Development and In Vitro Characterization of Nanoemulsion Containing Hippophaes Rhamnoides Aqueous Extract

Abstract

Aim: Hippophaes rhamnoides is a member of the Elaeagnaceae family and grows in many countries of world such as Turkey, France, Germany, Norway and Russia. Hippophaes rhamnoides extracts has antioxidant, anticancer, antidiabetic, antiviral and cardiovascular protective effect. Nanoemulsions (NEs) are suitable systems for formulations of herbal extracts. The aim of this study is to develop and in vitro characterize NE formulation containing Hippophaes rhamnoides aqueous extract (AE-Hr) . Material and Method: AE-Hr-containing (AE-Hr-NE) or blank (B-NE) NE formulations were prepared using ethyl oleate, Lipoid S100, Kollphor RH40, Pluronic F68, DMSO, and ultrapure water. The droplet size, PDI and zeta potential values of NEs were determined and pH measurement, FT-IR and rheological analyzes were also performed. Results: The droplet size and PDI values of NEs were found < 200 nm and < 0.3, which indicates monodispersity. Also the zeta potential values of B-NE and AE-Hr-NE were determined as (-)28.51±2.61 and (-)30.11±2.02 mV, respectively. FT-IR results showed that the extract is completely dissolved in the oil phase of formulations. Conclusion: The NE formulation containing AE-Hr was successfully prepared and characterized. The formulation may be beneficial for usage of Hippophaes rhamnoides aqueous extract.

Keywords

• Hippophaes rhamnoides
• in vitro characterization
• nanoemulsion

Kaynakça

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