The Effect of Rosmarinic Acid Against Ovarian and Lung Injuries Induced by Ovarian Torsion Detorsion in Rats

Abstract

Background: Here, we purposed to find out the effects of two different Rosmarinic acid (RA) doses against lung injury caused by ovarian ischemia-reperfusion. Materials and Methods: We planned the groups as sham, ovarian torsion detorsion (O/TD; 3hours torsion/3hours detorsion), RA 40 mg/kg (40 mg/kg RA+O/TD), and RA 80 mg/kg (80 mg/kg RA+O/TD) groups. Following the experimental procedure, we sacrificed the rats and then, collected the lung and ovarian tissues for biochemical evaluations. Result: Total oxidant status (TOS), myeloperoxidase (MPO) activity, malondialdehyde (MDA) levels, and oxidative stress index (OSI) were elevated in the O/TD group compared to the sham group. These parameters declined due to low and high doses of RA administration. Total antioxidant status (TAS) level and superoxide dismutase (SOD) activity diminished in the O/TD group while increasing in RA treatment groups. However, the high dose of RA treatment group enhanced the antioxidant activity further and reduced the oxidant parameters compared to the low dose RA treatment group. Conclusion: In this study, RA treatment reduced O/TD-induced ovarian and lung injuries in the experimental animals.

Keywords

• Rosmarinic acid
• torsion detorsion
• ovary
• rat

Over Torsiyon Detorsiyon Kaynaklı Over ve Akciğer Hasarına Karşı Rosmarinik Asidin Etkisi

Abstract

Amaç: Bu çalışmada over torsiyon detorsiyonunun neden olduğu akciğer hasarına karşı iki farklı Rosmarinic asit (RA) dozunun etkilerini bulmayı amaçladık. Gereç ve Yöntem: Grupları sham, over torsiyon detorsiyon (O/TD; 3 saat torsiyon/3 saat detorsiyon), RA 40 mg/kg (40 mg/kg RA+O/TD) ve RA 80 mg/kg (80 mg/kg RA+O/TD) olarak planladık. Deneyin ardından sıçanları sakrifiye edip biyokimyasal değerlendirmeler için akciğer ve over dokularını aldık. Bulgular: Total oksidan statüsü (TOS), myeloperoksidaz (MPO) aktivitesi, malondialdehit (MDA) seviyeleri ve oksidatif stres indeksi (OSI), O/TD grubunda sham gruba kıyasla yükselmişti. Bu parametreler, düşük ve yüksek doz RA uygulaması sonucunda azalma gösterdi. Total antioksidan statüsü (TAS) düzeyi ve süperoksit dismutaz (SOD) aktivitesi, RA tedavi gruplarında artarken O/TD grubunda azaldı. Ancak, yüksek doz RA tedavi grubu, düşük doz RA tedavi grubuna kıyasla antioksidan aktiviteyi daha da arttırıp oksidan parametreleri azaltmıştır. Sonuç: Mevcut çalışmada RA tedavisi sonucu deney hayvanlarında O/TD'nin neden olduğu over ve akciğer hasarı azalmıştır.

Keywords

• Rosmarinik asit
• torsiyon detorsiyon
• over
• rat

References

1. 1. Oelsner G, Shashar D. Adnexal torsion. Clin Obstet Gynecol. 2006;49(3):459-63.
2. 2. Geimanaite L, Trainavicius K. Ovarian torsion in children: management and outcomes. J Pediatr Surg. 2013;48(9):1946-53.
3. 3. Bacanakgil BH, Kaban I, Devecİ M, Hasanova M. Ovarian Torsion: 10 Years’ Experience of a Tertiary Medical Center. Istanb Med J. 2018;19(3):258-62.
4. 4. Tsafrir Z, Azem F, Hasson J, Solomon E, Almog B, Nagar H, et al. Risk factors, symptoms, and treatment of ovarian torsion in children: the twelve-year experience of one center. J Minim Invasive Gynecol. 2012;19(1):29-33.
5. 5. Beresewicz A, Maczewski M, Duda M. Effect of classic preconditioning and diazoxide on endothelial function and O2- and NO generation in the post-ischemic guinea-pig heart. Cardiovasc Res. 2004;63(1):118-29.
6. 6. Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am. 1992;72(1):65-83.
7. 7. Bozkurt S, Arikan DC, Kurutas EB, Sayar H, Okumus M, Coskun A, et al. Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation. J Pediatr Surg. 2012;47(9):1735-41.
8. 8. Feillet-Coudray C, Rock E, Coudray C, Grzelkowska K, Azais-Braesco V, Dardevet D, et al. Lipid peroxidation and antioxidant status in experimental diabetes. Clin Chim Acta. 1999;284(1):31-43.

