Araştırma Makalesi
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Evaluation of Nurses’ Knowledge Levels on Blood Culture Collection

Yıl 2025, Cilt: 1 Sayı: 3, 189 - 203, 25.09.2025

Öz

ABSTRACT
Objective: This study aimed to determine nurses’ knowledge levels regarding blood culture collection.
Materials and Methods: A cross-sectional study was conducted with 249 nurses at a training and research hospital between July and September 2022. Data were collected using a personal information form and a knowledge questionnaire on blood culture collection.
Results: The mean age of the participants was 32.42±6.46 years, and 76.3% were female. The correct response rates to the questions regarding blood culture collection knowledge ranged from 15.7% to 94.4%. The findings revealed that nurses’ knowledge levels were significantly associated with gender, educational status, length of professional experience, unit of employment, and prior training on blood culture collection (p<0.05). It was determined that male nurses, those with higher levels of education and professional experience, those working in clinical units, and those who had received training on blood culture collection had higher correct response rates.
Conclusion: The study revealed that nurses have adequate knowledge about set contents and culture bottles but show significant gaps in critical areas such as optimal blood volume, inoculation amount, key points during culture collection, and causes of contamination. The findings indicate a need for systematic and targeted training programs on blood culture collection.

Kaynakça

  • Van Daalen FV, Smeulers M, Bartels EJH, Holleman F, Visser CE, Geerlings SE. A healthcare failure mode and effect analysis to optimise the process of blood culture performance. Neth J Med. 2020; 78(6):341-348. PMID: 33380531
  • Hemeg HA, Almutairi AZ, Alharbi NL, Alenezi RF, Alturkostani MA, Ozbak HA, Islam FA. Blood culture contamination in a tertiary care hospital of Saudi Arabia: A one-year study. Saudi Med J. 2020; 41(5): 508-515. https://doi.org/10.15537/smj.2020.5.25052
  • Klimud. Kan dolaşımı örneklerinin laboratuvar incelemesi rehberi. 2022. https://www.klimud.org/icerik/rehberler-38/klimud-rehberleri-8265 (Erişim tarihi: 01.07.2025)
  • Doern GV, Carroll KC, Diekema DJ, Garey KW, Rupp ME, Weinstein MP, Sexton DJ. Practical guidance for clinical microbiology laboratories: A comprehensive update on the problem of blood culture contamination and a discussion of methods for addressing the problem. Clin Microbiol Rev. 2019; 33(1): e00009-19. https://doi.org/10.1128/CMR.00009-19
  • Panday RSN, Wang S, Van De Ven PM, Hekker TAM, Alam N, Nanayakkara PWB. Evaluation of blood culture epidemiology and efficiency in a large European teaching hospital. PLoS One. 2019; 14(3): e0214052. https://doi.org/10.1371/journal.pone.0214052
  • Mokhtar NA, Ting SY, Zainol Abidin NZ, et al. Blood culture contamination rates and common microorganisms in Malaysian public hospitals: A multicenter retrospective study. Malays J Pathol. 2024;46(2):307-314.
