Primary and Collagen Supported Scaffold Healing in Rabbit Horizontal Meniscus Ripped Models

Öz

The aim of this study is to investigate the potential for improvement of the collagen scaffold in the repair of horizontal meniscus tears in the rabbit model. Mature 21 New Zealand white male rabbits weighing 2.5-3 kg were divided into 3 equal groups, with 7 animals in each group. The medial meniscus was anteriorly horizontal torn from the horn to the middle. In accordance with the study protocol, Group 1 (n=7); only the horizontal meniscus was tilted in the control group. Group 2 (n=7); horizontal meniscus rupture primer repair. Group 3; horizontal meniscus were repaired with a colloidal scaffold. All experimental animals were sacrificed at the end of the 12th week. 1/3 anterior area of medial meniscus was measured. Histological analysis of the tissues was performed with Ishida score consisting of three components. Surface area of meniscus in group I was significantly lower than group II (p<0.008) and group III (p<0.002). The quality of the group III was significantly higher than the other groups (p<0.001). In the tears of the avascular region, such as the horizontal meniscus tears, scaffolds and similar materials can provide better healing and meniscal function and function close to normal anatomic tissue.

Anahtar Kelimeler

• Collagen Scaffold,Horizontal Rupture,Menuscus Repair,Primary Repair,Rabbit

Tavşanlarda Horizontal Menisküs Yırtık Modellerinde Primer ve Kollajen Skafold ile İyileşme

Öz

Bu çalışmanın amacı, tavşan modelinde horizontal menisküs yırtıklarının onarımında kollajen skafold kullanımı sonrası menisküsün iyileşme potansiyelini araştırmak ve skafold ile tamirin primer tamirden daha üstün olup olmadığını göstermektir. Ağırlıkları 2.5-3 kg arasında değişen olgun 21 adet New Zealand White cinsi erkek tavşanlar her grupta 7 hayvan olacak şekilde 3 eşit gruba ayrıldı. Medial menisküs anterior boynuzdan orta kısmına kadar horizontal yırtık modeli yapıldı. Çalışma protokolüne uygun olarak, Grup 1 (n=7); kontrol grubu sadece horizontal menisküs yırtığı olan tavşanlardan oluşturuldu. Grup 2 (n=7); horizontal menisküs yırtığa primer tamir yapılırken, Grup 3; horizontal menisküs yırtığa kollajen skafold ile tamir yapılanlar idi. Tüm deney hayvanları 12. haftanın sonunda sakrifiye edildi. Medial menisküsün 1/3 anterior alanı ölçüldü. Dokuların histolojik analizi, üç bileşenden oluşan Ishida skoru ile gerçekleştirildi. Grup I'deki menisküsün yüzey alanı grup II’den (p<0.008) ve grup III'ten (p<0.002) anlamlı olarak daha düşüktü. Grup III’deki dokunun kalitesi İshida puanına göre değerlendirildiğinde diğer gruplardan anlamlı olarak daha yüksekti (p<0.001). Horizontal menisküs yırtıkları gibi avasküler bölgelerindeki yırtıklarda skafold ve benzeri materyaller ile daha iyi iyileşme ve normal anatomik dokuya yakın işlev ve fonksiyonda menisküs elde edilebilir.

Anahtar Kelimeler

• Horizontal Yırtık,Kollajen Skafold,Menisküs Tamiri,Primer Tamir,Tavşan

Kaynakça

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