9. 9. Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med. 2004;25(1-2):199-210.
10. 10. Güler MC, Tanyeli A, Ekinci Akdemir FN, Eraslan E, Özbek Şebin S, Güzel Erdoğan D, et al. An Overview of Ischemia-Reperfusion Injury: Review on Oxidative Stress and Inflammatory Response. Eurasian J Med. 2022;54(Suppl1):62-5.
11. 11. Zhao Y, Wu Z, Li D, Zhang Y, Guan G, editors. Intelligent computing methods used in acoustic emission and magnetic flux leakage detection of tank bottom. 2020 IEEE International Conference on Power, Intelligent Computing and Systems (ICPICS); 2020 28-30 July 2020.
12. 12. Nabavi SF, Tenore GC, Daglia M, Tundis R, Loizzo MR, Nabavi SM. The cellular protective effects of rosmarinic acid: from bench to bedside. Curr Neurovasc Res. 2015;12(1):98-105.
13. 13. Fonteles AA, de Souza CM, de Sousa Neves JC, Menezes AP, Santos do Carmo MR, Fernandes FD, et al. Rosmarinic acid prevents against memory deficits in ischemic mice. Behav Brain Res. 2016;297:91-103.
14. 14. Ferreira LG, Celotto AC, Capellini VK, Albuquerque AA, Nadai TR, Carvalho MT, et al. Is rosmarinic acid underestimated as an experimental cardiovascular drug? Acta Cir Bras. 2013;28 Suppl 1:83-7.
15. 15. Ramalho LN, Pasta AA, Terra VA, Augusto M, Sanches SC, Souza-Neto FP, et al. Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats. Food Chem Toxicol.2014;74:270-8.
16. 16. Güler MC, Tanyeli A, Erdoğan DG, Eraslan E, Çomaklı S, Polat E, et al. Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. Iran J Basic Med Sci. 2021;24(7):935-42.
17. 17. Topdağı Ö, Tanyeli A, Ekinci Akdemir FN, Eraslan E, Güler MC, Çomaklı S. Preventive effects of fraxin on ischemia/reperfusion-induced acute kidney injury in rats. Life Sci. 2020;242:117217.
18. 18. Güler MC, Tanyeli A, Eraslan E, Ekinci Akdemir FN, Nacar T, Topdağı Ö. Higenamine Decreased Oxidative Kidney Damage Induced By Ischemia Reperfusion in Rats. Kafkas Univ Vet Fak Derg. 2020;26(3):365-70.
19. 19. Güler MC, Tanyeli A, Eraslan E, Ekinci Akdemir FN. Role of 6-Shogaol Against Ovarian Torsion Detorsion-Induced Reproductive Organ Damage. NTMS. 2020;1(1):29-34.
20. 20. Güler MC, Tanyeli A. Role of Hyperoside on Ovarian Tissue Damage Created by Ovarian Torsion Detorsion. NTMS. 2020;1(1):1-5.
21. 21. Rahbardar MG, Amin B, Mehri S, Mirnajafi-Zadeh SJ, Hosseinzadeh H. Rosmarinic acid attenuates development and existing pain in a rat model of neuropathic pain: An evidence of anti-oxidative and anti-inflammatory effects. Phytomedicine. 2018;40:59-67.
22. 22. Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979;95(2):351-8.
23. 23. Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem. 1988;34(3):497-500.
24. 24. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982;78(3):206-9.
25. 25. Keith ES, Powers JJ. Effect of Phenolic Acids and Esters on Respiration and Reproduction of Bacteria in Urine. Appl Microbiol. 1965;13(3):308-13.
26. 26. Hibbard LT. Adnexal torsion. Am J Obstet Gynecol. 1985;152(4):456-61.
27. 27. Celik A, Ergun O, Aldemir H, Ozcan C, Ozok G, Erdener A, et al. Long-term results of conservative management of adnexal torsion in children. J Pediatr Surg. 2005;40(4):704-8.
28. 28. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000;190(3):255-66.