  • Rahden P, Barrow E, Bah H, Bittaye SO, Nygren D, Badjan A. Bloodstream infections at a tertiary hospital in the Gambia: A one-year retrospective study. BMC Infect Dis. 2025; 25(1): 1-9. https://doi.org/10.1186/s12879-025-10533-1
  • Ramirez Galleymore P, Gordón Sahuquillo M. Antisepsis for blood culture extraction: Blood culture contamination rate. Med Intensiva. 2019; 43: 31-34. https://doi.org/10.1016/j.medin.2018.08.007
  • Geisler BP, Jilg N, Patton RG, Pietzsch JB. Model to evaluate the impact of hospital-based interventions targeting false-positive blood cultures on economic and clinical outcomes. Journal of Hospital Infection. 2019; 102(4):438-444. https://doi.org/10.1016/j.jhin.2019.03.012
  • Sezgin FM, Babaoglu UT. Blood culture results at a research and training hospital and the importance of training. Niger J Clin Pract. 2019; 22(12):1693-1697. https://doi.org/10.4103/njcp.njcp_573_18
  • Nair A, Elliott SP, Al Mohajer M. Knowledge, attitude, and practice of blood culture contamination: A multicenter study. Am J Infect Control. 2017; 45(5):547-548. https://doi.org/10.1016/j.ajic.2017.01.008
  • Corboy JB, Attridge M. Impact of Blood Culture Contamination on Patients and Health Care Systems: A Review of QI Strategies Within the ED. Clin Pediatr Emerg Med. 2019; 20(3):100718. https://doi.org/10.1016/j.cpem.2019.100718
  • Ruiz JS, Frota OP, Ferreira Júnior MA. Knowledge, Attitudes, and Practices of nurses regarding blood culture collection. Rev Bras Enferm; 2025; 77(6):e20230424. https://doi.org/10.1590/0034-7167-2023-0424
  • Öztik S, Çavdar M, Karakoyun A, Özmen Ünal H. Hemşirelerin Kan Kültürü Örneği Alımına İlişkin Uygulama Basamaklarının Değerlendirilmesi. THDD. 2025; 6(1):1-10. https://doi.org/10.70479/thdd.1646733
  • Raupach-Rosin H, Duddeck A, Gehrlich M, Helmke C, Huebner J, Pletz MW, Mikolajczyk R, Karch A. Deficits in knowledge, attitude, and practice towards blood culture sampling: results of a nationwide mixed-methods study among inpatient care physicians in Germany. Infection. 2017; 45(4): 433-441. https://doi.org/10.1007/s15010-017-0990-7
  • Hughes JA, Cabilan CJ, Williams J, Ray M, Coyer F. The effectiveness of interventions to reduce peripheral blood culture contamination in acute care: a systematic review protocol. Syst Rev. 2018; 7(1):216. https://doi.org/10.1186/s13643-018-0877-4
  • Kumthekar I, Urs T, Rajashekar D, Karthik K. Effectiveness of Multimodal Intervention to Improve Blood Culture Collection in a Tertiary Care Hospital. Cureus. 2024; 16(2):e53941. https://doi.org/10.7759/cureus.53941
  • Romann L, Werlen L, Rommers N, et al. Factors impacting the pre-analytical quality of blood cultures-Analysis at a tertiary medical center. PLoS One. 2023; 18(3):e0282918. https://doi.org/10.1371/journal.pone.0282918
  • Romem A, Rozani V. Gender-related differences in the scope of nursing practice: Evidence from a cross-sectional study in geriatric healthcare settings. BMC Nursing. 2024; 23, 852. https://doi.org/10.1186/s12912-024-02516-5
  • Shayan Z, Khoddami N, Jafari P, Askarian M, Borazjani R. Gender differences in nurses' knowledge, practice, and attitudes towards contact isolation precautions: A measurement invariance study. Shiraz E-Medical Journal. 2025; 26(3): e148167. https://doi.org/10.5812/semj-148167

