THE EFFECT OF GIBBERELLIC ACID (GA3) ON POST-GERMINATION MORPHOMETRIC PARAMETERS OF LISIANTHUS [Eustoma grandiflorum (Raf.) Shinn.] ‘MARIACHI PURE WHITE (F1)’ CULTIVAR

Öz

Lisianthus [Eustoma grandiflorum (Raf.) Shinn.], a member of the family Gentianaceae, is native to the central and southern part of United States. Lisianthus is a relatively new floral crop to the international market, quickly rankes in the top ten cut flowers worldwide due to its garish flowers and excellent post-harvest life. Due to its importance there are several tissue culture studies working on the micropropagation of this plant by using a variety of plant growth regulators (PGRs). With the aim of contribution to these works in the present study we aimed to investigate the effect of Gibberellic acid (GA₃) at different concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 mg l^-1) on morphometric parameters of Lisianthus during 30 and 80-day of incubation periods. In sum, our findings revealed that according to the results, there was no significant change for shoot number, shoot length and root number but very slight increase in number of leaves (1.0 mg l^-1) and root length (0.5 mg l^-1) of GA₃ during 30-day incubation period. On the other hand, for 80-day incubation period, the highest root length and number of leaves were obtained (respectively, 2.48 ± 1.25 cm and 14.17 ± 4.59) for 2.0 mg l^-1 concentration. In addition, shoot length to be found at the highest rate for 1.0 mg l^-1 (3.32 ± 0.34 cm). 80-day incubation period was found to be more effective than in comparison to 30-day incubation period in terms of number of leaves, shoot and root length.

Anahtar Kelimeler

• Eustoma grandiflorum,Lisianthus,Mariachi Pure White (F1),Gibberellic acid (GA3),In vitro

LISIANTHUS [EUSTOMA GRANDIFLORUM (RAF.) SHINN.] ‘MARIACHI PURE WHITE (F1)’ KÜLTIVARININ ÇIMLENME SONRASI MORFOMETRIK PARAMETRELERI ÜZERINDE GIBERELLIK ASIT (GA3)’IN ETKISI

Öz

Lisianthus [Eustoma grandiflorum (Raf.) Shinn.], Gentianaceae familyasının bir üyesi olup Orta ve Güney Amerika’nın yerel bitkisidir. Hasat sonrası kaliteli ömrü olması ve gösterişli çiçeklere sahip olmasından dolayı, dünya çapındaki uluslararası pazarda oldukça yeni bir çiçekli ürün olarak, kesme çiçek sektöründe hızlı bir şekilde ilk on sıralamasına girmiştir. Sahip olduğu öneminden dolayı bu bitkinin mikroçoğaltımı üzerinde çeşitli bitki büyüme düzenleyicileri (BBDler) kullanılarak yapılan birçok doku kültürü çalışması mevcuttur. Yapılan bu çalışmalara katkı sağlamak amacı ile 30 ve 80 günlük inkübasyon periyodu süresince farklı konsantrasyonlardaki (0.0, 0.5, 1.0, 2.0 and 4.0 mg l^-1) Giberellik asit (GA₃)’in Lisianthus’un morfometrik parametreleri üzerindeki etkisini araştırmak amaçlanmıştır. Özetle, verilen sonuçlara göre bulgularımız, 30 günlük inkübasyon periyodu süresince GA3’in sürgün sayısı, sürgün uzunluğu ve kök sayısı üzerinde önemli bir değişikliğe neden olmadığını fakat yaprak sayısı (1.0 mg l-1) ve kök uzunluğu (0.5 mg l^-1) parametreleri üzerinde nispeten artış gösterdiğini açıklığa kavuşturmuştur. Diğer bir taraftan 80 günlük inkübasyon periyodu süresince, en yüksek sürgün uzunluğu ve yaprak sayısı sırasıyla 2.48 ± 1.25 cm ve 14.17 ± 4.59 olarak 2.0 mg l^-1 konsantrasyondan elde edilmiştir. Buna ek olarak, en yüksek sürgün uzunluğu 1.0 mg l^-1 konsantrasyonda (3.32 ± 0.34 cm) bulunmuştur. Yaprak sayısı, sürgün uzunluğu ve kök uzunluğu açısından 30 günlük inkübasyon periyodu ile karşılaştırıldığında 80 günlük inkübasyon periyodu daha etkili bulunmuştur.

Anahtar Kelimeler

• Eustoma grandiflorum,Lisianthus,Mariachi Pure White (F1),Giberellik asit (GA3),In vitro

Kaynakça

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