Kolon Kanserinde Notch Yolağına Duyarlı Gen Ekspresyonlarının Araştırılması

Öz

Kolorektal kanser (CRC), tedaviye direnç ve metastaz nedeniyle kanserle ilişkili ölümlerin önde gelen nedenlerinden biridir. Bu çalışma, CRC’de Notch sinyal yolunun rolünü araştırarak sağlıklı CCD841-con hücreleri ile HCT116 kolorektal kanser hücreleri arasındaki yolakla ilişkili genlerin ekspresyonlarını karşılaştırmıştır. Yarı-kantitatif PCR analizi, HCT116 hücrelerinde GATA3 (4 kat), HES (5 kat) ve HEY (3 kat) genlerinin anlamlı şekilde arttığını, buna karşılık PTCRA ekspresyonunun 4 kat azaldığını ortaya koymuştur (p < 0.005, GAPDH’ye normalize edilmiştir). Bu sonuçlar, Notch sinyal yolunun CRC’de tümör progresyonuna katkıda bulunabileceğini göstermektedir. HES ve HEY genlerinin artmış ekspresyonu, epitel-mezenkimal geçiş (EMT) ve kanser kök hücre benzeri özelliklerle ilişkili olabilir. Genel olarak, bu çalışma Notch yolunun hedeflenmesinin CRC tedavisi için potansiyel bir strateji olabileceğini vurgulamakta ve tümör gelişimi ile direnç mekanizmalarındaki rolüne dair önemli bulgular sunmaktadır.

Anahtar Kelimeler

• Kolon kanseri
• Notch Sinyali
• overexpresyon

Investigation of Notch Pathway Responsive Gene Expressions In Colon Cancer

Abstract

Colorectal cancer (CRC) is a leading cause of cancer-related mortality, largely due to therapy resistance and metastasis. This study explored the role of Notch signaling in CRC by comparing the expression of pathway-associated genes between healthy CCD841-con cells and HCT116 colorectal cancer cells. Semi-quantitative PCR analysis revealed significant upregulation of GATA3 (4-fold), HES (5-fold), and HEY (3-fold) in HCT116 cells, while PTCRA expression decreased 4-fold (p < 0.005, normalized to GAPDH). These results suggest that Notch signaling contributes to tumor progression in CRC. The elevated expression of HES and HEY may be linked to epithelial-mesenchymal transition (EMT) and cancer stem cell-like traits. Overall, this study highlights the potential of targeting the Notch pathway in CRC therapy, offering insights into its role in tumor development and resistance mechanisms.

Keywords

• Colon cancer
• Notch signal
• overexpression

Kaynakça

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