Peroksidaz Enzimi ile Asidik ve Reaktif Boyarmaddelerin Rensizleştirilmesinde 1-hidroksibenzotriazolün Etkisinin Araştırılması

Yıl 2023, Cilt: 8 Sayı: 2, 74 - 83, 21.12.2023

Mithat Çelebi , Melda Altıkatoğlu

https://doi.org/10.56171/ojn.1364230

Öz

Boyar içeren tekstil boyama atıksuları, renkleri ve tehlikeli veya kanserojen ara maddelerin üretimi nedeniyle nehirleri kirletmekte olup, bu atık suların akarsulara bırakılmadan önce uzaklaştırılması gerekmektedir. Ligninolitik enzimlerin, endüstriyel atık sulardan renklerin giderilmesi de dahil olmak üzere birçok kullanım alanı vardır. Bu çalışmada, Lignin Peroksidaz enzimi ile Naftol Blue Black ((NBB) ve Remazol Brillant Blue R (RBBR) boyarmaddeleri pH:5.0 ve 30 °C’de giderildi. Ayrıca, bir redoks mediatörü olan 1-hidroksibenzotriazol (HBT)’ün farklı konsantrasyonlarda boyaların giderilmesine etkisi incelendi. LiP enzimine bir redoks mediatörü eklendiğinde, giderilen RBBR boyasının yüzdesi 24 saat sonra %10'dan %26'ya yükseldi. HOBt'nin LiP enzimine eklenmesinden sonraki ilk günde, elimine edilen NBB boyasının yüzdesi %5'ten %89'a yükseldi. HOBt’nin etkisi NBB boyasında RBBR boyasına göre daha belirgin gözlendi. Redoks mediatörü katkılı Lignin Peroksidaz enzimi, NBB boyarmaddesini RBBR boyarmaddesine göre daha yüksek boya giderme değerleri ile giderdi.

Anahtar Kelimeler

Lignin Peoksidaz, 1-hidroksibenzotriazol (HOBt), renk giderme, o-dinaisidine, boyar madde

The Effect of 1-hydroxybenzotriazole on the Decolorization of Anthraquinone and Diazo Dyes Using Peroxidase Enzyme

Öz

Textile dyeing wastewater involving resistant dyestuffs contaminate rivers because of their color and the production of hazardous or carcinogenic intermediates, which must be removed before being released into streams. The ligninolytic enzymes have many uses, including the elimination of dyestuffs from industrial wastewater. In this study, the Lignin Peroxidase enzyme was used to eliminate synthetic solutions of Naphthol Blue Black (NBB) and Remazol Brilliant Blue R (RBBR) dyestuffs at pH 5.0 and 30 °C. In addition, the effect of 1-hydroxybenzotriazole (HOBt), a redox mediator, on dye removal at different concentrations was investigated. Once a redox mediator was added to the LiP enzyme, the percentage of RBBR dye eliminated increased from 10% to 26% after 24 hours. On the first day after adding HOBt to the LiP enzyme, the percentage of NBB dye eliminated increased from 5% to 89%. The effect of HOBt was significantly greater in the NBB than in the RBBR. The redox mediator-added Lignin Peroxidase enzyme decolorized the NBB dye more efficiently than the RBBR dye.

Anahtar Kelimeler

Lignin Peroxidase, 1-hydroxybenzotriazole (HOBt), decolorization, o-dianisidine, dyestuff

Kaynakça

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