Tarçının Deneysel Olarak İndüklenen Diyabetli Sıçanların Akciğer Dokusunda VEGF ve NF-κB İmmünoreaksiyonu Üzerindeki Etkileri

Yıl 2022, , 72 - 77, 01.07.2022

Tuğrul Ertuğrul , Gökçen Sevilgen

https://doi.org/10.38175/phnx.1103944

Öz

Amaç: Diabetes mellitus, insülin eksikliği veya direncinin neden olduğu hiperglisemi olarak tanımlanan metabolik bir hastalıktır. Çalışmalarda artan kanıtlar, akciğerin diyabetik komplikasyonların hedefi olduğunu göstermiştir. Geleneksel tıp teorilerine göre tarçın, şeker hastaları için destekleyici bir tedavi yöntemi olarak kabul edilmektedir. Bu çalışmanın amacı, tarçının streptozotosin ile indüklenen deneysel diyabetik sıçanların akciğer dokusunda VEGF ve NF-κB'nin immünohistokimyasal ekspresyonu üzerindeki etkisini araştırmaktır.
Gereç ve Yöntem: Otuz iki erkek sıçan rastgele dört gruba ayrıldı: Diyabet, Diyabet + tarçın, Tarçın ve Kontrol. VEGF ve NF-κB'nin akciğer dokusunda immünohistokimyasal ekspresyonu, streptavidin-biotin kompleksi kullanılarak belirlendi.
Bulgular: Tarçın uygulamasının tek başına akciğer dokusunda VEGF ekspresyonunu değiştirmezken, diyabet grubunda tarçın uygulaması ile azalmış VEGF ekspresyonunun arttığı belirlendi. Gruplardaki immün boyama incelendiğinde, kontrol ve tarçın grupları arasında NF-κB immünreaksiyonu açısından bir fark gözlenmedi. Dikkat çekici bir bulgu olarak, diyabetik grubun akciğer dokusunda güçlü pozitif NF-κB reaksiyonları vardı. Ayrıca diyabet+cinnamom grubunda zayıf pozitif NF-κB reaksiyonu tespit edildi.
Sonuç: Sonuç olarak bizim çalışmamızda tarçın diyabetin neden olduğu NF-κB ekspresyonundaki artışın azalmasına, azalmış VEGF ekspresyonunun ise artmasına neden olmuştur. Sonuç olarak, bu çalışmanın diyabete bağlı olarak akciğer dokusunda meydana gelebilecek olası sitokin mekanizması değişikliklerinin anlaşılmasında ve tedavi yöntemlerinin geliştirilmesinde faydalı olacağı kanaatindeyiz.

Anahtar Kelimeler

Akciğer, Diyabet, NF-KB, VEGF, Tarçın

Effects of Cinnamon on VEGF and NF-κB Immunoreaction in The Lung Tissue of Rats with Experimentally Induced Diabetes

Öz

Objective: Diabetes mellitus is a metabolic disorder described as hyperglycemia induced by insulin deficiency or resistance. Increasing evidence in studies has shown that the lung is the target of diabetic complications. According to traditional medicine theories, cinnamon is considered a supportive treatment method for diabetics. The aim of this study is to investigate the effect of cinnamon on the immunohistochemical expression of VEGF and NF-κB in lung tissue of streptozotocin-induced experimental diabetic rats.
Material and Method: Thirty-two male rats were randomly divided into four groups: Diabetes, Diabetes + cinnamon, Cinnamon, and Control. The immunohistochemical expression of VEGF and NF-κB in the lung tissue was determined by using the streptavidin-biotin complex method.
Results: It was determined that while cinnamon application alone did not change VEGF expression in lung tissue, the decreased VEGF expression in the diabetes group increased with the cinnamon application. When the immunostaining in the groups was examined no difference was observed in NF-κB immunoreaction between the control and cinnamon groups. As a remarkable finding, in the diabetic group's lung tissue, there were strong positive NF-κB reactions. In addition, a weak positive NF-κB reaction was detected in the diabetes+cinnamom group.
Conclusion: As a result, in our study cinnamon caused decreased the increase in NF-κB expression caused by diabetes and increased the decreased VEGF expression. In conclusion, we believe that this study will be useful in understanding possible cytokine mechanism changes that may occur in the lung tissue due to diabetes and in the development of treatment methods.

Anahtar Kelimeler

Cinnamon, Diabetes, Lung, NF-KB

Kaynakça

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