Spathiphyllum’un in vitro mikro çoğaltımı üzerine şeker konsantrasyonlarının etkileri

Yıl 2018, , 1015 - 1023, 01.06.2018

Mustafa Özzambak Emrah Zeybekoğlu , İzzet Gün Tuğba Kılıç

https://doi.org/10.16984/saufenbilder.348480

Cited By: 3

Öz

Fotoototrofik mikro çoğaltım, doku kültüründe gereksinim duyulan
karbonun karbondioksitten karşılanmasıdır. Bu yöntemin temeli besin ortamına
organik karbon kaynağı olarak şekerin ilave edilmemesi veya konsantrasyonunun
azaltılması koşuluna dayanmaktadır. Fotoototrofik mikro çoğaltım, doku
kültürünün en önemli sakıncaları olan vitrifikasyon, dış koşullara adaptasyon
güçlüğü, yoğun işgücü kullanımı, yüksek maliyet, kontaminasyon riski gibi
sorunları azaltabilmekte ya da ortadan kaldırabilmektedir. Bu çalışma ile Spathiphyllum wallisii’nin farklı şeker
konsantrasyonları içeren besin ortamlarındaki sürgün çoğaltma-köklendirme
kabiliyetinin in vitro fotoototrofik çoğaltıma uygunluk durumu belirlenmeye çalışılmıştır.
Sürgün çoğaltma denemesinde 8-10 mm uzunluğunda iki yapraklı in vitro sürgünler
MS + 0.5 mg/l BAP + 0.1 mg/l NAA ortamında; köklendirme denemesinde ise 25 mm
boyundaki in vitro sürgünler MS + 0.1 mg/l NAA ortamında kültüre alınmış olup;
her iki ortam da 0, 10, 20, 30 g/l konsantrasyonlarında şeker içermektedir.
Sürgün çoğaltma aşamasında, çoğalma oranı, kardeş sürgün adedi ve uzunluğu,
sürgün yaş ağırlığı, yaprak renk değerleri ve gelişme puanı bakımından şekerli
(10, 20, 30 g/l) ve şekersiz (0 g/l) ortamlar arasında istatistiksel olarak
önemli farklılıklar bulunmuştur. 10, 20, 30 g/l şeker konsantrasyonlarında
çoğalma sırasıyla 4.4, 4.6, 5.4 sürgün/eksp., 0 g/l şeker konsantrasyonunda 0.7
sürgün/eksp. olarak gerçekleşmiştir. Köklendirme aşamasında da ortamda şeker
bulunması incelenen özellikler üzerine pozitif yönde etki göstermiştir. 20, 30
g/l şeker konsantrasyonlarında köklenme oranı %100 olup; kök sayısı sırasıyla
16.1-8.8 adet/eksp., yaş kök ağırlığı ise 518.9-440.6 mg olarak belirlenmiştir.
Bu çalışma koşullarında fotoototofik mikro çoğaltmanın tam olarak
gerçekleşmediği; Spathipyllum’un in vitro sürgün çoğaltma ve köklendirilmesi
için besin ortamında 20 ya da 30 g/l konsantrasyonlarında şeker bulunması
gerektiği sonucuna varılmıştır.