29. 29. Huchon C, Fauconnier A. Adnexal torsion: a literature review. Eur J Obstet Gynecol Reprod Biol. 2010;150(1):8-12.
30. 30. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med. 1985;312(3):159-63.
31. 31. Tok A, Sener E, Albayrak A, Cetin N, Polat B, Suleyman B, et al. Effect of mirtazapine on oxidative stress created in rat kidneys by ischemia-reperfusion. Ren Fail. 2012;34(1):103-10.
32. 32. Kusano C, Ferrari C. Total antioxidant capacity: A biomarker in biomedical and nutritional studies. JCMB. 2008;7:5407-12.
33. 33. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-11.
34. 34. Yazici S, Demirtas S, Guclu O, Karahan O, Yavuz C, Caliskan A, et al. Using oxidant and antioxidant levels to predict the duration of both acute peripheral and mesenteric ischemia. Perfusion. 2014;29(5):450-5.
35. 35. Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. NMCD. 2005;15(4):316-28.
36. 36. Jang HS, Kim J, Park YK, Park KM. Infiltrated macrophages contribute to recovery after ischemic injury but not to ischemic preconditioning in kidneys. Transplantation. 2008;85(3):447-55.
37. 37. Meister A. Glutathione deficiency produced by inhibition of its synthesis, and its reversal; applications in research and therapy. Pharmacol Ther. 1991;51(2):155-94.
38. 38. Psotova J, Kolar M, Sousek J, Svagera Z, Vicar J, Ulrichova J. Biological activities of Prunella vulgaris extract. PTR. 2003;17(9):1082-7.
39. 39. Swarup V, Ghosh J, Ghosh S, Saxena A, Basu A. Antiviral and Anti-Inflammatory Effects of Rosmarinic Acid in an Experimental Murine Model of Japanese Encephalitis. Antimicrob. Agents Chemother. 2007;51(9):3367-70.
40. 40. Yang EJ, Ku SK, Lee W, Lee S, Lee T, Song KS, et al. Barrier protective effects of rosmarinic acid on HMGB1-induced inflammatory responses in vitro and in vivo. J Cell Physiol. 2013;228(5):975-82.
41. 41. Ozturk H, Ozturk H, Terzi EH, Ozgen U, Duran A, Uygun I. Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats. JPMA J Pak Med Assoc. 2014;64(3):260-5.
42. 42. Luan H, Kan Z, Xu Y, Lv C, Jiang W. Rosmarinic acid protects against experimental diabetes with cerebral ischemia: relation to inflammation response. J Neuroinflammation. 2013;10:28.
43. 43. Bacanli M, Aydin S, Taner G, Goktas HG, Sahin T, Basaran AA, et al. Does rosmarinic acid treatment have protective role against sepsis-induced oxidative damage in Wistar Albino rats? Hum Exp Toxicol. 2016;35(8):877-86.
44. 44. Zhang Y, Chen X, Yang L, Zu Y, Lu Q. Effects of rosmarinic acid on liver and kidney antioxidant enzymes, lipid peroxidation and tissue ultrastructure in aging mice. Food Funct. 2015;6(3):927-31.
45. 45. Bayomy NA, Elbakary RH, Ibrahim MAA, Abdelaziz EZ. Effect of Lycopene and Rosmarinic Acid on Gentamicin Induced Renal Cortical Oxidative Stress, Apoptosis, and Autophagy in Adult Male Albino Rat. Anat Rec (Hoboken). 2017;300(6):1137-49.
46. 46. Shang AJ, Yang Y, Wang HY, Tao BZ, Wang J, Wang ZF, et al. Spinal cord injury effectively ameliorated by neuroprotective effects of rosmarinic acid. Nutr Neurosci. 2017;20(3):172-9.
47. 47. Değer U, Çavuş Y. Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models. Acta Cir Bras. 2020;35(3):e202000304.