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Gossypin Regulated Doxorubicin-Induced Oxidative Stress and Inflammation in H9c2 Cardiomyocyte Cells

Yıl 2024, Cilt: 6 Sayı: 1, 44 - 49, 31.01.2024
https://doi.org/10.37990/medr.1383719

Öz

Aim: Doxorubicin (DOX), an anthracycline, is widely used in chemotherapy due to its effectiveness in fighting many cancers. Experimental and clinical studies prove that this drug damages non-targeted tissues (including cardiomyocytes) and reduces patients' quality of life during and after DOX treatment. The discovery of potent compounds as a protective tool to slow cardiomyocyte damage during the use of anti-cancer drugs such as DOX is crucial for both more effective cancer treatment and to improve patient's quality of life. Gossypin (GOS) is a flavonoid with several important properties, such as anti-cancer, analgesic, antioxidant, and anti-inflammatory. GOS shows supportive effects against oxidative stress and inflammation by activating antioxidant defense enzymes.
Material and Method: For the study, four groups were formed from H9c2 embryonic cardiomyocyte cells as Control, DOX (1 μM, 48 h), GOS25 (25 µg/ml, 48 h), and GOS50 (50 µg/ml, 48 h). In the study, Total antioxidant and oxidant status (TAS and TOS), levels of the inflammatory cytokines IL 1 beta and 6, and TNF α, lipid peroxidation levels as malondialdehyde (MDA), glutathione peroxidase (GSHPx), and glutathione (GSH) levels in the H9c2 embryonic cardiomyocyte cells were determined.
Results: The results showed that DOX treatment caused cell toxicity in the embryonic cardiomyocyte cells and increased TOS, IL 1 beta and 6, TNF α, and MDA levels while decreasing TAS, GSH, and GSHPx levels. This situation improved with GOS treatment.
Conclusion: As a result, it was determined that GOS treatment showed a protective effect in the DOX-induced cell toxicity model in H9c2 embryonic cardiomyocyte cell lines.

