Azerbaycan Propolislerinin Antioksidan Potansiyeli ve Fenolik Profili

Yıl 2015, Cilt: 15 Sayı: 1, 16 - 28, 31.05.2016

Zehra Can Oktay Yıldız , Hüseyin Şahin Alsever Asadov Sevgi Kolaylı

Öz

Çalışmada Azerbaycan’ın 15 farklı bölgesinden toplanan propolis örneklerinin fenolik kompozisyonu ve antioksidan özellikleri analiz edildi. Antioksidan aktiviteleri üç farklı metod kullanılarak belirlendi; I) toplam fenolik içerik, ii) Demir indirgeme antioksidan kapasite (FRAP testi), iii) DPPH radikali süpürme aktivitesi. Propolis örneklerinin toplam fenolik içeriği 10.94–79.23mgGAE/g arasında, FRAP değerleri 170–438µM Trolox/g arasında, DPPH radikal süpürme aktivitesi (SC50 değeri) 18–128µg/mL arasında bulundu.Fenolik asitler ve flavonoidler HPLC-DAD kullanılarak, standartlarla karşılaştırılarak tanımlandı. Araştırılan 15 örnekten, Ismayilli, Zerdap ve Qax bölgesinden toplanan propolislerin kapasitesi diğer bölgelerde toplananlardan yüksek bulundu. Örneklerin toplam antioksidan kapasiteleri propolislerin toplandığı bölgenin florasına bağlı olarak değişmektedir ancak bununla birlikte aynı bölge içerisin de toplanan örnekler arasında da anlamlı farklılıklar tespit edildi. Çalışma bulguları propolis örneklerinin antioksidan potansiyelinin sadece bölgenin florasına bağlı olmadığını, kovanın konumuna, kovanda bulunan propolisin ne kadar süredir kovanda biriktiğine, koloninin gücüne ve numunelerin toplanma yeri ve şekline de bağlı olduğunu gösterdi.

Anahtar Kelimeler

Propolis, antioksidan aktivite, fenolik bileşikler, FRAP, DPPH

Phenolic Profile and Antioxidant Potential of Propolis from Azerbaijan

Öz

This study analyzed the antioxidant properties and phenolic compositions of propolis samples collected from 15 different locations in Azerbaijan. Antioxidant activities were measured using three different methods, i) total phenolic contents (TPC), ii) ferric reducing/antioxidant power (FRAP) and iii) 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity. TPC of the propolis samples were between 10.94 and 79.23 mg GAE/g, FRAP values were between 170 and 438 µM Trolox/g and DPPH radical scavenging activities (SC50 value) were between 18 and 128 µg/mL. Phenolic acids and flavonoids were identified using HPLC-DAD in comparison with standards. Of the 15 samples investigated, propolis samples from the Ismayilli, Zerdap and Qax regions exhibited higher antioxidant capacity compared to the other regions of Azerbaijan. The total antioxidant potential of the samples varied depending on the flora of the different collection regions; however, there were also significant differences among samples collected within the same region. The study findings reveal that the antioxidant potential of propolis samples does not depend only on the flora of the region, but that the condition and age of the beehive, as well as the strength of the colony and method of collection of the samples are also important dynamics.

Anahtar Kelimeler

Propolis, antioxidant activity, phenolic compounds, FRAP, DPPH

Kaynakça

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