Rekreasyonel Olarak Aktif Bireylerde Nigella sativa Takviyesinin Egzersiz Sonrası Enflamasyon ve Kas Hasarı Üzerindeki Etkileri

Öz

Egzersiz kaynaklı kas hasarı (EIMD), kas lifi bozulmasına, iltihaplanmaya ve interlökin-6 (IL-6) ve kreatin kinaz (CK) gibi biyobelirteçlerin yükselmesine yol açar. Etkili iyileşme stratejileri, iltihabı azaltmak ve kas onarımını hızlandırmak için çok önemlidir. Timokinon açısından zengin olan Nigella sativa (NS), anti-inflamatuar ve antioksidan özelliklere sahiptir, ancak egzersiz sonrası IL-6 ve CK düzenlemesindeki rolü belirsizliğini korumaktadır. Bu çift kör, plasebo kontrollü çalışma, 40 rekreasyonel olarak aktif erkekte yüksek yoğunluklu eksantrik egzersizden sonra NS takviyesinin IL-6 ve CK seviyeleri üzerindeki etkilerini araştırmıştır. Katılımcılara NS (egzersizden önce 7 gün boyunca günde iki kez 500 mg) veya plasebo verildi. Eksantrik bir egzersiz protokolü uyguladılar (Nosaka & Miyama modeli), kan örnekleri başlangıçta (T0), egzersizden sonra (T1) ve egzersizden 24 saat sonra (T2) toplandı. IL-6 ve CK, ELISA ve otomatik bir biyokimyasal analiz cihazı kullanılarak analiz edildi ve veriler iki yönlü tekrarlanan ölçümler ANOVA kullanılarak işlendi. Egzersizden sonra, IL-6 ve CK seviyeleri her iki grupta da önemli ölçüde artarak inflamatuar bir yanıtı doğruladı. Ancak, IL-6 ve CK seviyeleri NS grubunda hem T1 hem de T2'de önemli ölçüde daha düşüktü (p < 0,001), bu da NS takviyesinin iltihabı ve kas hasarını etkili bir şekilde azalttığını gösteriyor. NS, BCAA'lar, kurkumin ve omega-3'e benzer şekilde doğal bir anti-inflamatuar ve kas koruyucu ajan olarak etki etme potansiyeline sahiptir. Ancak, optimum dozajı, uzun vadeli etkileri ve olası Diğer kurtarma stratejileriyle sinerjiler.

Anahtar Kelimeler

• Nigella Sativa
• IL-6
• kreatin kinaz
• kas hasarı
• inflamasyon
• spor iyileşmesi

Destekleyen Kurum

Universitas Negeri Surabaya

Etik Beyan

Bu çalışma etik standartlara uygun olarak yürütülmüş ve Airlangga Üniversitesi Halk Sağlığı Fakültesi Sağlık Araştırmaları Etik Komitesi'nden onay alınarak 10 Mayıs 2023 tarihli 104/EA/KEPK/2023 kimlik numarasıyla etik açıdan kabul edilebilir bulunmuştur

Teşekkür

Yazarlar, bu çalışmayı yürütmede araştırma hibesi için Spor Bilimi ve Sağlık Fakültesi, Universitas Negeri Surabaya'ya (UNESA) teşekkür etmek isterler. Yazarlar ayrıca, araştırma süreci boyunca değerli teknik yardım ve laboratuvar olanakları sağlayan ekibiyle Universitas Airlangga Halk Sağlığı Fakültesi'nin desteği ve iş birliğinden de minnettardırlar.

Effects of Nigella sativa Supplementation on Post-Exercise Inflammation and Muscle Damage in Recreationally Active Individuals

Öz

Exercise-induced muscle damage (EIMD) leads to muscle fiber disruption, inflammation, and elevated biomarkers such as interleukin-6 (IL-6) and creatine kinase (CK). Effective recovery strategies are crucial to mitigate inflammation and accelerate muscle repair. Nigella sativa (NS), rich in thymoquinone, possesses anti-inflammatory and antioxidant properties, but its role in post-exercise IL-6 and CK regulation remains unclear. This double-blind, placebo-controlled trial investigated the effects of NS supplementation on IL-6 and CK levels following high-intensity eccentric exercise in 40 recreationally active males. Participants received either NS (500 mg twice daily for 7 days pre-exercise or a placebo. They performed an eccentric exercise protocol (Nosaka & Miyama model), with blood samples collected at baseline (T0), post-exercise (T1), and 24 hours post-exercise (T2). IL-6 and CK were analyzed using ELISA and an automated biochemical analyzer, and data were processed using two-way repeated measures ANOVA. Post-exercise, IL-6 and CK levels increased significantly in both groups, confirming an inflammatory response. However, IL-6 and CK levels were significantly lower in the NS group at both T1 and T2 (p < 0.001), indicating that NS supplementation effectively reduced inflammation and muscle damage. NS has the potential to act as a natural anti-inflammatory and muscle-protective agent, similar to BCAAs, curcumin, and omega-3. However, further studies are needed to determine the optimal dosage, long-term effects, and possible synergies with other recovery strategies.

Anahtar Kelimeler

• Nigella Sativa
• IL-6
• Creatine Kinase
• Muscle Damage
• Inflammation
• Sports Recovery

Destekleyen Kurum

Universitas Negeri SUrabaya

Etik Beyan

This study followed ethical standards and received approval from the Health Research Ethics Committee, Faculty of Public Health, Airlangga University, and has been deemed ethically acceptable with the identity number: 104/EA/KEPK/2023, dated May 10, 2023

Teşekkür

The authors wish to acknowledge the Sports Science and Health Faculty, Universitas Negeri Surabaya (UNESA) for the research grant in conducting this work. The authors also gratefully acknowledge the support and collaboration of the Faculty of Public Health, Universitas Airlangga, whose team provided valuable technical assistance and laboratory facilities throughout the research process

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