Etik Beyan

sunuldu

Kaynakça

  • Zhang J, Li W, Xue S, et al. Qishen granule attenuates doxorubicin-induced cardiotoxicity by protecting mitochondrial function and reducing oxidative stress through regulation of Sirtuin3. J Ethnopharmacol. 2023 Sep 13. doi: 10.1016/j.jep.2023.117134. [Epub ahead of print].
  • Yildizhan K, Huyut Z, Altındağ F, Uçar B. Effect of selenium and N-(P-Amylcinnamoyl) anthranilic acid on doxorubicin-induced kidney injury in rats. Journal of Inonu University Health Services Vocational School. 2023;11:1181-91.
  • Anker MS, Hadzibegovic S, Lena A, et al. Recent advances in cardio-oncology: a report from the ’heart failure association 2019 and world congress on acute heart failure 2019’. ESC Heart Fail. 2019;6:1140-8.
  • Catanzaro MP, Weiner A, Kaminaris A, et al. Doxorubicin-induced cardiomyocyte death is mediated by unchecked mitochondrial fission and mitophagy. FASEB J. 2019;33:11096-108.
  • Christidi E, Brunham LR. Regulated cell death pathways in doxorubicin-induced cardiotoxicity. Cell Death Dis. 2021;12:339.
  • Shaker RA, Abboud SH, Assad HC, Hadi N. Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. BMC Pharmacol Toxicol. 2018;19:3.
  • Yıldızhan K, Huyut Z, Altındağ F. Involvement of TRPM2 channel on doxorubicin-induced experimental cardiotoxicity model: protective role of selenium. Biol Trace Elem Res. 2023;201:2458-69.
  • Yazğan B, Yazğan Y, Nazıroğlu M. Alpha-lipoic acid modulates the diabetes mellitus-mediated neuropathic pain via inhibition of the TRPV1 channel, apoptosis, and oxidative stress in rats. J Bioenerg Biomembr. 2023;55:179-93.
  • Yazğan B, Yazğan Y. Regulatory role of phospholipase A2 inhibitor in oxidative stress and inflammation induced by an experimental mouse migraine model. J Cell Neurosci Oxid Stress. 2023;15:1147-56.
  • Koçkar MC, Nazıroğlu M, Celik O, et al. N-acetylcysteine modulates doxorubicin-induced oxidative stress and antioxidant vitamin concentrations in liver of rats. Cell Biochem Funct. 2010;28:673-7.
  • Hsieh DJ, Tsai BC, Barik P, et al. Human adipose-derived stem cells preconditioned with a novel herbal formulation Jing Shi attenuate doxorubicin-induced cardiac damage. Aging (Albany NY). 2023;15:9167-81.
  • Abd El-Aziz TA, Mohamed RH, Pasha HF, Abdel-Aziz HR. Catechin protects against oxidative stress and inflammatory-mediated cardiotoxicity in Adriamycin treated rats. Clin Exp Med. 2012;12:233-40.
  • Cinar I, Yayla M, Tavaci T, et al. In vivo and in vitro cardioprotective effect of gossypin against isoproterenol-induced myocardial infarction injury. Cardiovasc Toxicol. 2022;22:52-62.
  • Song B, Shen X, Tong C, et al. Gossypin: a flavonoid with diverse pharmacological effects. Chem Biol Drug Des. 2023;101:131-7.
  • Katary M, Salahuddin A. Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats. Life Sci. 2017;176:75-81.
  • Zhang Q, Li J, Peng S, et al. Rosmarinic acid as a candidate in a phenotypic profiling cardio-/cytotoxicity cell model induced by doxorubicin. Molecules. 2020;25:836.
  • Werida RH, Elshafiey RA, Ghoneim A, et al. Role of alpha-lipoic acid in counteracting paclitaxel- and doxorubicin-induced toxicities: a randomized controlled trial in breast cancer patients. Support Care Cancer. 2022;30:7281-92.
  • Cheng G, Zhang J, Jia S, et al. Cardioprotective effect of gossypin against myocardial ischemic/reperfusion in rats via alteration of oxidative stress, inflammation and gut microbiota. J Inflamm Res. 2022;15:1637-51.
  • Hu C, Zhang X, Song P, et al. Meteorin-like protein attenuates doxorubicin-induced cardiotoxicity via activating cAMP/PKA/SIRT1 pathway. Redox Biol. 2020;37:101747.
  • Kuznetsov AV, Javadov S, Sickinger S, et al. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta. 2015;1853:276-84.
  • Kanno SI, Hara A. Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT. Toxicol In Vitro. 2023;93:105698.
  • Yazğan Y, Nazıroğlu M. Involvement of TRPM2 in the neurobiology of experimental migraine: focus on oxidative stress and apoptosis. Mol Neurobiol. 2021;58:5581-601.
  • Yazğan B, Yazğan Y. Potent antioxidant alpha lipoic acid reduces STZ-induced oxidative stress and apoptosis levels in the erythrocytes and brain cells of diabetic rats. J Cell Neurosci Oxid Stress. 2022;14:1085-94.
  • Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem. 1966;16:359-64.
  • Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;24;25:192-205.
  • Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 1976;71:952-8.
  • Sheibani M, Azizi Y, Shayan M, et al. Doxorubicin-induced cardiotoxicity: an overview on pre-clinical therapeutic approaches. Cardiovasc Toxicol. 2022;22:292-310.
  • Saleh Ahmed AS. Potential protective effect of catechin on doxorubicin-induced cardiotoxicity in adult male albino rats. Toxicol Mech Methods. 2022;32:97-105.
  • Deng J, Huang M, Wu H. Protective efect of limonin against doxorubicin-induced cardiotoxicity via activating nuclear factor - like 2 and Sirtuin 2 signaling pathways. Bioengineered. 2021;12:7975-84.
  • Ekinci Akdemir FN, Yildirim S, Kandemir FM, et al. Protective efects of gallic acid on doxorubicin-induced cardiotoxicity; an experimantal study. Arch Physiol Biochem. 2021;127:258-65.
  • Broeyer FJ, Osanto S, Suzuki J, et al. Evaluation of lecithinized human recombinant super oxide dismutase as cardioprotectant in anthracycline-treated breast cancer patients. Br J Clin Pharmacol. 2014;78:950-60.
  • Sun Z, Yan B, YanYu W, et al. Vitexin attenuates acute doxorubicin cardiotoxicity in rats via the suppression of oxidative stress, inflammation and apoptosis and the activation of FOXO3a. Exp Ther Med. 2016;12:1879-84.
  • He Y, Yang Z, Li J, Li E. Dexmedetomidine reduces the inflammation and apoptosis of doxorubicin-induced myocardial cells. Exp Mol Pathol. 2020;113:104371.
  • Sun Z, Lu W, Lin N, et al. Dihydromyricetin alleviates doxorubicin-induced cardiotoxicity by inhibiting NLRP3 inflammasome through activation of SIRT1. Biochem Pharmacol. 2020;175:113888.
  • Çınar I. Evaluation of protective effects of gossypin against hydrogen peroxide damage in L929 fibroblast cells. Kafkas J Med Sci. 2020;10:15-23.
Yıl 2024, Cilt: 6 Sayı: 1, 44 - 49, 31.01.2024
https://doi.org/10.37990/medr.1383719

