ÇEŞİTLİ MİKROBİYAL YAKIT HÜCRESİ TASARIMLARINDA METİLEN MAVİSİ ARACISI KULLANILARAK SACCHAROMYCES CEREVISIAE TARAFINDAN BİYOELEKTRİK ÜRETİMİ

Öz

Elektroaktif mikroorganizmaların ve elektron transfer mekanizmalarının keşfi, biyoelektrokimyasal atıksu arıtımı için yeni olanaklar açmıştır. Mikrobiyal yakıt hücreleri (MFC'ler), mikrobiyal metabolizma yoluyla organik substratları dönüştürerek elektrik üretir. Bu çalışmada, Saccharomyces cerevisiae, anot substratı olarak evsel atıksu kullanan çeşitli MFC tasarımlarının performansını değerlendirmek için biyokatalizör olarak kullanılmıştır. Reaktör konfigürasyonunun ve metilen mavisinin redoks aracısı olarak eklenmesinin voltaj üretimi, güç yoğunluğu ve kimyasal oksijen ihtiyacı (KOİ) giderimi üzerindeki etkileri araştırılmıştır. İki kesikli mod reaktör test edilmiştir: iç içe geçmiş silindirik çift odacıklı MFC (NDMFC) ve silindirik H tipi MFC (CHMFC). Elde edilen maksimum güç yoğunlukları NDMFC için 3,22 mW/m² ve CHMFC için 3,35 mW/m² iken, KOİ giderim verimlilikleri sırasıyla %90,33 ve %91,44 olmuştur. Tüm çalışma koşulları sabit tutulduğundan, gözlemlenen performans değişimleri reaktör tasarımındaki farklılıklara bağlanmıştır. Genel olarak, bu çalışma mikrobiyal yakıt hücrelerinde elektron transferini kolaylaştıran aracıların önemini vurgulamayı amaçlamaktadır. Metilen mavisinin güç üretimi üzerindeki etkisi, farklı reaktör konfigürasyonları kullanılarak etkili bir biçimde incelenmiştir.

Anahtar Kelimeler

• Saccharomyces cerevisiae
• Medyatör
• Güç yoğunluğu
• Mikrobiyal yakıt hücresi
• Biyoteknoloji

BIOELECTRICITY GENERATION BY SACCHAROMYCES CEREVISIAE USING METHYLENE BLUE MEDIATOR IN VARIOUS MICROBIAL FUEL CELL DESIGNS

Öz

The discovery of electroactive microorganisms and their electron transfer mechanisms has opened new possibilities for bioelectrochemical wastewater treatment. Microbial fuel cells (MFCs) generate electricity by converting organic substrates through microbial metabolism. In this study, Saccharomyces cerevisiae was utilized as a biocatalyst to assess the performance of various MFC designs using domestic wastewater as the anode substrate. The effects of reactor configuration and the addition of methylene blue as a redox mediator on voltage generation, power density, and chemical oxygen demand (COD) removal were investigated. Two batch-mode reactors were tested: a nested cylindrical dual-chamber MFC (NDMFC) and a cylindrical H-type MFC (CHMFC). The maximum power densities achieved were 3.22 mW/m² for the NDMFC and 3.35 mW/m² for the CHMFC, while COD removal efficiencies were 90.33% and 91.44%, respectively. As all operational conditions were maintained constant, the observed performance variations were attributed to differences in reactor design. Overall, this study aimed to emphasize the importance of mediators that facilitate electron transfer in microbial fuel cells. The effect of methylene blue on power generation was effectively investigated using different reactor configurations.

Anahtar Kelimeler

• Saccharomyces cerevisiae
• Mediator
• Power density
• Microbial fuel cell
• Biotechnology

Kaynakça

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