Dodin fungusitinin Vicia faba L. bitkisinde total protein miktarı ve peroksidaz aktivitesi üzerine etkisi

Yıl 2016, , 627 - 633, 11.10.2016

Nurşen Çördük , Nihan Akıncı Nergis Kaya Gülru Yücel Cüneyt Akı

https://doi.org/10.16984/saufenbilder.22241

Cited By: 2

Öz

Bu araştırmada dodin fungusitinin Vicia faba L. fizyolojisi üzerindeki etkisi, total protein miktarı ve peroksidaz (POX) aktivitesi aracılığıyla değerlendirilmiştir. Bu doğrultuda, dört haftalık V. faba fideleri 0.04 mL/L, 0.08 mL/L ve 0.16 mL/L’lik dodin konsantrasyonları ile muamele edilmiştir. Bu işlemlerden sonra bitki örneklerinin yaprakları 24, 48 ve 72 saat ara ile hasat edilmiştir. Yapılan analizler sonucunda, artan dodin konsantrasyonu ve muamele süresiyle total protein miktarı kontrole göre önemli ölçüde azalırken, peroksidaz aktivitesi yükselmiştir. Elde edilen sonuçlar dodinin bitki savunma sistemini uyardığını göstermiştir.

 

Anahtar Kelimeler

1-dodesil guanidium asetat, enzim aktivitesi, Vicia faba, pestisit, total protein

Effects of dodine on total protein content and peroxidase activity in Vicia faba L.

Öz

This research was to evaluate the effects of dodine on the physiology of Vicia faba L. through total protein content and peroxidase (POX) activity. According to this, four weeks old seedlings were treated with 0.04 mL/L, 0.08 mL/L and 0.16 mL/L concentrations of dodine. Then, the leaves of plant samples were harvested with 24, 48 and 72 hours intervals. Our results have showed that while the total protein content were decreased significantly, POX activity was increased with the increased concentration of dodine and exposure time when compared with control seedlings. This research have shown that dodine can stimulate the plant defense system.

Anahtar Kelimeler

dodecylguanidinium acetate, enzyme activity, Vicia faba, pesticide, total protein