Hemşirelerin Kan Kültürü Alımı Hakkındaki Bilgi Düzeylerinin Belirlenmesi

Yıl 2025, Cilt: 1 Sayı: 3, 189 - 203, 25.09.2025

Öz

ÖZET
Amaç: Bu çalışmada, hemşirelerin kan kültürü alımı hakkında bilgi düzeylerinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntemler: Kesitsel tipteki araştırma, Temmuz-Eylül 2022 tarihleri arasında bir eğitim ve araştırma hastanesinde 249 hemşire ile yürütülmüştür. Veriler, kişisel bilgi formu ve kan kültürü alımına yönelik bilgi düzeyi anketi ile toplanmıştır.
Bulgular: Katılımcıların yaş ortalaması 32,42±6,46 olup, %76,3’ü kadındır. Kan kültürü alımına ilişkin bilgi düzeyi sorularına verilen doğru yanıt oranları %15,7 ile %94,4 arasında değişmektedir. Bulgular, hemşirelerin bilgi düzeylerinin cinsiyet, eğitim düzeyi, mesleki deneyim süresi, çalışma birimi ve kan kültürü eğitimi alma durumu ile anlamlı biçimde ilişkili olduğunu göstermiştir (p<0,05). Erkek hemşirelerin, eğitim ve mesleki deneyim düzeyi yüksek olanların, klinik birimlerde görev yapanların ve kan kültürü eğitimi almış olanların doğru yanıt oranlarının daha yüksek olduğu saptanmıştır.
Sonuç: Çalışma, hemşirelerin set içeriği ve kültür şişeleri konusunda yeterli bilgiye sahip olduklarını; ancak optimal kan hacmi, inokülasyon miktarı, kültür alımında dikkat edilecek noktalar ve kontaminasyon nedenleri gibi kritik alanlarda bilgi eksiklikleri bulunduğunu ortaya koymuştur. Bulgular, kan kültürü alımı için sistematik ve hedeflenmiş eğitim programlarına ihtiyaç olduğunu göstermektedir.

Teşekkür

Çalışmada hiçbir kurum ya da kişiden finansal destek alınmamıştır. Araştırmaya katılan tüm araştırmacılara teşekkür ederiz.