Anahtar Kelimeler

Barış çiçeği, doku kültürü, fotoototrofik, besin ortamı, mikro sürgünler

Effects of sugar concentrations on in vitro micropropagation of spathiphyllum

Öz

Photoautotrophic micropropagation can be described as
a tissue culture technique in which, all required carbon is supplied from
carbon dioxide. Preparing the medium without or with low concentration of
sugar, which is a source of organic carbon, is the main condition. Decreasing
some problems as vitrification and contamination, lowering high input and labour
costs and improving the acclimatization capacity of in vitro plantlets are the
main advantages of photoautotrophic micropropagation. The present study was
carried out to determine the compatibility of spathiphyllum for in vitro
photoautotrophic micropropagation by proliferation and rooting ability of
microshoots on different sugar concentrations. In shoot multiplication trial, micro shoots in
8-10 mm length with two leafs were planted in MS + 0.5 mg/l BAP + 0.1 mg/l NAA
medium and in rooting experiment shoots in 25 mm length were transferred on MS
+ 0.1 mg/l NAA. Both media prepared with four different sugar concentrations (0, 10, 20, 30 g/l).
In multiplication stage, significant differences were found between medium with
sugar and without sugar at proliferation rate, new shoot number/exp. and
length, shoot fresh weight, leaf colour values and growth score parameters. 10, 20, 30 g/l
sugar concentrations gave the
highest proliferation rate with means of 4.4, 4.6, 5.4 shoots per explant and 0
g/l doses gave 0.7 shoot/exp. respectively. A positive relationship was found between
the increase in sugar concentration and rooting rate, root number, shoot fresh
weight, root dry weight. 100% rooting percentage, 16.1-8.8 root number per
explant 518.9-440.6 mg root fresh weight were verified for 20, 30 g/l sugar
respectively. In this study conditions, photoautotrophic
micropropagation was not be occurred exactly and the presence of 20 or 30
g/l sugar
concentrations was necessary for efficient in vitro proliferation and rooting
of Spathiphyllum.

Anahtar Kelimeler

Peace lily, tissue culture, photoautotrophic, nutrient medium, micro shoots, sucrose

