An Evaluation of Potent Radioprotective Effect of Dimethyl Sulfoxide for Acute Radiation-Induced Lung Injury: Tc99m-DTPA Transalveolar Clearance Scintigraphy Correlated by Histopathologic Findings

Yıl 2022, Cilt: 9 Sayı: 3, 266 - 272, 30.12.2022

Bengu Depboylu

https://doi.org/10.47572/muskutd.1076492

Öz

This study aims to investigate the radioprotective effects of dimethyl sulfoxide in the acute phase of radiation-induced lung injury by technetium-99m-diethylenetriaminepentaacetic acid transalveolar clearance scintigraphy in a rabbit model. Twenty white New Zealand rabbits were grouped as (1) control, (2) sham irradiation, (3) irradiation plus dimethyl sulfoxide, and (4) dimethyl sulfoxide alone. Right hemithorax regions of the rabbits in the sham irradiation and irradiation plus dimethyl sulfoxide groups were irradiated with a single dose of 20 Gy by a Cobalt60 treatment unit. Dimethyl sulfoxide (4.5 gr/kg) was administered intraperitoneally, 30 minutes before irradiation. The technetium-99m-diethylenetriaminepentaacetic acid transalveolar clearance scintigraphy was performed on the 14th day after irradiation. The rabbits were sacrificed on the 15th day, and both lungs were removed for histopathologic evaluation. Administration of dimethyl sulfoxide before irradiation caused a marked prolongation in the transalveolar clearance rate of diethylenetriaminepentaacetic acid through the alveolocapillary membrane (p=0.028), protected the anatomic ultrastructure of the lung parenchyma, markedly decreased the severity of exudate in the alveoli in histopathologic evaluation. Our study results showed that dimethyl sulfoxide has safely eliminated inflammatory response induced by irradiation while not causing any negative alterations in the structure of the normal lung tissue and preserving the integrity of the alveolocapillary membrane, especially in the acute phase. Dimethyl sulfoxide appears to be a safe and well-tolerated protective agent for the prevention of RILI. Technetium-99m-diethylenetriaminepentaacetic acid transalveolar clearance scintigraphy is accepted as a cheap, easily reproducible, sensitive assay to monitor radiation toxicity reactions in acute RILI.

Anahtar Kelimeler

dimethyl sulfoxide, radioprotective agent, radiation-induced acute lung injury, transalveolar clearance scintigraphy, radioprotector

Destekleyen Kurum

Trakya Üniversitesi

Proje Numarası

Protokol Numarası: TUTFEK-2007/83

Teşekkür

The author extends her gratitude to Prof. Dr. Zafer Koçak for his mentorship during the study.

Akut Radyasyona Bağlı Akciğer Hasarlanmalarında Dimetil Sülfoksitin Radyokoruyucu Etkisinin Tc99m-DTPA Transalveolar Klirens Sintigrafisi ve Histopatolojik Bulgularla Araştırılması

Öz

Bu çalışma, bir tavşan modelinde teknesyum-99m-dietilentriaminpentaasetik asit transalveolar klirens sintigrafisi ile radyasyona bağlı akciğer hasarının akut fazında dimetil sülfoksitin radyoprotektif etkilerini araştırmayı amaçlamaktadır. Yirmi beyaz Yeni Zelanda tavşanı (1) kontrol, (2) ışınlama, (3) ışınlama artı dimetil sülfoksit ve (4) tek başına dimetil sülfoksit şeklinde gruplandırıldı. Işınlama ve ışınlama artı dimetil sülfoksit gruplarındaki tavşanların sağ hemitoraks bölgeleri Cobalt60 tedavi ünitesi ile tek doz 20 Gy ışınlandı. Işınlamadan 30 dakika önce intraperitoneal yolla dimetil sülfoksit (4.5 gr/kg) uygulandı. Teknesyum-99m-dietilentriaminpentaasetik asit transalveolar klirens sintigrafisi ışınlamadan sonraki 14. günde yapıldı. Tavşanlar 15. günde sakrifiye edildi ve histopatolojik değerlendirme için her iki akciğer çıkarıldı. Işınlamadan önce dimetil sülfoksitin uygulanması, histopatolojik değerlendirmede dietilentriaminpentaasetik asidin alveolokapiller membrandan transalveolar klirens hızında belirgin bir uzamaya neden oldu (p=0.028), akciğer parankiminin anatomik yapısını korudu, alveollerdeki eksüda şiddetini belirgin şekilde azalttı (p=0.042). Çalışma sonuçlarımız, dimetil sülfoksitin, normal akciğer dokusunun yapısında olumsuz bir değişikliğe neden olmadan ve özellikle akut fazda alveolokapiller membranın bütünlüğünü koruyarak, ışınlamaya bağlı inflamatuar yanıtı güvenli bir şekilde ortadan kaldırdığını göstermiştir. Dimetil sülfoksit, RILI'nin önlenmesi için güvenli ve iyi tolere edilen bir koruyucu ajan gibi görünmektedir. Teknesyum-99m-dietilentriaminpentaasetik asit transalveolar klirens sintigrafisi, akut RILI'de radyasyon toksisitesini izlemek için ucuz, kolay tekrarlanabilir, hassas bir test olarak kabul edilmektedir.

Anahtar Kelimeler

dimetil sülfoksit, radyoprotektif ajan, radyasyona bağlı akut akciğer hasarı, transalveolar klirens sintigrafisi, radyoprotektör

Proje Numarası

Protokol Numarası: TUTFEK-2007/83

Kaynakça

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