Öz

Kaynakça

  • Zhang J, Li W, Xue S, et al. Qishen granule attenuates doxorubicin-induced cardiotoxicity by protecting mitochondrial function and reducing oxidative stress through regulation of Sirtuin3. J Ethnopharmacol. 2023 Sep 13. doi: 10.1016/j.jep.2023.117134. [Epub ahead of print].
  • Yildizhan K, Huyut Z, Altındağ F, Uçar B. Effect of selenium and N-(P-Amylcinnamoyl) anthranilic acid on doxorubicin-induced kidney injury in rats. Journal of Inonu University Health Services Vocational School. 2023;11:1181-91.
  • Anker MS, Hadzibegovic S, Lena A, et al. Recent advances in cardio-oncology: a report from the ’heart failure association 2019 and world congress on acute heart failure 2019’. ESC Heart Fail. 2019;6:1140-8.
  • Catanzaro MP, Weiner A, Kaminaris A, et al. Doxorubicin-induced cardiomyocyte death is mediated by unchecked mitochondrial fission and mitophagy. FASEB J. 2019;33:11096-108.
  • Christidi E, Brunham LR. Regulated cell death pathways in doxorubicin-induced cardiotoxicity. Cell Death Dis. 2021;12:339.
  • Shaker RA, Abboud SH, Assad HC, Hadi N. Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. BMC Pharmacol Toxicol. 2018;19:3.
  • Yıldızhan K, Huyut Z, Altındağ F. Involvement of TRPM2 channel on doxorubicin-induced experimental cardiotoxicity model: protective role of selenium. Biol Trace Elem Res. 2023;201:2458-69.
  • Yazğan B, Yazğan Y, Nazıroğlu M. Alpha-lipoic acid modulates the diabetes mellitus-mediated neuropathic pain via inhibition of the TRPV1 channel, apoptosis, and oxidative stress in rats. J Bioenerg Biomembr. 2023;55:179-93.
  • Yazğan B, Yazğan Y. Regulatory role of phospholipase A2 inhibitor in oxidative stress and inflammation induced by an experimental mouse migraine model. J Cell Neurosci Oxid Stress. 2023;15:1147-56.
  • Koçkar MC, Nazıroğlu M, Celik O, et al. N-acetylcysteine modulates doxorubicin-induced oxidative stress and antioxidant vitamin concentrations in liver of rats. Cell Biochem Funct. 2010;28:673-7.
  • Hsieh DJ, Tsai BC, Barik P, et al. Human adipose-derived stem cells preconditioned with a novel herbal formulation Jing Shi attenuate doxorubicin-induced cardiac damage. Aging (Albany NY). 2023;15:9167-81.
  • Abd El-Aziz TA, Mohamed RH, Pasha HF, Abdel-Aziz HR. Catechin protects against oxidative stress and inflammatory-mediated cardiotoxicity in Adriamycin treated rats. Clin Exp Med. 2012;12:233-40.
  • Cinar I, Yayla M, Tavaci T, et al. In vivo and in vitro cardioprotective effect of gossypin against isoproterenol-induced myocardial infarction injury. Cardiovasc Toxicol. 2022;22:52-62.
  • Song B, Shen X, Tong C, et al. Gossypin: a flavonoid with diverse pharmacological effects. Chem Biol Drug Des. 2023;101:131-7.
  • Katary M, Salahuddin A. Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats. Life Sci. 2017;176:75-81.
  • Zhang Q, Li J, Peng S, et al. Rosmarinic acid as a candidate in a phenotypic profiling cardio-/cytotoxicity cell model induced by doxorubicin. Molecules. 2020;25:836.
  • Werida RH, Elshafiey RA, Ghoneim A, et al. Role of alpha-lipoic acid in counteracting paclitaxel- and doxorubicin-induced toxicities: a randomized controlled trial in breast cancer patients. Support Care Cancer. 2022;30:7281-92.
  • Cheng G, Zhang J, Jia S, et al. Cardioprotective effect of gossypin against myocardial ischemic/reperfusion in rats via alteration of oxidative stress, inflammation and gut microbiota. J Inflamm Res. 2022;15:1637-51.