Kaynakça

  • Van Daalen FV, Smeulers M, Bartels EJH, Holleman F, Visser CE, Geerlings SE. A healthcare failure mode and effect analysis to optimise the process of blood culture performance. Neth J Med. 2020; 78(6):341-348. PMID: 33380531
  • Hemeg HA, Almutairi AZ, Alharbi NL, Alenezi RF, Alturkostani MA, Ozbak HA, Islam FA. Blood culture contamination in a tertiary care hospital of Saudi Arabia: A one-year study. Saudi Med J. 2020; 41(5): 508-515. https://doi.org/10.15537/smj.2020.5.25052
  • Klimud. Kan dolaşımı örneklerinin laboratuvar incelemesi rehberi. 2022. https://www.klimud.org/icerik/rehberler-38/klimud-rehberleri-8265 (Erişim tarihi: 01.07.2025)
  • Doern GV, Carroll KC, Diekema DJ, Garey KW, Rupp ME, Weinstein MP, Sexton DJ. Practical guidance for clinical microbiology laboratories: A comprehensive update on the problem of blood culture contamination and a discussion of methods for addressing the problem. Clin Microbiol Rev. 2019; 33(1): e00009-19. https://doi.org/10.1128/CMR.00009-19
  • Panday RSN, Wang S, Van De Ven PM, Hekker TAM, Alam N, Nanayakkara PWB. Evaluation of blood culture epidemiology and efficiency in a large European teaching hospital. PLoS One. 2019; 14(3): e0214052. https://doi.org/10.1371/journal.pone.0214052
  • Mokhtar NA, Ting SY, Zainol Abidin NZ, et al. Blood culture contamination rates and common microorganisms in Malaysian public hospitals: A multicenter retrospective study. Malays J Pathol. 2024;46(2):307-314.
  • Rahden P, Barrow E, Bah H, Bittaye SO, Nygren D, Badjan A. Bloodstream infections at a tertiary hospital in the Gambia: A one-year retrospective study. BMC Infect Dis. 2025; 25(1): 1-9. https://doi.org/10.1186/s12879-025-10533-1
  • Ramirez Galleymore P, Gordón Sahuquillo M. Antisepsis for blood culture extraction: Blood culture contamination rate. Med Intensiva. 2019; 43: 31-34. https://doi.org/10.1016/j.medin.2018.08.007
  • Geisler BP, Jilg N, Patton RG, Pietzsch JB. Model to evaluate the impact of hospital-based interventions targeting false-positive blood cultures on economic and clinical outcomes. Journal of Hospital Infection. 2019; 102(4):438-444. https://doi.org/10.1016/j.jhin.2019.03.012
  • Sezgin FM, Babaoglu UT. Blood culture results at a research and training hospital and the importance of training. Niger J Clin Pract. 2019; 22(12):1693-1697. https://doi.org/10.4103/njcp.njcp_573_18
  • Nair A, Elliott SP, Al Mohajer M. Knowledge, attitude, and practice of blood culture contamination: A multicenter study. Am J Infect Control. 2017; 45(5):547-548. https://doi.org/10.1016/j.ajic.2017.01.008
  • Corboy JB, Attridge M. Impact of Blood Culture Contamination on Patients and Health Care Systems: A Review of QI Strategies Within the ED. Clin Pediatr Emerg Med. 2019; 20(3):100718. https://doi.org/10.1016/j.cpem.2019.100718
  • Ruiz JS, Frota OP, Ferreira Júnior MA. Knowledge, Attitudes, and Practices of nurses regarding blood culture collection. Rev Bras Enferm; 2025; 77(6):e20230424. https://doi.org/10.1590/0034-7167-2023-0424
  • Öztik S, Çavdar M, Karakoyun A, Özmen Ünal H. Hemşirelerin Kan Kültürü Örneği Alımına İlişkin Uygulama Basamaklarının Değerlendirilmesi. THDD. 2025; 6(1):1-10. https://doi.org/10.70479/thdd.1646733
  • Raupach-Rosin H, Duddeck A, Gehrlich M, Helmke C, Huebner J, Pletz MW, Mikolajczyk R, Karch A. Deficits in knowledge, attitude, and practice towards blood culture sampling: results of a nationwide mixed-methods study among inpatient care physicians in Germany. Infection. 2017; 45(4): 433-441. https://doi.org/10.1007/s15010-017-0990-7
  • Hughes JA, Cabilan CJ, Williams J, Ray M, Coyer F. The effectiveness of interventions to reduce peripheral blood culture contamination in acute care: a systematic review protocol. Syst Rev. 2018; 7(1):216. https://doi.org/10.1186/s13643-018-0877-4
  • Kumthekar I, Urs T, Rajashekar D, Karthik K. Effectiveness of Multimodal Intervention to Improve Blood Culture Collection in a Tertiary Care Hospital. Cureus. 2024; 16(2):e53941. https://doi.org/10.7759/cureus.53941
  • Romann L, Werlen L, Rommers N, et al. Factors impacting the pre-analytical quality of blood cultures-Analysis at a tertiary medical center. PLoS One. 2023; 18(3):e0282918. https://doi.org/10.1371/journal.pone.0282918
  • Romem A, Rozani V. Gender-related differences in the scope of nursing practice: Evidence from a cross-sectional study in geriatric healthcare settings. BMC Nursing. 2024; 23, 852. https://doi.org/10.1186/s12912-024-02516-5
  • Shayan Z, Khoddami N, Jafari P, Askarian M, Borazjani R. Gender differences in nurses' knowledge, practice, and attitudes towards contact isolation precautions: A measurement invariance study. Shiraz E-Medical Journal. 2025; 26(3): e148167. https://doi.org/10.5812/semj-148167
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Hemşirelik (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Betülay Kılıç 0000-0002-4178-4003

Betül Bilmen Ünal 0009-0007-6590-8887

Fatma İlknur Çinar 0000-0001-6394-8331

Yayımlanma Tarihi 25 Eylül 2025
Gönderilme Tarihi 17 Ağustos 2025
Kabul Tarihi 21 Ağustos 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 1 Sayı: 3

Kaynak Göster

APA Kılıç, B., Bilmen Ünal, B., & Çinar, F. İ. (2025). Hemşirelerin Kan Kültürü Alımı Hakkındaki Bilgi Düzeylerinin Belirlenmesi. Munzur Sağlık Bilimleri Dergisi, 1(3), 189-203.