Chemical composition and antibacterial activity of Clinopodium nepeta subsp. glandulosum (Req.) Govaerts essential oil

Yıl 2021, Cilt: 8 Sayı: 3, 75 - 80, 30.09.2021

Gözde Öztürk , Gülderen Yılmaz , Gülnur Ekşi , Betül Demirci

https://doi.org/10.37929/nveo.949959

Cited By: 5

Öz

Clinopodium nepeta subsp. glandulosum (Req.) Govaerts is a natural perennial grass belonging to the Lamiaceae family, growing spontaneously on the Mediterranean coast. It is locally known as “kedi fesleğeni” in Turkey. This aromatic plant is used as a mint-like spice in food preparations and in the composition of some recipes during religious feasts. In folk medicine, it is used as stimulant, antiseptic and antispasmodic. Aerial parts of C. nepeta subsp. glandulosum were collected from Bilecik in July 2017, and was air dried. The essential oil was isolated by hydrodistillation using a Clevenger-type apparatus and the composition of the essential oil was simultaneously analysed by GC-FID and GC-MS. Piperitenone oxide (47.8%), limonene (18.6%) and piperitone oxide II (13.6%) were found as major components. Antibacterial activity of the essential oil was tested against Escherichia coli NRRLB-3008, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Bacillus cereus NRRL-B3711 and Streptococcus sanguinis ATCC 10556 were used by broth microdilution method. Ciprofloxacin was used as control and Minimal Inhibitory Concentrations (MIC) were determined. The highest activity was found against S. typhimurium (1250 µg/mL). The essential oil is more effective against B. cereus (2500 µg/mL) and S. sanguinis (2500 µg/mL). The lowest activities were determined against E. coli (5000 µg/mL) and P. aeruginosa (10000 µg/mL).

Anahtar Kelimeler

Lamiaceae, Clinopodium nepeta subsp. glandulosum, essential oil, chemical composition, antibacterial activity

Kaynakça

• Arantes, S.M., Piçarra, A., Guerreiro, M., Salvador, C., Candeias, F., Caldeira, A.T., & Martins, M.R. (2019). Toxicological and pharmacological properties of essential oils of Calamintha nepeta, Origanum virens and Thymus mastichina of Alentejo (Portugal), Food and Chemical Toxicology, 133, 110747.
• Baytop, T. (1999). Therapy with Medicinal Plants in Turkey. Istanbul: Nobel Tıp Basımevi.
• Brankovic, S.V., Kitic, D.V., Radenkovic, M.M., Veljkovic, S.M., & Golubovic, T.D. (2009). Calcium blocking activity as a mechanism of the spasmolytic effect of the essential oil of Calamintha glandulosa Silic on the isolated rat ileum. Gen Physiol Biophys., 28,174-178.
• Conforti, F., Marrelli, M., Statti, G., Menichini, F., Uzunov, D., Solimene, U., & Menichini, F. (2012). Comparative chemical composition and antioxidant activity of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) Nyman and Calamintha grandiflora (L.) Moench (Labiatae), Natural Product Research, 26(1), 91-97.
• Davis, P.H. (1982). Flora of Turkey and East Aegean Islands. Edinburg: University Press
• Debbabi, H., Mokni, R. E., Chaieb, I., Nardoni, S., Maggi, F., Caprioli, G., & Hammami, S. (2020). Chemical Composition, Antifungal and Insecticidal Activities of the Essential Oils from Tunisian Clinopodium nepeta subsp. nepeta and Clinopodium nepeta subsp. glandulosum. Molecules, 25(9), 2137.
• Demirci, B., Temel, H. E., Portakal, T., Kirmizibekmez, H., Demirci, F., & Başer, K. H. C., (2011). Inhibitory effect of Calamintha nepeta subsp glandulosa essential oil on lipoxygenase. Turkish Journal of Biochemistry, 36(4), 290-295.
• Demirci, F., Güven, K., Demirci, B., Dadandı, & M.Y. & Baser, K.H.C. (2008). Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control, 19(12), 1159-1164.
• Dzhambazov, B., Daskalova, S., Monteva, A., & Popov, N. (2002). In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts. Biological and Pharmaceutical Bulletin, 25(4), 499-504.
• Govaerts R. (1999). World checklist seed plants 3. Continental Publishing, Deurne.
• Jang, J., Hur, H.G., Sadowsky, M.J., Byappanahalli, M.N., Yan, T., & Ishii, S. (2017). Environmental Escherichia coli: ecology and public healthimplications—a review, Journal of Apply Microbiology, 123, 570-581.
• Kitic, D., Jovanovic, T., Ristic, M., Palic, R., & Stojanovic, G. (2002). Chemical Composition and Antimicrobial Activity of the Essential Oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball from Montenegro, J. Essent. Oil Res., 14, 150-152. Kotiranta, A., Lounatmaa, K., & Haapasalo, M. (2000). Epidemiology and pathogenesis of Bacillus cereus infections. Microb Infect, 2(2), 189-198.
• Kreth, J., Merritt, J., Shi, W., & Qi, F. (2005). Competition and Coexistence between Streptococcus mutans and Streptococcus sanguinis in the Dental Biofilm. J. of Bacteriology, 187(21), 7193–7203.
• Marongiua, B., Piras, A., Porcedda, S., Falconieri, D., Maxia, A., Gonçalves, M.J., Cavaleiro, C., & Salgueiro, L. (2010). Chemical composition and biological assays of essential oils of Calamintha nepeta (L.) Savi subsp. nepeta (Lamiaceae), Natural Product Research, 24(18), 1734-1742.
• Miladinović, D.L., Ilić, B.S., Mihajilov-Krstev, T.M., Nikolic, N.D., Miladinovic, L.C., & Cvetkovic, O.G. (2021) Investigation of the chemical composition–antibacterial activity relationship of essential oils by chemometric methods. Anal Bioanal Chem 403, 1007–1018.
• Patricia, I.F., Ronald, S., Constantine, H., & Fred, H. (1986). Mutants of Salmonella typhimurium that cannot survive within the macrophage are a virulent. Proc Natl Acad Sci, 83, 5189-5193.
• Pfaller, M.A., Chaturvedi, V., Diekema, D.J., Ghannoum, M.A., Holliday, N.M., Killian, S.B., Knapp C.C., Messer, S.A., Miskov. A., & Ramani, R. (2008). Clinical evaluation of the Sensititre Yeast One colorimetric antifungal panel for antifungal susceptibility testing of the echinocandins anidulafungin, caspofungin, and micafungin. Journal of Clinical Microbiology, 46(7), 2155-2159. Sarac, N., & Ugur, A. (2009). The in vitro antimicrobial activities of the essential oils of some Lamiaceae species from Turkey. J Med Food. 12(4), 902-907.
• Şarer, E., & Pançalı, S.S. (1998). Composition of the Essential Oil from Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball, Flavour and Fragrance Journal, 13, 31-32.
• Tepe, B., Sihoglu-Tepe, A., Daferera, D., Polissiou, M., & Sokmen, A. (2007). Chemical composition and antioxidant activity of the essential oil of Clinopodium vulgare L. Food Chemistry, 103(3), 766-770.
• Wolska, K., & Szweda, P. (2009). Genetic Features of Clinical Pseudomonas aeruginosa Strains. Polish J Microbiol 58(3), 255-260.