  • Hu C, Zhang X, Song P, et al. Meteorin-like protein attenuates doxorubicin-induced cardiotoxicity via activating cAMP/PKA/SIRT1 pathway. Redox Biol. 2020;37:101747.
  • Kuznetsov AV, Javadov S, Sickinger S, et al. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta. 2015;1853:276-84.
  • Kanno SI, Hara A. Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT. Toxicol In Vitro. 2023;93:105698.
  • Yazğan Y, Nazıroğlu M. Involvement of TRPM2 in the neurobiology of experimental migraine: focus on oxidative stress and apoptosis. Mol Neurobiol. 2021;58:5581-601.
  • Yazğan B, Yazğan Y. Potent antioxidant alpha lipoic acid reduces STZ-induced oxidative stress and apoptosis levels in the erythrocytes and brain cells of diabetic rats. J Cell Neurosci Oxid Stress. 2022;14:1085-94.
  • Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem. 1966;16:359-64.
  • Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;24;25:192-205.
  • Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 1976;71:952-8.
  • Sheibani M, Azizi Y, Shayan M, et al. Doxorubicin-induced cardiotoxicity: an overview on pre-clinical therapeutic approaches. Cardiovasc Toxicol. 2022;22:292-310.
  • Saleh Ahmed AS. Potential protective effect of catechin on doxorubicin-induced cardiotoxicity in adult male albino rats. Toxicol Mech Methods. 2022;32:97-105.
  • Deng J, Huang M, Wu H. Protective efect of limonin against doxorubicin-induced cardiotoxicity via activating nuclear factor - like 2 and Sirtuin 2 signaling pathways. Bioengineered. 2021;12:7975-84.
  • Ekinci Akdemir FN, Yildirim S, Kandemir FM, et al. Protective efects of gallic acid on doxorubicin-induced cardiotoxicity; an experimantal study. Arch Physiol Biochem. 2021;127:258-65.
  • Broeyer FJ, Osanto S, Suzuki J, et al. Evaluation of lecithinized human recombinant super oxide dismutase as cardioprotectant in anthracycline-treated breast cancer patients. Br J Clin Pharmacol. 2014;78:950-60.
  • Sun Z, Yan B, YanYu W, et al. Vitexin attenuates acute doxorubicin cardiotoxicity in rats via the suppression of oxidative stress, inflammation and apoptosis and the activation of FOXO3a. Exp Ther Med. 2016;12:1879-84.
  • He Y, Yang Z, Li J, Li E. Dexmedetomidine reduces the inflammation and apoptosis of doxorubicin-induced myocardial cells. Exp Mol Pathol. 2020;113:104371.
  • Sun Z, Lu W, Lin N, et al. Dihydromyricetin alleviates doxorubicin-induced cardiotoxicity by inhibiting NLRP3 inflammasome through activation of SIRT1. Biochem Pharmacol. 2020;175:113888.
  • Çınar I. Evaluation of protective effects of gossypin against hydrogen peroxide damage in L929 fibroblast cells. Kafkas J Med Sci. 2020;10:15-23.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular İnsan Biyofiziği
Bölüm Özgün Makaleler
Yazarlar

Yener Yazğan 0000-0002-5613-6906

Betül Yazğan 0000-0002-4029-2007

Yayımlanma Tarihi 31 Ocak 2024
Gönderilme Tarihi 31 Ekim 2023
Kabul Tarihi 6 Aralık 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 6 Sayı: 1

Kaynak Göster

AMA Yazğan Y, Yazğan B. Gossypin Regulated Doxorubicin-Induced Oxidative Stress and Inflammation in H9c2 Cardiomyocyte Cells. Med Records. Ocak 2024;6(1):44-49. doi:10.37990/medr.1383719

 Chief Editors

Assoc. Prof. Zülal Öner
Address: İzmir Bakırçay University, Department of Anatomy, İzmir, Turkey

Assoc. Prof. Deniz Şenol
Address: Düzce University, Department of Anatomy, Düzce, Turkey

E-mail: medrecsjournal@gmail.com

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