DEVELOPMENT AND IN VITRO CHARACTERIZATION OF NANOEMULSION AND NANOEMULSION BASED GEL CONTAINING ARTEMISIA DRACUNCULUS ETHANOL EXTRACT

Yıl 2022, Cilt: 2 Sayı: 2, 36 - 43, 06.04.2022

Ayse Sena Atmaca Yasar Furkan Kılınboz Afife Büşra Uğur Kaplan Meltem Çetin

Öz

Aim: Tarragon, or Artemisia dracunculus, is a member of the Asteraceae family that grows wild in Eastern Anatolia of Turkey. According to previously published studies, Artemisia dracunculus extracts possess antibacterial, antifungal, antioxidant and anti-inflammatory effects. Thus, these extracts can be used to heal wounds. Nanoemulsion (NE) is a suitable dosage form for the application of active substances/compounds via the skin. The aim of this study is to develop and in vitro characterize NE and NE-based gel formulations containing Artemisia dracunculus ethanol extract.
Methods: Extract-containing (E-NE) or blank (B-NE) NE formulations were prepared using ethyl oleate, Lipoid S100, Tween 80, Pluronic F127, DMSO, and ultrapure water. NaCMC was added to NE formulations to obtain NE-based gels (NEGs). The droplet size, PDI and zeta potential values of NEs were determined; pH measurement, FT-IR and rheological analyzes were also performed for NEs and NEGs.
Results: The droplet size and zeta potential values of B-NE and E-NE were found as 139.13±5.15 nm and 135.59±4.81 nm, (-)27.53±2.05 mV and (-)26.28±3.21 mV, respectively. Also, PDI values of NE formulations were <0.3, indicating monodispersity. The pH values of NEs and NEGs were found in the range of 4.26±0.10 - 6.16±0.03, using suitably for topical application. In addition, NEGs formulations showed a pseudoplastic behavior which is important for the topical application. FT-IR results showed that the extract is completely dissolved in the oil phase of formulations.
Conclusion: The NE and NEG may be useful for the topical application of Artemisia dracunculus ethanol extract.

Anahtar Kelimeler

Artemisia dracunculus, extract, nanoemulsion, nanoemulsion-based gel, topical application.

Teşekkür

Yasar Furkan KILINBOZ would like to thank to the scholarship along his postgraduate program provided by the Turkish Scientific and Technical Research Council (TUBITAK).

Kaynakça

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