IntraUterineInsemination with Micro Fluid Sperm Sorting Chip Method: Case Report

Yıl 2021, Cilt: 8 Sayı: 2, 141 - 144, 31.08.2021

Nazlı Karagöz Can , Sezen Bozkurt Köseoğlu

https://doi.org/10.47572/muskutd.617217

Öz

World Health Organization (WHO) infertility; a couple's unprotected sexual inter course for one year. 15% of couples of reproductive age have infertility. Nowadays, the first step assisted reproduction technique is intra uterine insemination (IUI). This method allows the sperm and egg to meet in a controlled manner and with this method, sperm washing process and the catheter allows the determination of sperm into the uterus. Sperm washing techniques are gradient, flotation and micro fluidic chip method. The main principle of sperm washing is to collect the best quality sperm in the fastest and most gentle way. The main purpose of this type of sperm selection, no DNA fragmentation, have a nucleus, motile, is to provide a morphologically normal sperm. In this case, we aimed to investigate the utility of micro fluidic chip technique in insemination process.

Anahtar Kelimeler

Infertility, Insemination, Sperm

Mikro Akışkan Sperm Ayıklama Chip Yöntemi ile İntraUterinİnseminasyon: Olgu Sunumu

Öz

Dünya Sağlık Örgütü (WHO) infertiliteyi; bir çiftin bir yıl boyunca düzenli korunmasız cinsel ilişkiye girdiği halde gebe kalamama durumu olarak tanımlamaktadır. İnfertilite üreme çağındaki çiftlerin yaklaşık %15 inde gözlenmektedir. Günümüzde ilk basamak yardımla üreme tekniği intrauterininseminasyon (IUI) yöntemidir. Bu yöntem kontrollü olarak spontan ya da medikal ajanlarla gerekli olgunluğa getirilen kadın üreme hücresi ile uyumlu olarak erkek üreme hücresi olan ve gerekli yıkama işlemlerinden geçirilen spermin bir kateter yardımı ile kontrollü olarak buluşmasını amaçlamaktadır. Sperm yıkama teknikleri dansitegradiyent, yüzdürme ve son dönemde mikro akışkan chip yöntemidir. Sperm yıkamada ana prensip en iyi kalitedeki spermi en hızlı ve en nazik şekilde toplayabilmektir. Bu tür sperm seçiminin ana amacı, DNA fragmantasyonu olmayan çekirdeğe sahip, hareketli, morfolojik olarak normal bir sperm sağlamaktır. Biz bu olgu ile yeni bir teknik olan Mikro akışkan sperm ayıklama chip yönteminin intrauterininseminasyon işleminde kullanılabilirliğini incelemeyi amaçladık.

Anahtar Kelimeler

İnfertilite, İnseminasyon, Sperm

Kaynakça

• 1. Subedi S, Lamichhane S, Chhetry M. Study of Infertile Couples Attending a Teaching Hospital in Eastern Nepal. JNMA J Nepal Med Assoc. 2016;55(203):22-5.
• 2. Inhorn MC, Patrizio P. Infertility around the globe: new thinking on gender, reproductive Technologies and global movements in the 21st century. Hum Reprod Update. 2015; 21:411-26.
• 3. World Health Organization: Laboratory manual for the examination and processing of human semen and Sperm Cervikal Mucus İnteraction, 5th ed. Cambridge: Cambridge Uni. WHO Press, 2010.
• 4. Speroff L, Fritz MA. Clinical Gynaecologic Endocrinology and Infertility. 8nd edition. 2011;978-1005.
• 5. Palermo GD, O’Neill CL, Chow S, et al. The Ronald O. Perelman and Claudia Cohen Intracytoplasmic sperm injection: state of the art in humans. Reproduction. 2017; 154(6):93-110.
• 6. Çokan Dönmez, Ç, Güner Emül, T. İnfertilite Tedavisinde Ortaya Çıkan Komplikasyonlar ve Hemşirelik Yaklaşımları. J Soc Human Sci Res. 2019; 6(43): 2958-66.
• 7. Abbas RH, Abbas SH. İntra uterıne İnsemınatıon (IUI) Pregnancy Rate After Hysterosalpıngo graphy (Hsg) World. J Pharma Res. 2018: 7(18):185-91.
• 8. Asghar W, Velasco V, Kingsley JL, et al. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater. 2014;3(10): 1671-79.
• 9. Cho CL, Agarwal A, Majzoub A, Esteves SC. A single cut-off value of sperm dna fragmentation testing does not fit all. Transl Androl Urol 2017; 6:501-3.
• 10. Nagata B, Endo K, Ogata K, et al. Live births from artificial insemination of micro fluidic sorted bovine spermatozoa characterized by trajectories correlated with fertility. PNAS. 2018;115(14):3087-96.
• 11. Brown SG, Publicover SJ, Barratt CLR, Silva SJ Human sperm ion channel (dys) function: implications for fertilization. Hum Reprod. 2019; 25(6): 758–76.
• 12. Barroso G, Valdespin C, Vega E, et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril. 2009;92(3):835-48.
• 13. Loutradi KE, Tarlatzis BC, Goulis DG, et al. The effects of sperm quality on embryo development after intra cytoplasmic sperm injection. J Assist Reprod Genet. 2006;23(2):69–74.
• 14. Henkel RR, Schill WB. Sperm preparationfor ART. Reprod Biol Endocrinol. 2003;1: 108-29.
• 15. Sugihara A, Van Avermaete F, Roelant E, Punjabi U, De Neubourg D.The role of sperm DNA fragmentation testing in predicting intra-uterine insemination outcome: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2020;244:8-15.
• 16. Magdanz V, Boryshpolets S, Ridzewski C, Eckel B, Reinhardt K. The motility-based swim-up technique separates bull sperm based on differences in metabolic rates and tail length. PLoS One. 2019:10;14(10).
• 17. Zini A, Finelli A, Phang D, Jarvi K. Influence of semen processing technique on human sperm dna integrity. Urology. 2000; 56: 1081-4.
• 18. Stiavnická M, Abril-Parreño L, Nevoral J, Kralickova M, García-Álvarez O. Non-Invasive Approaches to Epigenetic-Base Sperm Selection. Med Sci Monit. 2017; 23: 4677-83.
• 19. Quinn MM, Jalalian L, Ribeiro S, et al. Micro fluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod. 2018; 33: 1388-93.
• 20. Kissell KA, Danaher MR, Schisterman EF, et al. Biological variability in serum anti-Mullerian hormone throughout the menstrual cycle in ovulatory and sporadic an ovulatory cycles in eumenorrheic women. Hum Reprod. 2014;29(8):1764-72.
• 21. Kedem A, Haas J, Geva LL, et al. Ongoing pregnancy rates in women with low and extremely low AMH levels. A multivariate analysis of 769 cycles. PLoS One. 2013;8(12):81629-92.
• 22. Reichman DE, Goldschlag D, Rosenwaks Z. Value of antimullerian hormone as a prognostic indicator of in vitro fertilization outcome. Fertil Steril. 2014;101(4):1012-8.
• 23. Practice Committee of the American Society for Reproductive M. Testing and interpreting measures of ovarian reserve: a committee opinion. Fertil Steril. 2015;103(3):9-17.
• 24. La Marca A, Broekmans FJ, Volpe A, Fauser BC, Macklon NS. Anti-Mullerian hormone (AMH): what do we stil need to know? Hum Reprod. 2009;24(9):2264-75.
• 25. Gezginç K, Selimoğlu R, Yazıcı F. Erken Membran Rüptürüne Güncel Yaklaşım. Selçuk Tıp Derg. 2013;29(1):42-8.