INVESTIGATION OF PEROXIDASE-MIMICKING PROPERTIES OF FeNiCo METAL-ORGANIC FRAMEWORK DECORATED MWCNT COMPOSITE NANOMATERIAL

Öz

Enzyme-mimicking materials replicate natural enzyme functions, offering more durable, cost-effective, and eco-friendly alternatives, making them important for biotechnology and industrial applications. In this study, the peroxidase like properties of a FeNiCo metal-organic framework-decorated multi-walled carbon nanotube (FeNiCo MOF-MWCNT) composite nanomaterial were investigated for the first time. The nanocomposite was synthesized and characterized using Scanning Electron Microscopy (SEM), SEM-Mapping, SEM-EDS, XRD, and FT-IR techniques. Its enzyme-mimicking activity was evaluated using a colorimetric, non-enzymatic H₂O₂ biosensor with 3,3',5,5'-Tetramethylbenzidine (TMB) as the substrate. The biosensor comprises optimized amounts of FeNiCo MOF-MWCNT and TMB. Upon H₂O₂ addition, the nanocomposite mimics peroxidase activity by reducing H₂O₂ to H₂O, while oxidizing TMB to its blue-colored form, TMB(ox), showing absorbance at 654 nm. After optimizing conditions, the biosensor exhibited a linear response to H₂O₂ concentrations ranging from 0.1 mM to 12 mM. It also demonstrated high selectivity, with negligible interference from substances like KCl, ascorbic acid, urea, and NaCl. Accuracy tests using commercial H₂O₂ disinfectant showed results between 100% and 103%, confirming the biosensor's reliability for practical applications.

Anahtar Kelimeler

• Metal organic frameworks
• enzyme mimicking
• non-enzymatic H2O2 biosensor
• colorimetric biosensor

FeNiCo METAL-ORGANİK ÇERÇEVE İLE MODİFİYE EDİLMİŞ MWCNT KOMPOZİT NANOMALZEMENİN PEROKSİDAZ ENZİM TAKLİT ÖZELLİKLERİNİN İNCELENMESİ

Öz

Enzim taklit eden malzemeler, doğal enzimlerin işlevlerini taklit ederek daha dayanıklı, ekonomik ve çevre dostu alternatifler sunar; bu da biyoteknoloji ve endüstriyel uygulamalar için oldukça önemlidir. Bu çalışmada, FeNiCo metal-organik çerçeve modifiye çok duvarlı karbon nanotüp (FeNiCo MOF-MWCNT) kompozit nanomalzemenin, peroksidaz enzim benzeri özellikleri ilk kez incelenmiştir. Nanokompozit, Taramalı Elektron Mikroskobu (SEM), SEM Haritalama, SEM-EDS, X-Işını Difraksiyonu (XRD) ve Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR) teknikleriyle karakterize edilmiştir. FeNiCo MOF-MWCNT’nin enzim taklit özelliği, substrat olarak 3,3',5,5'-Tetrametilbenzidin (TMB) kullanılan enzim içermeyen kolorimetrik bir H₂O₂ biyosensörü ile incelenmiştir. Biyosensör çözeltisi, optimize edilmiş miktarda FeNiCo MOF-MWCNT ve TMB içermektedir. Çözeltiye H₂O₂ eklendiğinde, nanokompozit H₂O₂’yi suya indirgerken peroksidaz enzim aktivitesini taklit eder ve aynı anda TMB’yi oksitler, mavi renkli TMB(ox) formuna dönüşür; bu form 654 nm’de absorbsiyon gösterir. Deneysel koşullar optimize edildikten sonra biyosensör, 0.1 mM ile 12 mM arasındaki H₂O₂ derişimlerinde doğrusal yanıt vermiştir. Geliştirilen enzimsiz biyosensör üzerinde, KCl, askorbik asit, üre ve NaCl gibi girişimci maddelerden kaynaklı anlamlı bir etki gözlemlenmemiştir. Ticari H₂O₂ dezenfektanlarıyla yapılan doğruluk testlerinde sonuçlar %100–103 arasında bulunmuştur.

Anahtar Kelimeler

• Metal organik çerçeveler
• enzim taklit
• enzimsiz H2O2 biyosensörü
• kolorimetrik biyosensör

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