An in Vivo Study for the Use of Lupinus Albus (Fabaceae) in Drosophila Melanogaster Diet

Abstract

Based on the oral chronic toxicity studies in humans, the daily intake of Lupinus albus L. (termiye, white lupine) with diet is recommended to be about 0.02%. The study was designed to investigate the use of lupine shells in nutrition. Dried L. albus shell was added to the artificial diet of the model organism (Drosophila melanogaster) and then lipid peroxidation, antioxidant enzyme activity, total oxidation, and total antioxidant activity were determined in the tissues obtained from the third larval phase. Changes that occurred in the larval midgut cells were examined microscopically. As the amount of shell consumption increased, malondialdehyde concentration (0.33 - 0.09 ± 0.71 nmol/ mg protein) and glutathione S transferase activity (19.91 - 14.06 ± 0.04 nmol/ mg protein/ dk) decreased statistically compared to the control. In addition to this, larval total oxidation level and total antioxidant activity also decreased (P < 0.05). No damage was detected in the larval midgut epithelial cells.

Keywords

• Drosophila melanogaster
• Lipid peroxidation
• Lupinus albus
• Oxidative status
• Total antioxidant activity

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