Araştırma Makalesi
BibTex RIS Kaynak Göster
Yıl 2022, Cilt: 2 Sayı: 1, 11 - 15, 25.01.2022

Öz

Kaynakça

  • 1. Burtis CA, Bruns DE, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7. baskı. 2015, St. Louis Missouri: Elsevier Saunders.
  • 2. Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, et al, Anti-inflammatory activity of N-butanol extract from Ipomoea stolonifera in vivo and in vitro. PLoS One. 2014; 9; e95931.
  • 3. World Healty Organisation. Cancer. 2017 [21.10.2017]; Available from: http://www.who.int/mediacentre/factsheets/fs297/en/.
  • 4. Bignold L, Principles of Tumors. 1. baskı. 2015, New York: Academic Press.
  • 5. T.C.Sağlık Bakanlığı. Türkiye Kanser İstatistikleri. 2016 [21.10.2017]; Available from: http://kanser.gov.tr/daire-faaliyetleri/kanser-istatistikleri.
  • 6. Suh Y, Afaq F, Khan N, Johnson JJ, Khusro FH, Mukhtar H, Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells. Carcinogenesis. 2010; 31; 1424-33.
  • 7. Maher P, A comparison of the neurotrophic activities of the flavonoid fisetin and some of its derivatives. Free Radic Res. 2006; 40; 1105-11.
  • 8. Higa S, Hirano T, Kotani M, Matsumoto M, Fujita A, Suemura M, et al, Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils. J Allergy Clin Immunol. 2003; 111; 1299-306.
  • 9. Kashyap D, Sharma A, Sak K, Tuli HS, Buttar HS, Bishayee A, Fisetin: A bioactive phytochemical with potential for cancer prevention and pharmacotherapy. Life Sci. 2018; 194; 75-87.
  • 10. Prasath GS, Subramanian SP, Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats. Food Chem Toxicol. 2013; 59; 249-55.
  • 11. Pal HC, Athar M, Elmets CA, Afaq F, Fisetin inhibits UVB-induced cutaneous inflammation and activation of PI3K/AKT/NFkappaB signaling pathways in SKH-1 hairless mice. Photochem Photobiol. 2015; 91; 225-34.
  • 12. Sakai E, Shimada-Sugawara M, Yamaguchi Y, Sakamoto H, Fumimoto R, Fukuma Y, et al, Fisetin inhibits osteoclastogenesis through prevention of RANKLinduced ROS production by Nrf2-mediated up-regulation of phase II antioxidant enzymes. J Pharmacol Sci. 2013; 121; 288-98.
  • 13. Suh Y, Afaq F, Johnson JJ, Mukhtar H, A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-kappaBsignaling pathways. Carcinogenesis. 2009; 30; 300-7.
  • 14. Prakash D, Gopinath K, Sudhandiran G, Fisetin enhances behavioral performances and attenuates reactive gliosis and inflammation during aluminum chloride-induced neurotoxicity. Neuromolecular Med. 2013; 15; 192-208.
  • 15. Hemanth Kumar B, Arun Reddy R, Mahesh Kumar J, Dinesh Kumar B, Diwan PV, Effects of fisetin on hyperhomocysteinemia-induced experimental endothelial dysfunction and vascular dementia. Can J Physiol Pharmacol. 2017; 95; 32-42.
  • 16. Lee SE, Jeong SI, Yang H, Park CS, Jin YH, Park YS, Park. Fisetin induces Nrf2- mediated HO-1 expression through PKC-delta and p38 in human umbilical vein endothelial cells. J Cell Biochem. 2011; 112; 2352-60.

The Effect of Fisetin on KATO-III cell proliferation and invasion

Yıl 2022, Cilt: 2 Sayı: 1, 11 - 15, 25.01.2022

Öz

Fisetin is a natural chemical that widely found in different plants. The aim of this study is to investigate the role of fisetin on proliferation and invasion on KATO-III human gastric carcinoma cell. KATO-III cells were seeded into the plates and 24h after dose-dependent effects of fisetin (50 and 100µM) were tested on cultured KATO-III cells. Real-time cell proliferation impedance analysis was performed up to 72h and the Transwell insert assay was performed. Relative mRNA expressions of MMP2 and MMP9, which are markers of invasion, were measured. Data are presented as fold-change in expression of any group compared to that of control group, using the 2-ΔΔCt method. Statistical comparisons were made using oneway ANOVA followed by Tukey's test. Fisetin exert anti-proliferative and anti-invasion effect on KATO-III cells. According to the cell proliferation impedance results, it was shown that proliferations of all fisetin treated groups were suppressed in dose dependent manner. According to transwell invasion results, 100µM fisetin group showed important inhibitory effects on invasion of KATO-III cells compared to the Control group. Regarding to the mRNA expression results of MMP2 and MMP9, it was shown that 50 and 100µM fisetin treatments significantly decreased these expressions compared to Control group. We conclude that fisetin negatively regulates the invasion and proliferation potentials of KATO-III cells via the MMP9 and MMP2 suppression. Thus, our findings implicate fisetin a potential therapeutic target in KATO-III cells.

