Kültür Mantarından (Agaricus bisporus) İzole Edilen Kitosanın Hemostatik ve Antimikrobiyal Yara Örtüsünde Kullanımın Araştırılması

Öz

Bu çalışmada, Agaricus bisporus’tan kitosanı izole ederek elde edilen kitosanın yara örtüsünde kullanmak üzere antimikrobiyal ve hemostatik aktiviteleri araştırılması hedeflenmiştir. Bu çalışmada Agaricus bisporus kültür mantarına demineralizasyon işlemi ve ardından deproteinizasyon işlemi uygulayarak kitin elde edilmiştir. Elde edilen kitine deasetilasyon işlemi uygulayarak kitosan elde edilmiştir. Elde edilen kitosanın morfolojisi, kimyasal yapı ve deasetilasyon derecesi araştırılmıştır. Çalışma kapsamında antimikrobiyal edilen kitosanın Gram negatif ve Gram pozitif bakterilere karşı antimikrobiyal özelliği araştırılmıştır. Elde edilen kitosanın hemostatik aktivitesi araştırılmış ve kanamayı durdurmak için geçen süreyi belirlenmiştir. FE-SEM ile elde edilen morfoloji analiz sonuçlarına göre kitosanın yüzey morfolojisi amorf yapıya sahiptir. FT-IR spektroskopisi ile elde edilen kimyasal analiz sonuçlarında, kitosan için karakteristik pikler olan amid I bandının ve amid II'nin sırasıyla 1633 cm-1 ve 1558 cm-1 absorpsiyon bantları gözlemlenmiştir. Antimikrobiyal test sonuçlarına göre, Gram negatif ve Gram pozitif bakterilere karşı duyarlılık göstermiştir. Bununla birlikte elde edilen kitosan üzerine kan damlası bırakılarak pıhtı oluşum süresi 5 ve 14 saniye aralığında olduğu belirlenmiştir. Agaricus bisporus'tan elde edilen kitosan, antibakteriyel ve hemostatik özellikleriyle yara örtülerinin üretiminde ve biyomedikal uygulamalarda önemli bir bileşen olma potansiyeline sahiptir.

Anahtar Kelimeler

• Kitosan
• Kanama Durdurucu
• Antimikrobiyal
• Agaricus bisporus
• Yara Örtüsü.

The Use of Chitosan Extracted from Grown Mushrooms (Agaricus bisporus) in Hemostatic and Antibacterial Wound Dressing

Öz

The objective of this study was to examine the antimicrobial and hemostatic properties of chitosan derived from Agaricus bisporus, with the intention of utilizing it in wound dressings. This study involved the extraction of chitin from Agaricus bisporus mushrooms through demineralization and deproteinization techniques. The resulting chitin was then subjected to deacetylation to obtain chitosan. The morphology, chemical structure, and degree of deacetylation of the chitosan were examined. Furthermore, the antimicrobial efficacy of the chitosan was evaluated against both Gram-negative and Gram-positive bacteria. The study also investigated the hemostatic properties of the chitosan by determining the time required for bleeding cessation. Based on the morphology analysis using FE-SEM, it was found that the surface structure of the chitosan exhibited an amorphous nature. The chemical analysis using FT-IR spectroscopy revealed characteristic peaks for chitosan, specifically the absorption bands at 1633 cm-1 and 1558 cm-1, corresponding to the amide I and amide II bands, respectively. The antimicrobial testing demonstrated the chitosan's effectiveness against both Gram-negative and Gram-positive bacteria. Moreover, upon applying a drop of blood to the chitosan surface, it was observed that clot formation occurred within a timeframe ranging from 5 to 14 seconds. Chitosan obtained from Agaricus bisporus, with its antibacterial and hemostatic qualities, holds potential as a key component in the manufacture of wound dressings and biomedical applications.

Anahtar Kelimeler

• Chitosan
• Bleeding Stopper
• Antimicrobial
• Agaricus Bisporus
• Wound Dressing

Kaynakça

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