The Free Radical Scavenging Activity of Crithmum maritimum L. from the Blacksea Cost

Öz

Objective: There is a great deal of research on nutraceuticals and natural compounds obtained by plant extraction to promote health and reduce disease risk. Crithmum maritimum L. (C. maritimum) from Apiaceae family, also known as the sea fennel, is edible halophyte. This halophyte, made of pickles and consumed among the population, has a high secondary metabolite content. The purpose of this study is to evaluate the free radical scavenging activity of the leaves of C. maritimum.
Methods: Aerial parts of C. maritimum were collected from the shores of Giresun in May 2018. The leaves of the plant were washed respectively with fresh water and then with distilled water. After they were dried at room temperature in the dark, dried aerial parts were grounded. Ethanol and methanol extracts were prepared of the C. maritimum by using soxhlet and evaporator. Then extracts were lyophilized. To determine of free radical scavenging activity, DPPH (1,1-diphenyl-2-picrylhydrazyl) method was applied as spectrophotometrically.
Results: The results were compared with the other standard antioxidants (butylated hydroxytoluene and α-tocopherol). Ethanol and methanol extracts of C. maritimum has high radical scavenging activity.
Conclusion: C. maritimum leaves, which are known as sea fennel consumed as food, can also be considered as functional food due to its high radical scavenging effect.

Anahtar Kelimeler

• Crithmum maritimum L.
• free radical scavenger
• extract

Kaynakça

1. 1. Atia A, Barhoumi Z, Mokded R, Abdelly C, Smaoui A. Environmental eco-physiology and economical potential of the halophyte Crithmum maritimum L. (Apiaceae). J Med Plants Res, 2011;5:3564–3571.
2. 2. Renna M. Reviewing the Prospects of Sea Fennel (Crithmum maritimum L.) as Emerging Vegetable Crop. Plants (Basel, Switzerland), 2018:7(4), 92. https://doi.org/10.3390/plants7040092
3. 3. Siracusa L, Kulisic-Bilusic T, Politeo O, Krause I, Dejanovic B, & Ruberto G. Phenolic composition and antioxidant activity of aqueous infusions from Capparis spinosa L. and Crithmum maritimum L. before and after submission to a two-step in vitro digestion model. Journal of agricultural and food chemistry, 2011;59(23):12453–12459. https://doi.org/10.1021/jf203096q 4. Cunsolo F, Ruberto G, Amico V, Piattelli M. Bioactive metabolites from Sicilian marine fennel, Crithmum maritimum. J Nat Prod, 1993;56:1598–1600. 5. Kulisic-Bilusic T, Blažević I, Dejanović B, Miloš M. And Pifat G. Evaluation Of The Antioxidant Activity Of Essential Oils From Caper (Capparis Spinosa) And Sea Fennel (Crithmum Maritimum) By Different Methods. Journal of Food Biochemistry, 2010;34: 286-302. https://doi.org/10.1111/j.1745-4514.2009.00330.x 6. Cebi A. Determination of Total Antioxidant Capacities by Ferrous Thiocyanate Method in Leaf And Seed of Cherry Laurel (Prunus laurocerasus L). The Black Sea Journal of Sciences, 2018;8(1):109-114.
4. 7. Blois MS. Antioksidant determinations by the use of a stable free radical. Nature, 1958;26: 1199–1200.
5. 8. Cebi A, Akgun E, Celikler S, Firat M, Ozel MZ, Ulukaya E, & Ari F. Cytotoxic and genotoxic effects of an endemic plant of Turkey Salvia kronenburgii on breast cancer cell lines. Journal of cancer research and therapeutics, 2019;15(5): 1080–1086. https://doi.org/10.4103/jcrt.JCRT_1322_16
6. 9. Renna M, Gonnella M. The use of the sea fennel as a new spice-colorant in culinary preparations. Int. J.Gastron. Food Sci., 2012;1:111–115.
7. 10. Jallali I, Zaouali Y, Missaoui I, Smeoui A, Abdelly, C, & Ksouri R. Variability of antioxidant and antibacterial effects of essential oils and acetonic extracts of two edible halophytes: Crithmum maritimum L. and Inula crithmoїdes L. Food Chemistry, 2014;145:1031–1038. https://doi.org/10.1016/j.foodchem.2013.09.034
8. 11. Sousa G, Alves MI, Neves M, Tecelão C, & Ferreira-Dias S. (2022). Enrichment of Sunflower Oil with Ultrasound-Assisted Extracted Bioactive Compounds from Crithmum maritimum L. Foods (Basel, Switzerland), 2022;11(3):439.https://doi.org/10.3390/foods11030439

9. 12. Gnocchi D, Cesari G, Calabrese GJ. Capone R, Sabba C, Mazzocca A. Inhibition of Hepatocellular Carcinoma Growth by Ethyl Acetate Extracts of Apulian Brassica oleracea L. and Crithmum maritimum L. Plant Foods Hum Nutr 2020;75:33–40.
10. 13. Souid A, Della Croce CM, Frassinetti S, Gabriele M, Pozzo L, Ciardi M, et. al. Nutraceutical Potential of Leaf Hydro-Ethanolic Extract of the Edible Halophyte Crithmum maritimum L. Molecules (Basel, Switzerland), 2021;26(17): 5380. https://doi.org/10.3390/molecules26175380.