Türkiye’den Toplanan Bazı Yabani Mantarların Antimikrobiyal ve Anti-Quorum Sensing (Çoğunluğu Algılama) Aktiviteleri

Öz

Yabani mantarlar, tıbbi özellikleri nedeniyle çok eski tarihten beri bilinen, ormanların önemli bir parçasıdır. Bu çalışmada, Karadeniz bölgesinden toplanan bazı yabani mantarların (Amanita rubescens, Russula delica, Lactarius sp.) anti mikrobiyal ve anti-quorum sensing (çoğunluğu algılama) aktiviteleri incelenmiştir. Mantar ekstraktları süper kritik akışkan ekstraksiyonu metodu ile hazırlanmıştır. Ekstraktların antimikrobiyal potansiyelleri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 7002, Listeria monocytogenes ATCC 43251 and Candida albicans ATCC 10231 mikroorganizmalarına karşı agar kuyucuk difüzyon yöntemiyle test edilmiştir. Anti-quorum sensing (çoğunluğu algılama) aktivite ise Chromobacterium violaceum ATCC 12472 bakterisi üzerinde test edilmiştir. Sonuçlar, Amanita rubescens mantar ekstraktının Staphylococcus aureus'u inhibe ettiğini, Amanita rubescens ve Lactarius ssp. ekstraktlarının Chromobacterium violaceum a karşı anti-quorum sensing (çoğunluğu algılama) aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler

• Antimikrobiyal,Anti-Quorum Sensing (Çoğunluğu Algılama),Mantar,Türkiye

Antimicrobial and Anti-Quorum Sensing Activity of Some Wild Mushrooms Collected from Turkey

Öz

Wild mushrooms are an important part of forests that have been known since the early history for their excellent medicinal properties. In this study, anti-microbial and anti-quorum sensing activities of some wild mushrooms (Amanita rubescens, Russula delica, Lactarius sp.) collected from the Black Sea region in Turkey were investigated. Mushroom extracts were prepared by supercritical fluid extraction method. Antimicrobial potential of extracts was tested by agar well diffusion method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 7002, Listeria monocytogenes ATCC 43251 and Candida albicans ATCC 10231. Anti-quorum sensing activity was tested on Chromobacterium violaceum ATCC 12472 bacteria.  The results revealed that Amanita rubescens showed an inhibitory effect against Staphylococcus aureus and Amanita rubescens and Lactarius sp. extracts showed anti-quorum sensing activity against Chromobacterium violaceum.

Anahtar Kelimeler

• Anti-microbial,Anti-Quorum Sensing,Mushroom,Turkey

Kaynakça

1. Rasmussen T.B., Givskov M. Quorum sensing inhibitors: a bargain of effects, Microbiology 152 (4), 895-904, 2006.
2. Antunes L.C.M., Ferreira R.B., Buckner M.M., Finlay B.B. Quorum sensing in bacterial virulence, Microbiology, 156 (8), 2271-2282, 2010.
3. Agrahar-Murugkar D., Subbulakshmi G. Nutritional value of edible wild mushrooms collected from the Khasi hills of Meghalaya, Food Chemistry, 89 (4), 599-603, 2005.
4. Cheung L., Cheung P.C., Ooi V.E. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry, 81 (2), 249-255, 2003.
5. Choi Y., Lee S., Chun J., Lee H,. Lee, J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom, Food Chemistry, 99 (2), 381-387, 2006.
6. Barros L., Calhelha R.C., Vaz J.A., Ferreira I.C., Baptista P., Estevinho L.M. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts, European Food Research and Technology, 225 (2), 151-156, 2007.
7. Soković M., Griensven L.J. Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus, European Journal of Plant Pathology, 116 (3), 211-224, 2006.
8. Kim S.H., Song Y.S., Kim S.K., Kim B.C., Lim C.J., Park E.H. Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus, Journal of Ethnopharmacology, 93 (1), 141-146, 2004.

9. Jose N., Ajith T.A., Janardhanan K.K. Antioxidant, anti-inflammatory, and antitumor activities of culinary-medicinal mushroom Pleurotus pufmonanus (Fr.) Quel.(Agaricomycetideae), International Journal of Medicinal Mushrooms, 4 (4), 7 pages, 2002.
10. Chung M.J., Chung C.K., Jeong Y., Ham S.S. Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells. Nutrition Research and Practice, 4 (3), 177-182, 2010.
11. Park B.T., Na K.H., Jung E.C., Park J.W., Kim H.H. Antifungal and anticancer activities of a protein from the mushroom Cordyceps militaris, The Korean Journal of Physiology & Pharmacology, 13 (1), 49-54, 2009.
12. Ehssan H., Saadabi A. Screening of antimicrobial activity of wild mushrooms from Khartoum State of Sudan, Microbiol J, 2 (2), 64-69, 2012.
13. Alves M.J., Ferreira I.C., Martins A., Pintado M. Antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics, Journal of Applied Microbiology, 113 (2), 466-475, 2012.
14. Akyuz M., Onganer A.N., Erecevit P., Kirbag S. Antimicrobial activity of some edible mushrooms in the eastern and southeast Anatolia region of Turkey, Gazi University Journal of Science, 23 (2), 125-130, 2010.
15. Nowacka N., Nowak R., Drozd M., Olech M., Los R., Malm A. Antibacterial, antiradical potential and phenolic compounds of thirty-one Polish mushrooms, Plos One, 10 (10), e0140355, 2015.
16. Kosanic M., Rankovic B., Dasic M. Antioxidant and antimicrobial properties of mushrooms, Bulgarian Journal of Agricultural Science, 19 (5), 1040-1046, 2013.
17. Williams P. Quorum sensing, communication and cross-kingdom signalling in the bacterial world. Microbiology, 153 (12), 3923-3938, 2007.
18. Petrović J., Glamočlija J., Stojković D., Nikolić M., Ćirić A., Fernandes A., Ferreira I.C., Soković M. Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing- regulated functions and biofilm formation by Pseudomonas aeruginosa. Food & Function, 5 (12), 3296-3303, 2014.
19. Soković M., Ćirić A., Glamočlija J., Nikolić M., Griensven L.J. Agaricus blazei hot water extract shows anti quorum sensing activity in the nosocomial human pathogen Pseudomonas aeruginosa. Molecules, 19 (4), 4189-4199, 2014.