Kaynakça

• M. Gullino, P. Leroux and C. Smith, "Uses and challenges of novel compounds for plant disease control," Crop Prot, vol. 19, p. 1–11, 2000.
• X. Xia, Y. Huang and L. Wang, "Pesticides induced depression of photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus L.," Pestic Biochem Phys, vol. 86, p. 42–48, 2006.
• A. Paul, S. Nag and K. Sinha, "Cytological effect of Blitox on root mitosis of Allium cepa L.," IJSRP Int J Sci,, vol. 3, pp. 1-7, 2013.
• D. Ekinci and M. Şentürk, "Interactions of fungicides and pesticides with specific enzymes.," in Fungicides, O. Carisse, Ed., 2010.
• M. Dias, "Phytotoxicity: An overview of the physiological responses of plants exposed to fungicides," J Bot, 2012.
• T. Çelik, "Cytogenetic effects of some fungicide on barley root tip meristem cells," Pak J Biol Sci, vol. 9, pp. 2508-2511, 2006.
• "European Food Safety Authority Annual report on pesticide residues, vol 305," EFSA Scientific Report, Italy, 2009.
• N. Andrioli, S. Soloneski, M. Larramendy and M. Mudry, "Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro on meristematic cells of Allium cepa L.," Mutat Res-Gen Tox En, vol. 742, pp. 48-53, 1994.
• P. Sharma, A. B. Jha, R. S. Dubey and M. Pessarakli, "Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions.," J Bot, 2012.
• A. Salama, K. Osman and O. Omran, "Pesticide-induced oxidative damage: Possible in vitro protection by antioxidants," J Toxicol Environ Health Sci, vol. 5, pp. 79-85, 2013.
• K. Apel and H. Hirt, "Reactive oxygen species: Metabolism, oxidative stress, and signal transduction," Annu Rev Plant Biol, vol. 55, pp. 373-399, 2004.
• Y. Lee, S. Kim, J. Bang, H. Lee, S. Kwak and S. Kwon, "Enhanced tolerance to oxidative stress in transgenic tobacco plant expressing three antioxidant enzymes in chloroplasts," Plant Cell Rep, vol. 26, pp. 591-598, 2007.
• G. Noctor and C. Foyer, "Ascorbate and glutathione: keeping active oxygen under control," Annu Rev Plant Physiol Plant Mol Biol,, vol. 49, p. 249–279, 1998.
• K. Asada, "The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons," Annu Rev Plant Phys Plant Mol Biol, vol. 50, pp. 601-639, 1999.
• D. Vicuna, "The role of peroxidases in the development of plants and their responses to abiotic stresses, PhD dissertation," School of Food Science and Environmental Health, Dublin Institute of Technology, Dublin, 2005.
• J. Normanly, "Auxin metabolism," Physiol Plant, vol. 100, pp. 431-442, 1997.
• C. Huber, M. Preis, P. Harvey, S. Grosse, T. Letzeland and P. Schröder, "Emerging pollutants and plants – Metabolic activation of diclofenac by peroxidases," Chemosphere, vol. 146, pp. 435-441, 2016.
• A. Liszkay, B. Kenk and P. Schopfer, "Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth," Planta, vol. 214, no. 4, pp. 658-667, 2003.
• W. Fang and C. Kao, "Enhanced peroxidase activity in rice leaves in response to excess iron, copper and zinc," Plant Sci, vol. 158, pp. 71-76, 2000.
• A. Khan and N. Chaudhry, "Peroxidase activity (POX) in some cucurbits under the stress of mercury and lead," J Food Agric Environ, vol. 4, pp. 274-276, 2006.
• H. Teisseire and G. Vernet, "Effect of the fungicide Folpet on the activities of antioxidative enzymes in Duckweed (Lemna minör)," Pestic Biochem Physiol, vol. 69, pp. 112-117, 2001.
• Y. Wu and A. Tiedemann, "Impact of fungicides on active oxygen species and antioxidant enzymes in spring barley (Hordeum vulgare L.) exposed to ozone," Environ Pollut, vol. 116, pp. 37-42, 2002.
• Y. Mustapha, D. M. Réda, B. Houria and H. Nacira, "Effects of artea, a systemic fungicide, on the antioxidant system and the respiratory activity of durum wheat (Triticum durum L.)," Afr J Biotechnol, vol. 8, no. 17, pp. 4128-4130, 2009.
• G. Rabert, M. Rajasekar, P. Manivannan, R. Somasundaram and R. Panneerselvam, "Effect of triazole fungicide on biochemical and antioxidant enzymes activity in okra (Abelmoschus esculentus L.) plant under drought stress," Int J Agr Food Sci, vol. 3, pp. 100-107, 2013.
• H. Stevens, M. Calvan, K. Lee and B. Siegel, "Peroxidase activity as a screening parameter for salt stress in Brassica species," Phytochemistry, vol. 17, pp. 1521-1522, 1978.
• S. Lurie, E. Fallik, A. Handros and R. Shapier, "The possible involvement of peroxidase in resistance to Botrytis cinerea in heat treated tomato fruit," Physiol Mol Plant Path, vol. 50, p. 141–149, 1997.
• A. Caverzan, G. Passaia, S. Rosa, C. W. Ribeiro, F. Lazzarotto and M. Margis-Pinheiro, "Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection," Genet Mol Biol, vol. 35, p. 1011–1019, 2012.