Kaynakça

• [1] I. Iliev, A. Gajdosova, G. Libiakov and S.M. Jain, “Plant micropropagation,” in Plant Cell Culture: Essential Methods, M.R. Davey, P. Anthony, Ed. New York John Wiley & Sons, pp.1-23, 2010.
• [2] A. Gürel, Ş. Hayta, P. Nartop, M. Bayraktar, ve S. Fedakar, “Mikroçoğaltım,” Bitki Hücre ve Organ Kültürü Uygulamaları, Ege Üniversitesi Yayınları, no. 58, İzmir, s.95-102, 2013.
• [3] S.D. Purohit, J.A.T. da Silva and N.Habibi, “Current approaches for cheaper and better micropropagation techniques,” International journal of Plant Developmental Biology, vol. 5, no. 1, pp. 1-36, 2011.
• [4] R.L.M. Pierik, “In vitro culture of higher plants” ISBN. 90-2473531-9. Dordrecht- Netherlands, 1987.
• [5] T. Kozai, “Chapter I. Introduction,” in Photoautotrophic (Sugar Free Medium) Micropropagation as a New Micropropagation and Transplant Production System, T. Kozai, F. Afreen, S.M.A. Zobriyed, Ed. Springer, pp.19-31, 2005.
• [6] T.A. Thorpe, “Carbohydrate utilization and metabolism,” in Tissue Culture in Forestry J.M. Bonga, D.J. Durzan, Ed. Springer Dordrecht, pp. 325–368, 1982.
• [7] T. Ahmad, N. A. Abbasi, I. A. Hafız and A. Ali, “Comparison of sucrose and sorbitol as main carbon energy sources in micropropagation of peach rootstock,” GF-677 Pak. J. Bot., vol. 39, no.4, pp.1269-1275, 2007.
• [8] A.J. Ilczuk, K. Kubicec and K. Jacygrad, “The effect of carbon source in culture medium on micropropagation of common ninebark (P. opulifolius L.),” Mexim Diable D’or. Acta Sci. Pol. Horturum Cultus, vol. 12, no. 3, pp. 23-33, 2013.
• [9] T. Kozai and L. Kubota, “Concepts, definition, ventilation methods, advantages and disadvantage” in Photoautotrophic (Sugar Free Medium) Micropropagation as a New Micropropagation and Transplant Production System, T. Kozai, F. Afreen, S.M.A. Zobriyed, Ed. Springer, pp.19-31, 2005.
• [10] M. Cappellades, R. Lemeur and P. Debergh, “Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro,” Plant Cell, Tissue and Organ Culture, vol. 25, no. 1, pp. 21-26, 1991.
• [11] C. Hdider and Y. Desjardins, “Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets,” Plant Cell, Tissue and Organ Culture, vol. 1, no. 36, pp. 27-33, 1994.
• [12] Anonim, “Türk gıda kodeksi şeker tebliği,” Tebliğ No. 2006-40, Resmi Gazete No. 26268, 2006.
• [13] K.M. Swamy, K.M. Snipta, S. Balasubramanya and M. Anuradha, “Effect of different carbon sources on in vitro morphogenetic response of Patchouli (Pogostemon cahlin),” Journal of Phytology, vol. 2, no. 8, pp.11, 2010.
• [14] P. Demo and Kuria, “Table sugar as an alternative low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum L.),” African Journal of Biotechnology, vol. 7, no. 15, pp. 2578-2584, 2008.
• [15] T. Kozai and Q.T. Nguven, “Photoautotrophic micropropagation of woody and topical plants,” in Micropropagation of Woody Trees and Fruits, S.M. Jain, K. Ishii, Ed. Netherlands: pp. 757-781, 2003.
• [16] Y. Xio, T. Kozai, “Practical sugar-free micropropagation system using large vessels with forced ventilation,” in Transplant Production in the 21st Century, C. Kubota, C. Chun, Ed. Kluwer Acedemics, Netherlands, pp. 266-273, 2000.
• [17] N. Oral, “Spathiphyllum,” İç Mekan Süs Bitkileri, TAV Yayınları, No:14, s.168, Yalova, 1985.
• [18] T. Murashige and F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue cultures,” Physiol Plant, vol. 15, 473-497, 1962.
• [19] S.E. Doaa and M.S Mokhtar, “The impact of sucrose concentration on root growth and development in fig (Ficus carica L.) in vitro,” Assiut J. Agric. Sci., vol. 46, no. 6, pp. 67-75, 2015. [Online]. Available: from:https://www.researchgate.net/publication/299791909. [Accessed: 18-Aug-2017].
• [20] M. Zahara, A. Datta, P. Boonkorkaew and A. Mishra, “The effects of different media, sucrose concentrations and matural additivies on plantlet growth of Phalacnopsis hybrid ‘Pink’,” Brazilian. Archives of Biology and Technology, vol. 60, pp.1-15, 2017.
• [21] E.A. Jo, E.R.K. Tewari, E.J. Hahn and K.Y. Paek, “In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets,” Plant Cell Tiss Organ Culture, no. 96, pp. 307-315, 2009.
• [22] R. T. de Faria, F. N. Rodrigues, L. do V.R. Oliveira and C. Müller, “In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations,” Horticultura Brasileira, Brasília, v.22, n.4, p.780-783, 2004.
• [23] J.P.R. Martins, M. Pasqual, D.A. Martins and S.F. Riberia, “Effects of salts and sucrose concentration on in vitro propagation of Bilbergia zebrine,” Aust. J. of Crop Sci, vol. 9, no.1, pp. 85-91, 2015.
• [24] M. Kadota and Y. Niimi, “Influences of carbon sources and their concentrations on shoot proliferation and rooting of ‘Houi’ Japanese pear,” Hortscience vol.39, no.7, pp. 1681-1683, 2004.
• [25] N.S. Al-Khalifah, S. Hadi and F. Khan, “Influence of sucrose concentration on ın vitro growth of five rose cultivars,” Plant Tissue Culture, vol. 15, no. 1, pp. 43-49, 2005.
• [26] E.A. Morfeine, “Effect of sucrose and glucose concentrations on micropropagation of Musa sp.cv. Grand Naine,” Journal of Applied and Industrial Sciences, vol. 2, no. 2, pp. 58-62, 2014.
• [27] R. Lembrechts, V. Verdoodt, M.P. De Proft and J. Ceusters, “Influence of sucrose concentration on photosynthetıc performance of Guzmanıa 'Hılda',” In Vıtro. Acta Hortic. vol. 1083, pp.403-408, 2015.