in vitro Bakteriyel Test Sistemleri Kullanılarak Propolisin Genotoksisite ve Antigenotoksisitesinin Değerlendirilmesi

Yıl 2015, Cilt: 15 Sayı: 1, 29 - 36, 31.05.2016

Nesrin Ecem Bayram Mehmet Karadayı , Medine Güllüce Sinan Bayram , Kadriye Sorkun Gül Cevahir Öz Mehmet Nuri Aydoğan Taha Yasin Koç Burak Alaylar Bekir Salih

Öz

Propolis (arı tutkalı) yüzyıllardır terapötik bir ajan olarak yaygın bir şekilde kullanılmaktadır. Farmakolojik ve biyolojik özelliklerinden dolayı bu konu üzerindeki bilimsel ilgi son yıllarda giderek artmaktadır. Bu çalışmanın amacı, propolisin ethanol ekstraktının (EEP) kimyasal komposizyonunu belirlemek ve ayrıca Ames/Salmonella ve E. coli WP2 test sistemlerini kullanarak propolisin genotoksik ve antigenotoksik etkilerini değerlendirmektir. Canlılık testlerinden elde edilen sonuçlar, EEP’nin 1mg/plate ve daha yüksek konsantrasyonlarının oldukça toksik olduğunu, daha düşük konsantrasyonların ise toksik etkisinin olmadığını göstermiştir. Genotoksisite test sonuçları ise, EEP’nin 1 mg/plate ve daha düşük konsantrasyonlarda, S. typhimurium TA1535, 1537 ve E. coli WP2uvrA test suşları için genotoksik etkisinin olmadığını göstermiştir. Ayrıca, aynı test suşları için mutajen etkiye sahip NaN3 , 9-AA and MNNG gibi mutajenlere karşı EEP’nin, belirgin bir şekilde antigenotoksik etkiye sahip olduğu gözlemlenmiştir.

Anahtar Kelimeler

Propolis, GC-MS, Genotoksisite, Antigenotoksisite, Ames testi

Genotoxic and Antigenotoxic Evaluation of Propolis by Using in vitro Bacterial Assay Systems

Öz

Propolis (bee glue) has been widely used as a therapeutic agent for centuries. Because of its pharmacological and biological properties, scientific interest in this issue has increased during the last years. The aims of the study were to determine chemical composition of ethanol extract of propolis (EEP) and evaluate its genotoxicity and antigenotoxicity by using Ames/Salmonella and E. coli WP2 short term test systems. Results that obtained from the viability assay for the tester strains, EEP at concentrations of greater than 1 mg/plate was highly toxic however lower concentration doesn’t have any toxicity. According to genotoxicity test results, any concentration of EEP up to 1 mg/plate did not show genotoxic effect on S. typhimurium TA1535, 1537 and E. coli WP2uvrA tester strains. On the other hand, antigenotoxicity assay results showed that EEP has significantly antigenotoxic activity against NaN3 , 9-AA and MNNG induced mutagenicity on the same tester strains.

Anahtar Kelimeler

Propolis, GC-MS, Genotoxicity, Antigenotoxicity, Ames test.

Kaynakça

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