• S. Wolff, A. Garner and R. Dean, "Free radicals, lipids and protein degradation," Trends Biochem Sci, vol. 11, pp. 27-31, 1986.
• M. Solomon, B. Belenghi, M. Delledonne and A. Levine, "The involvement of cysteine proteases and protease inhibitor genes in programmed cell death in plants," Plant Cell, vol. 11, pp. 431-444, 1999.
• M. Iqbal, "Vicia faba bioassay for environmental toxicity monitoring: A review.," Chemosphere, vol. 144, p. 785–802, 2016.
• R. Grene, "Oxidative Stress and Acclimation Mechanisms in Plants," The Arabidopsis Book, vol. Special revue, pp. 1-20, 2002.
• G. Fiskesjo, "The Allium cepa test in wastewater monitoring," Environ Toxic Water, vol. 8, pp. 291-298, 1993.
• M. Bradford, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding," Anal Biochem, vol. 72, pp. 248-254, 1976.
• J. Kanner and J. E. Kinsella, "Lipid deterioration initiated by phagocytic cells in muscle foods: B-Carotene destruction by a myeloperoxidase-hydrogen peroxide-halid system," J Agric Food Chem, vol. 31, pp. 370-376, 1983.
• T. Grune, P. Michel, N. Sitte, W. Eggert, H. Albrecht-Nebe, H. Esterbauer and W. Siems, "Increased levels of 4-hydroxynonenal modified proteins in plasma of children with autoimmune diseases," Free Radic Biol Med, vol. 23, p. 357–360, 1997.
• R. Pacifici, D. Salo and K. Davies, "Macroxyproteinase (M.O.P.): a 670 kDa proteinase complex that degrades oxidatively denatured proteins in red blood cells," Free Radical Bio Med, vol. 7, pp. 521-536, 1998.
• C. Person, J. Sambroski and F. Forysth, "The effect of BZI on detached wheat leaves," Can J Bot, vol. 180, pp. 1294-1295, 1957.
• D. Mishra and E. Waywood, "The effect of BZI and kinetin on the nicotinamide and nucleotide content of senescence wheat leaf," Can J Bot, vol. 46 , pp. 167-178, 1968.
• Z. Siddiqui and S. Ahmed, "Effects of systemic fungicides on protein, carbonhdyrate, amino acids and phenolic contents of susceptible (Mexipark) and resistant (Povan) varieties of Triticum aestivum L.," Turk J Bot, vol. 26, pp. 127-130, 2002.
• D. Guin, K. Sye, K. Dave and M. Gruebele, "Dodine as a transparent protein denaturant for circular dichroism and infrared studies," Protein Sci, vol. 25, pp. 1061-1068, 2016.
• H. Gelman, T. Perlova and M. Gruebele, "Dodine as a protein denaturant: The best of two worlds?," J Phys Chem B, , vol. 117, pp. 13090-13097, 2013.
• Z. Siddiqui and S. Ahmed, "Combined effects of pesticide on growth and nutritive composition of soybean plants," Pak J Bot, vol. 38, no. 3, pp. 721-733, 2006.
• N. Tort and B. Türkyilmaz, "Physiological effects of captan fungicide on pepper (Capsicum annuum L.) plant," Pak J Bot, vol. 6, pp. 2026-2029, 2003.
• A. Kiss, I. Varga, Z. Galbacs, T. Maria and A. Csikkel-Szolnoki, "Effect of age and magnesium supply on the free radical and antioxidant content of plants," Acta Biol Szeged, vol. 47, pp. 127-130 , 2003.
• C. H. Foyer and G. Noctor, "Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context," Plant Cell Environ, vol. 28, pp. 1056-1071, 2005.
• Y. Zhang, X. Zhang, C. Chen, M. Zhou and H. Wang, "Effects of fungicides JS399-19, azoxystrobin, tebuconazloe, and carbendazim on the physiological and biochemical indices and grain yield of winter wheat," Pestic Biochem Phys, vol. 98, pp. 151-157, 2010.
• A. Horotan and S. Oancea, "Effects of fungicide and acetylsalicylic acid treatments on the physiological and enzymatic activity in tomato (Lycopersicon esculentum Mill.)," Versita, vol. 17, pp. 13-26, 2013.
• D. Saha, S. Mandal and A. Saha, "Copper induced oxidative stress in tea (Camellia sinensis) leaves," J Environ Biol , vol. 33, p. 861–866, 2012.
• J. L. García-Hernández, H. Nolasco, E. Troyo-Diéguez, H. G. Jones and A. Ortega-Rubio, "The effects of selected insecticides on the superoxide dismutase activity in hot pepper plants (Capsicum annuum L. cv. Ancho San Luis)," Phyton Int J Exp Bot , pp. 67-74, 2001.
• J. L. García-Hernández, H. Nolasco, E. Troyo-Diéguez, B. Murillo-Amador, A. Flores-Hernández, I. Orona-Castillo and R. D. Valdez-Cepeda, "The effect of insecticides on peroxidase activity in hot pepper plants (Capsicum annum L.)," Rev Chapingo Ser Hortic, vol. 11, pp. 129-133, 2005.
• K. Mishra, E. Sanwal, P. K. Tandon and K. Gupta, "Metabolic effects of pesticide effluents on Nicotiana tabacum and Vignara diata plants," Int J Environ, vol. 4, pp. 87-94, 2015.
• N. Çördük, N. Akıncı, G. Yücel, N. Kaya and C. Akı, "Evaluation of Genotoxicity and Cytotoxicity of Dodine (1-dodecylguanidium acetate) by Allium Test," Fresen Environ Bull, vol. 24, pp. 4527-4531, 2015.