Antioxidant Role of Beta-glucan on Cisplatin-induced Liver Injury in Rats

Öz

Objective: Cisplatin (cisdiamminedichloridoplatinum II [CDDP]) is an antineoplastic cancer drug frequently used in the treatment of a wide variety of cancers. In addition to its positive effects, it may have toxic effects in tissues such as hepatoxicity. In this study, we investigated the reparative effects of beta-glucan, a potent antioxidant, on cisplatin induced hepatoxicity. Method: In this study, 40 Wistar Albino rats were randomly divided into 2 groups according to the time of sacrifice, 3rd day and 5th day (n=20 in each group). Each group was divided into four groups as control, Beta-glucan (100 mg/kg/bw), cisplatin (10 g/kg/bw), cisplatin+betaglucan (n=5 in each group). The rats were sacrificed 3 days and 5 days after the last injections and liver tissue samples were taken for routine tissue follow-up for histopathologic examination under light microscope. Paraffin blocks were sectioned and stained with Hemaoxylin-Eosin. Results: Histopathological examination revealed different degrees of hydropic degeneration, sinusoidal expansion, disorganization of hepatic cords, inflammatory cell foci and changes in the density of Kupffer cells in cisplatin 3rd day and cisplatin 5th day groups compared to the control group. When cisplatin was administered together with beta-glucan, cell damage was found to be minimal in cisplatin+betaglucan 3rd and cisplatin+beta-glucan 5th day groups. Conclusion: Light microscopic findings suggest that beta-glucan may have protective effects on cisplatin-induced liver injury.

Anahtar Kelimeler

• Antioxidant
• Beta-glucan
• Cisplatin
• Liver Injury

Kaynakça

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