced compared to the Cis group (p<0.05). In addition, a decrease in MDA level and an increase in GSH, SOD and CAT levels were detected in the Cis + L group compared to the Cis group (p<0.05). In the histopathological examinations, in the Cis group kidney tissues; pathological changes such as interstitial mononuclear leukocyte infiltration, tubular degeneration, and tubular necrosis were observed. When the Cis + L group is compared with the Cis group, it can be said that there is a decrease in oxidative stress, an increase in antioxidant activity and a decrease in histopathological changes with the effect of L.
Conclusion: These results show that L is effective in preventing Cis-induced kidney injury. It is also emerging that L is a pharmacological agent with the potential to be used in this damage.

Proje Numarası

2022/036

Kaynakça

  • 1. Yahyazadeh A. Morphometric alteration induced by cyclophosphamide in rat kidney and protective efficacy of coenzyme Q10: A stereological study. Medicine Science. 2021;10(4):1122-7.
  • 2. Bilgic S, Armagan I. Effects of misoprostol treatment on doxorubicin induced renal injury in rats. Biotechnic & Histochemistry. 2020;95(2):113-20.
  • 3. Bilgic S, Ozgocmen M. The protective effect of misoprostol against doxorubicin induced liver injury. Biotechnic & Histochemistry. 2019;94(8):583-91.
  • 4. Alhoshani AR, Hafez MM, Husain S, et al. Protective effect of rutin supplementation against cisplatin-induced nephrotoxicity in rats. BMC Nephrol. 2017;18(1):194-200.
  • 5. Morsy MA, Heeba GH. Nebivolol ameliorates cisplatin-induced nephrotoxicity in rats. Basic & Clinical Pharmacology & Toxicology. 2016;118(6):449-455.
  • 6. Abd El-Rhman RH, El-Naga RN, Gad AM, et al. Dibenzazepine attenuates against cisplatin-induced nephrotoxicity in rats: involvement of NOTCH pathway. Frontiers in Pharmacology. 2020;11:1-10.
  • 7. Badreldin HA, Al Moundhri MS. Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food Chem Toxicol. 2006;44:1173-1183.
  • 8. Badary OA, Abdel-Maksoud S, Ahmed WA, et al. Naringenin attenuates cisplatin nephrotoxicity in rats. Life Sci. 2005;76:2125-2135.
  • 9. Söğüt S, Kotuk M, Yilmaz HR, et al. In vivo evidence suggesting a role for purine-catabolizing enzymes in the pathogenesis of cisplatin-induced nephrotoxicity in rats and effect of erdosteine against this toxicity. Cell Biochem Funct. 2004;22:157-162.
  • 10. Pérez-Rojas JM, Cruz C, García-López P, et al. Renoprotection by a-mangostin is related to the attenuation in renal oxidative/nitrosative stress induced by cisplatin nephrotoxicity. Free Radical Res. 2009;43:1122-1132.
  • 11. Bilgic S, Özgöçmen M, Özer MK, et al. Misoprostol ameliorates doxorubicin induced cardiac damage by decreasing oxidative stress and apoptosis in rats. Biotechnic and Histochemistry. 2020;95(7):514-21.
  • 12. Bilgic S, Aktas I. Investigation of protective effects of misoprostol against paclitaxel-induced ovarian damage in rats. Annals of Medical Research. 2022;29(3):233-239.
  • 13. Bilgiç S, Taştemir Korkmaz D, Azirak S, et al. Risperidone-induced renal damage and metabolic side effects: The protective effect of resveratrol. Oxidative Medicine and Cellular Longevity. 2017;8709521:1-10.
  • 14. Ojima F, Sakamoto H, Ishiguro Y, et al. Consumption of carotenoids in photosensitized oxidation of human plasma and plasma low‑density lipoprotein. Free Radic Biol Med. 1993;15:377-384.
  • 15. Krishnaswamy R, Devaraj SN, Padma VV. Lutein protects HT‑29 cells against deoxynivalenol‑induced oxidative stress and apoptosis: prevention of NF‑kappa B nuclear localization and down regulation of NF‑kappa B and cyclo‑oxygenase‑2 expression. Free Radic Biol Med. 2010;49:50-60.
  • 16. Chucair AJ, Rotstein NP, Sangiovanni JP, et al. Lutein and zeaxanthin protect photoreceptors from apoptosis induced by oxidative stress: relation with docosahexaenoic acid. Invest Ophthalmol Vis Sci. 2007;48:5168-5177.
  • 17. Jávor T, Bata M, Lovász L, et al. Gastric cytoprotective effects of vitamin A and other carotenoids. Int J Tissue React. 1983;5:289-296.
