Effects of Plant Growth Promoting Rhizobacteria (PGPR) on Physiological Parameters Against Salinity in Apple Cultivar “Fuji”

Yıl 2020, , 281 - 286, 01.04.2020

Şeyma Arıkan , Lütfi Pırlak

https://doi.org/10.16984/saufenbilder.469522

Cited By: 4

Öz

The present study was conducted with the cultivar ‘Fuji’ grafted on M9 rootstock in both 2014 and 2016 years. The effect of PGPR (Bacillus subtilis EY2, Bacillus atrophaeus EY6, Bacillus spharicus GC subgroup B EY30, Staphylococcus kloosii EY37 and Kocuria erythromyxa EY43) were investigated under salt stress conditions. PGPR’s effects were tested on leaf relative water content (LRWC), membrane permeability, stomatal conductivity, photosynthetic activity and chlorophyll content (by SPAD-502). The saplings were grown in pots filled 2:1:1 peat: perlite: sand. Salinity was obtained by NaCl: Na2SO4: CaCl2: MgSO4 (7:9:3:1) solution. The solution was applied twice a week with irrigation during the growing period. When the salinity reached 2.5-3.0 dScm-1, the solution application was ended. All bacteria treatments significantly reduced the physiological damage of leaves compared with the salt treatment in both two years. The LRWC range from 13.33 % (salt treatment) to 26.76 % (control). The best result of bacteria treatment was measured in EY43 with 23.93 % LRWC. The highest rate of membrane permeability was found in salt treatment (30.35 %). The stomatal conductivity was decreased in the salt application (154.35 mmol m-2s-1) unlike EY43 treatment (234.44 mmol m-2s-1). Similarly, EY43 treatment significantly increased photosynthetic activity (15.24 µmol CO2 m-2s-1) compared with the salt treatment (8.22 µmol CO2 m-2s-1). As a result, bacteria strains had been ameliorative of the deleterious effects under salt stress on “Fuji”.

