IntraUterineInsemination with Micro Fluid Sperm Sorting Chip Method: Case Report

Öz

World Health Organization (WHO) infertility; a couple's unprotected sexual inter course for one year. 15% of couples of reproductive age have infertility. Nowadays, the first step assisted reproduction technique is intra uterine insemination (IUI). This method allows the sperm and egg to meet in a controlled manner and with this method, sperm washing process and the catheter allows the determination of sperm into the uterus. Sperm washing techniques are gradient, flotation and micro fluidic chip method. The main principle of sperm washing is to collect the best quality sperm in the fastest and most gentle way. The main purpose of this type of sperm selection, no DNA fragmentation, have a nucleus, motile, is to provide a morphologically normal sperm. In this case, we aimed to investigate the utility of micro fluidic chip technique in insemination process.

Anahtar Kelimeler

• Infertility
• Insemination
• Sperm

Mikro Akışkan Sperm Ayıklama Chip Yöntemi ile İntraUterinİnseminasyon: Olgu Sunumu

Öz

Dünya Sağlık Örgütü (WHO) infertiliteyi; bir çiftin bir yıl boyunca düzenli korunmasız cinsel ilişkiye girdiği halde gebe kalamama durumu olarak tanımlamaktadır. İnfertilite üreme çağındaki çiftlerin yaklaşık %15 inde gözlenmektedir. Günümüzde ilk basamak yardımla üreme tekniği intrauterininseminasyon (IUI) yöntemidir. Bu yöntem kontrollü olarak spontan ya da medikal ajanlarla gerekli olgunluğa getirilen kadın üreme hücresi ile uyumlu olarak erkek üreme hücresi olan ve gerekli yıkama işlemlerinden geçirilen spermin bir kateter yardımı ile kontrollü olarak buluşmasını amaçlamaktadır. Sperm yıkama teknikleri dansitegradiyent, yüzdürme ve son dönemde mikro akışkan chip yöntemidir. Sperm yıkamada ana prensip en iyi kalitedeki spermi en hızlı ve en nazik şekilde toplayabilmektir. Bu tür sperm seçiminin ana amacı, DNA fragmantasyonu olmayan çekirdeğe sahip, hareketli, morfolojik olarak normal bir sperm sağlamaktır. Biz bu olgu ile yeni bir teknik olan Mikro akışkan sperm ayıklama chip yönteminin intrauterininseminasyon işleminde kullanılabilirliğini incelemeyi amaçladık.

Anahtar Kelimeler

• İnfertilite
• İnseminasyon
• Sperm

Kaynakça

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