  • 18. Mozsik G, Bódis B, Figler M, et al. Mechanisms of action of retinoids in gastrointestinal mucosal protection in animals, human healthy subjects and patients. Life Sci. 2001;69:103-112.
  • 19. Katyal T, Singh G, Budhiraja RD, et al. Effect of lutein in development of experimental diabetic nephropathy in rats. Afr J Pharm Pharmacol. 2013;7:2953-2959.
  • 20. Capasso G, Giordano DR, De Tommaso G, et al. Parathyroidectomy has a beneficial effect on experimental cisplatin nephrotoxicity. Clin Nephrol. 1990;33(4):184-191.
  • 21. Gur FM, Timurkaan S, Taskin E, et al. Thymoquinone improves testicular damage and sperm quality in experimentally varicocele-induced adolescent rats. Andrologia. 2021;53(5):e14033.
  • 22. Gür F, Aktaş İ. Silymarin protects kidneys from paclitaxel-induced nephrotoxicity. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(3):452-458.
  • 23. Balcioglu E, Gur FM, Gur HE, et al. Histological structure of nannospalax xanthodon cochlea tissue. Biologia 2021;76:2543-2548.
  • 24. Junge W, Wilke B, Halabi A, et al. Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffé method. Clin Chim Acta. 2004;344:137-148.
  • 25. Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
  • 26. Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in Enzymology. 1990;186:421-31.
  • 27. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-7.
  • 28. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974;47(3):469-74.
  • 29. Aebi H. Catalase in vitro. Methods in Enzymology. 1984;105:121-6.
  • 30. Sastry J, Kellie SJ. Severe neurotoxicity, ototoxicity and nephrotoxicity following high-dose cisplatin and amifostine. Pediatr Hematol Oncol. 2005;22(5):441-5.
  • 31. Korkmaz DT, Azirak S, Bilgiç S, et al. Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain. Annals of Medical Research. 2021;28(11):2005-2011.
  • 32. Kadikoylu G, Bolaman Z, Demir S, et al. The effects of desferrioxamine on cisplatin-induced lipid peroxidation and the activities of antioxidant enzymes in rat kidneys. Hum Exp Toxicol. 2004;23:29-34.
  • 33. Lau AH. Apoptosis induced by cisplatin nephrotoxic injury. Kidney Int. 1999;56(4):1295-1298.
  • 34. Sabuncuoğlu S, Özgüneş H. Sisplatin toksisitesi: Oksidatif stresin önemi ve antioksidanların etkisi. Tıp Fakültesi Dergisi. 2010;1:73:3.
  • 35. Nazari Soltan Ahmad S, Rashtchizadeh N, Argani H, et al. Tangeretin protects renal tubular epithelial cells against experimental cisplatin toxicity. Iran J Basic Med Sci. 2019;22:179-186.
  • 36. Bilgiç S, Dogan Z, Azirak S, et al. Hepatoprotective effect of royal jelly, grape seed extract, and Lycium barbarum against diethylnitrosamine-induced liver toxicity in rats. Annals of Medical Research. 2018;25(3):342-348. 37. Bilgiç S, Taştemir Korkmaz D, Azirak S, et al. Olanzapine-induced renal damages and metabolic side effects: the protective effects of thymoquinone. J Turgut Ozal Med Cent. 2018;25(1):70-75.
  • 38. Gür FM, Aktaş İ, Bilgiç S, et al. Misoprostol alleviates paclitaxel-induced liver damage through its antioxidant and anti-apoptotic effects. Mol Cell Toxicol. 2022;18:393-400.
  • 39. Ozer MK, Bilgic S, Armagan I, et al. Thymoquinone protection from amikacin induced renal injury in rats. Biotechnic & Histochemistry. 2020;95(2):129-136.
  • 40. Tanbek K, Ozerol E, Bilgic S, et al. Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science. 2017;26(1):96‐103.
Toplam 39 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Özgün Makaleler
Yazarlar

Sedat Bilgiç 0000-0001-8410-2685

Fatih Mehmet Gür 0000-0001-7748-3272

İbrahim Aktaş 0000-0002-0956-8204

Proje Numarası 2022/036
Yayımlanma Tarihi 22 Eylül 2022
Kabul Tarihi 11 Ağustos 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 4 Sayı: 3

Kaynak Göster

AMA Bilgiç S, Gür FM, Aktaş İ. Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin. Med Records. Eylül 2022;4(3):433-438. doi:10.37990/medr.1142424

 Chief Editors

Assoc. Prof. Zülal Öner
Address: İzmir Bakırçay University, Department of Anatomy, İzmir, Turkey

Assoc. Prof. Deniz Şenol
Address: Düzce University, Department of Anatomy, Düzce, Turkey

E-mail: medrecsjournal@gmail.com

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