Anahtar Kelimeler

Salt Stress, Apple, Fuji, PGPR

Kaynakça

• [1] A. Alizadeh, X. Xlilova, and A. Eivazi, "Biochemical Response of Apple Dwarf Rootstock to Salinity Stress," Tech. J. Eng. Appl. Sci., vol. 1, pp. 118-124, 2011.
• [2] Anonymous, "www.fao.org Access date: 2016," 2008.
• [3] E. V. Maas and G. Hoffman, "Crop salt tolerance\-current assessment," Journal of the irrigation and drainage division, vol. 103, no. 2, pp. 115-134, 1977.
• [4] M. Ashraf, S. Hasnain, O. Berge, and T. Mahmood, "Inoculating wheat seedlings with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress," Biology and Fertility of Soils, vol. 40, no. 3, pp. 157-162, 2004.
• [5] H. Karlidag, A. Esitken, E. Yildirim, M. F. Donmez, and M. Turan, "Effects of plant growth promoting bacteria on yield, growth, leaf water content, membrane permeability, and ionic composition of strawberry under saline conditions," Journal of plant nutrition, vol. 34, no. 1, pp. 34-45, 2011.
• [6] H. Antoun and D. Prévost, "Ecology of plant growth promoting rhizobacteria," in PGPR: Biocontrol and biofertilization: Springer, 2005, pp. 1-38.
• [7] Z. A. Zahir, M. Arshad, and W. T. Frankenberger, "Plant growth promoting rhizobacteria: applications and perspectives in agriculture," Advances in Agronomy, vol. 81, pp. 97-168, 2003.
• [8] C. S. Jacobsen, "Plant protection and rhizosphere colonization of barley by seed inoculated herbicide degrading Burkholderia (Pseudomonas) cepacia DBO1 (pRO101) in 2, 4-D contaminated soil," Plant and Soil, vol. 189, no. 1, pp. 139-144, 1997.
• [9] Y. Bashan and L. de Bashan, "Plant growth-promoting," Encyclopedia of soils in the environment, vol. 1, pp. 103-115, 2005.
• [10] H. Karlidag, E. Yildirim, M. Turan, M. Pehluvan, and F. Donmez, "Plant growth-promoting rhizobacteria mitigate deleterious effects of salt stress on strawberry plants (Fragaria× ananassa)," HortScience, vol. 48, no. 5, pp. 563-567, 2013.
• [11] Y. Bashan, M. Moreno, and E. Troyo, "Growth promotion of the seawater-irrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirillum spp," Biology and Fertility of Soils, vol. 32, no. 4, pp. 265-272, 2000.
• [12] P. M. Hasegawa, R. A. Bressan, J.-K. Zhu, and H. J. Bohnert, "Plant cellular and molecular responses to high salinity," Annual review of plant biology, vol. 51, no. 1, pp. 463-499, 2000.
• [13] R. Munns, "Comparative physiology of salt and water stress," Plant, cell & environment, vol. 25, no. 2, pp. 239-250, 2002.
• [14] W.-Y. Kao, T.-T. Tsai, and C.-N. Shih, "Photosynthetic gas exchange and chlorophyll a fluorescence of three wild soybean species in response to NaCl treatments," Photosynthetica, vol. 41, no. 3, pp. 415-419, 2003.
• [15] M. Ashraf and M. Shahbaz, "Assessment of genotypic variation in salt tolerance of early CIMMYT hexaploid wheat germplasm using photosynthetic capacity and water relations as selection criteria," Photosynthetica, vol. 41, no. 2, pp. 273-280, 2003.
• [16] S. Kumari, A. Vaishnav, S. Jain, A. Varma, and D. K. Choudhary, "Bacterial-mediated induction of systemic tolerance to salinity with expression of stress alleviating enzymes in soybean (Glycine max L. Merrill)," Journal of Plant Growth Regulation, vol. 34, no. 3, pp. 558-573, 2015.
• [17] E. Brugnoli and O. Björkman, "Growth of cotton under continuous salinity stress: influence on allocation pattern, stomatal and non-stomatal components of photosynthesis and dissipation of excess light energy," Planta, vol. 187, no. 3, pp. 335-347, 1992.
• [18] H. Han and K. Lee, "Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity," Res J Agric Biol Sci, vol. 1, no. 3, pp. 210-215, 2005.
• [19] A. Golpayegani and H. G. Tilebeni, "Effect of biological fertilizers on biochemical and physiological parameters of basil (Ociumum basilicm L.) medicine plant," Am Eurasian J Agric Environ Sci, vol. 11, pp. 411-416, 2011.
• [20] R. Porcel, Á. M. Zamarreño, J. M. García-Mina, and R. Aroca, "Involvement of plant endogenous ABA in Bacillus megaterium PGPR activity in tomato plants," BMC plant biology, vol. 14, no. 36, pp. 1-12, 2014.
• [21] S. Mahmood et al., "Plant Growth Promoting Rhizobacteria and Silicon Synergistically Enhance Salinity Tolerance of Mung Bean," Frontiers in Plant Science, vol. 7, p. 876, 2016.
• [22] P. S. Shukla, P. K. Agarwal, and B. Jha, "Improved salinity tolerance of Arachis hypogaea (L.) by the interaction of halotolerant plant-growth-promoting rhizobacteria," Journal of plant growth regulation, vol. 31, no. 2, pp. 195-206, 2012.
• [23] Ş. Arikan, M. İpek, and L. Pirlak, "Effect Of Some Plant Growth Promoting Rhizobacteria (Pgpr) On Growth, Leaf Water Content And Membrane Permeability Of Two Citrus Rootstock Under Salt Stress Condition," presented at the VII International Scientific Agriculture Symposium, Jahorina, Bosnia and Herzegovina, October 06 - 09, 2016 2016.