Potential Effects of EGFR Exon 21 L858R Mutations in Lung Cancer

Yıl 2019, , 12 - 17, 28.02.2019

Ebru Derici Eker , Ali Arıcan , Rabia Bozdoğan Arpacı Erdinç Nayır Alper Ata Nazan Eras Didem Derici Yıldırım , Etem Akbaş

https://doi.org/10.17546/msd.493329

Öz

Objective: The mainly
significant reason in the etiology of lung cancer is smoking, which is
important in other environmental pollutants and genetic susceptibility. Lung
cancer is separated two major groups as mainly non-small cell and small cell
according to the growth rate, spread, timing of metastasis, response to
chemotherapy and radiotherapy. Epidermal growth factor receptor (EGFR)
constitutes the highest rate with 50-80% in gene mutations which are prognostic
value in lung cancer. Many studies have shown that EGFR is overexpressed in
lung cancer. In our study, we aimed to investigate the relationship between
EGFR gene exon 21 L858R mutations in lung cancer.

Material and Methods: Our sample
consisted of a healthy group of 190 healthy volunteers with the same age and
gender characteristics as the patient group of 178 patients who were diagnosed
as lung cancer in the Mersin University Medical Faculty Oncology clinic. The
DNAs were obtained according to the standard salt precipitation method.
Mutation detection and genotyping analyzes were identified by polymerase chain
reaction (PCR) and restriction fragment length polymorphism (RFLP) analyzes.

Results: Smoking was one
of the other risk factors for lung cancer, 130 (68.33%) in the control group
and 156 (87.5%) in the lung cancer group. 27 person of the lung cancer (15%)
were female and 151 (85%) were male. In the control group, 92 people (48.33%)
were in the wild genotype and 98 persons were in the mutant genotype (51.66%).
In the lung cancer group 80 (45%) were wild-type and 98 (55%) were mutant
genotypes. According to the histopathological types of lung cancer, EGFR-21
mutation heterozygous or homozygous carriers are proportionally compatible (p =
0.90).

Conclusion: According to
our findings, the EGFR-21 mutation is not associated with histopathological
types of lung cancer.

Anahtar Kelimeler

Lung cancer, EGFR gene, exon 21

Kaynakça

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