Rekürren aftöz stomatitli hastalarda serum ve tükürükte dermcidin düzeylerinin araştırılması ve tükürük bezinde dermcidin analizi

Öz

Amaç Rekürren aftöz stomatit (RAS) dudak mukozası, yanak ve dilde, tekrarlayıcı, küçük, ağrılı, eritemli halesi bulunan, nekrotik ülserlerle karakterize, kendi kendini sınırlayabilen, sık görülen bir oral mukoza hastalığıdır. Bu çalışmada RAS’lı hastaların serum ve tükürüğünde dermcidin düzeyinin, tükürük bezinde dermcidin varlığının ve RAS patogenezindeki rolünün belirlenmesi amaçlandı. Yöntem Bu çalışma 31 hasta ve 30 sağlıklı gönüllü ile yapıldı. Hasta ve kontrol grubunun serum ve tükürüğündeki dermcidin düzeyleri, insan dermcidin ELISA kitinin kataloglarında belirtilen çalışma prosedürlerine göre çalışıldı. Tükürük bezlerinde dermcidin varlığı immünohistokimyasal analiz ile değerlendirildi. Bulgular RAS’lı hasta grubunun ortalama tükürük dermcidin düzeyleri (105.80 ± 80.14), kontrol grubunun ortalama tükürük dermcidin düzeyleri (456.13 ± 354.59) ile kıyaslandığında istatistiksel anlamlı farklılık tespit edildi (P=0.000). RAS’lı hasta grubunda ortalama serum dermcidin düzeyleri (316.41 ± 784.55), kontrol grubunun ortalama serum dermcidin düzeyleri (130.65 ± 179.75) ile kıyaslandığında istatistiksel anlamlı farklılık tespit edilemedi. İmmünohistokimyasal boyamada parotis ve submandibular bezlerde, interlobular kanallarda dermcidin immünreaktivitesi gözlendi. Sonuç Sonuç olarak bu çalışmada tükürük bezinin striated hücrelerinin dermcidin sentezlediği ortaya kondu. Tükürükte antimikrobial özellikli dermcidin azlığının RAS için predispozan bir faktör olduğu düşünüldü.

Anahtar Kelimeler

• dermcidin
• rekürren aftöz stomatit
• tükürük bezi

Investigation of serum and saliva dermcidin levels in patients with recurrent aphthous stomatitis and dermcidin analysis in salivary gland

Öz

Objective Recurrent aphthous stomatitis (RAS) is a common self-limiting oral mucosa disease. In this study, it was aimed to determine the dermcidin level in the serum and saliva of patients with RAS, the presence of dermcidin in the salivary gland and its role in the pathogenesis of RAS. Methods Thirty-one patients presenting with RAS and 30 control subjects participated in this study. Dermcidin levels in serum and saliva of patients and control group were studied in accordance with the working procedures specified in the catalogs of the human dermcidin ELISA kit. The presence of dermcidin in salivary glands was assessed by immunohistochemical analysis. Results A statistically significant difference was found when the mean salivary dermcidin levels (105.80 ± 80.14 ng/mL) of the RAS patients were compared with the mean salivary dermcidin levels (456.13 ± 354.59 ng/mL) of the control group (P=0.000). There was no statistically significant difference between the mean serum dermcidin levels (316.41 ± 784.55 ng/mL) of the RAS patient and those of (130.65 ± 179.75 ng/mL) the control group. Dermcidin immunoreactivity was observed in the parotid gland, submandibular gland and interlobular striated ducts. Conclusion The findings in this study showed that striated cells in salivary gland synthesized dermcidin. Low levels of dermcidin with antimicrobial properties in saliva were considered as a predisposing factor for RAS.

Anahtar Kelimeler

• dermcidin
• salivary gland
• recurrent aphthous stomatitis

Kaynakça

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