Development and Validation of HPLC Method for the Determination of Flurbiprofen in Pharmaceutical Preparations

Yıl 2022, Cilt: 2 Sayı: 3, 72 - 81, 06.07.2022

Bilal Yılmaz , Burak Bayrak , Yücel Kadıoğlu

Öz

Bu çalışmada, saf ve farmasötik preparatlarda flurbiprofen tayini için yeni ve hızlı yüksek performanslı sıvı kromatografisi (HPLC) yöntemi geliştirilmiştir. Yöntem, fosforik asit ile pH 3.5'e ayarlanmış bir asetonitril-0.05 M potasyum dihidrojen fosfat çözeltisinin (60:40, v/v) mobil fazı kullanılarak Ace C18 kolonu üzerinde geliştirildi. Eluent 254 nm'de UV dedektör ile izlendi. Analiz, 1.0 mL dak-1 akış hızıyla 6 dakikadan daha kısa sürede gerçekleştirildi. Kalibrasyon eğrisi 0.10-5.0 μg mL-1 konsantrasyon aralığında doğrusaldı. Flurbiprofen için gün içi ve günler arası kesinlik değerleri 4.56'dan azdı ve doğruluk (bağıl hata) %4.00'den daha iyiydi. Farmasötik preparatlar için flurbiprofenin ortalama geri kazanımı %99.8 idi. Gözlenebilme (LOD) ve miktar tayin (LOQ) limitleri sırasıyla 0,03 ve 0,10 µg mL-1 idi. Ayrıca yöntem ilacın miktar tayinini yapmak ve formülasyon içeriği tekdüzeliğini kontrol etmek için iki ticari flurbiprofen dozaj formunun kalite kontrolü için uygulandı.

Anahtar Kelimeler

Flurbiprofen, HPLC, Pharmaceutical preparation, Validation

Kaynakça

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