Kaynakça

  • 1. Burtis CA, Bruns DE, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7. baskı. 2015, St. Louis Missouri: Elsevier Saunders.
  • 2. Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, et al, Anti-inflammatory activity of N-butanol extract from Ipomoea stolonifera in vivo and in vitro. PLoS One. 2014; 9; e95931.
  • 3. World Healty Organisation. Cancer. 2017 [21.10.2017]; Available from: http://www.who.int/mediacentre/factsheets/fs297/en/.
  • 4. Bignold L, Principles of Tumors. 1. baskı. 2015, New York: Academic Press.
  • 5. T.C.Sağlık Bakanlığı. Türkiye Kanser İstatistikleri. 2016 [21.10.2017]; Available from: http://kanser.gov.tr/daire-faaliyetleri/kanser-istatistikleri.
  • 6. Suh Y, Afaq F, Khan N, Johnson JJ, Khusro FH, Mukhtar H, Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells. Carcinogenesis. 2010; 31; 1424-33.
  • 7. Maher P, A comparison of the neurotrophic activities of the flavonoid fisetin and some of its derivatives. Free Radic Res. 2006; 40; 1105-11.
  • 8. Higa S, Hirano T, Kotani M, Matsumoto M, Fujita A, Suemura M, et al, Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils. J Allergy Clin Immunol. 2003; 111; 1299-306.
  • 9. Kashyap D, Sharma A, Sak K, Tuli HS, Buttar HS, Bishayee A, Fisetin: A bioactive phytochemical with potential for cancer prevention and pharmacotherapy. Life Sci. 2018; 194; 75-87.
  • 10. Prasath GS, Subramanian SP, Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats. Food Chem Toxicol. 2013; 59; 249-55.
  • 11. Pal HC, Athar M, Elmets CA, Afaq F, Fisetin inhibits UVB-induced cutaneous inflammation and activation of PI3K/AKT/NFkappaB signaling pathways in SKH-1 hairless mice. Photochem Photobiol. 2015; 91; 225-34.
  • 12. Sakai E, Shimada-Sugawara M, Yamaguchi Y, Sakamoto H, Fumimoto R, Fukuma Y, et al, Fisetin inhibits osteoclastogenesis through prevention of RANKLinduced ROS production by Nrf2-mediated up-regulation of phase II antioxidant enzymes. J Pharmacol Sci. 2013; 121; 288-98.
  • 13. Suh Y, Afaq F, Johnson JJ, Mukhtar H, A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-kappaBsignaling pathways. Carcinogenesis. 2009; 30; 300-7.
  • 14. Prakash D, Gopinath K, Sudhandiran G, Fisetin enhances behavioral performances and attenuates reactive gliosis and inflammation during aluminum chloride-induced neurotoxicity. Neuromolecular Med. 2013; 15; 192-208.
  • 15. Hemanth Kumar B, Arun Reddy R, Mahesh Kumar J, Dinesh Kumar B, Diwan PV, Effects of fisetin on hyperhomocysteinemia-induced experimental endothelial dysfunction and vascular dementia. Can J Physiol Pharmacol. 2017; 95; 32-42.
  • 16. Lee SE, Jeong SI, Yang H, Park CS, Jin YH, Park YS, Park. Fisetin induces Nrf2- mediated HO-1 expression through PKC-delta and p38 in human umbilical vein endothelial cells. J Cell Biochem. 2011; 112; 2352-60.
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri
Bölüm Research Articles
Yazarlar

Rüstem Anıl Uğan

Harun Ün 0000-0003-1772-282X

Yayımlanma Tarihi 25 Ocak 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 2 Sayı: 1

Kaynak Göster

EndNote Uğan RA, Ün H (01 Ocak 2022) The Effect of Fisetin on KATO-III cell proliferation and invasion. International Journal of PharmATA 2 1 11–15.