Detection Of Genotoxicity Induced By Heavy Metal Ions And Gamma Radiation Using Micronucleus Assay In Mice: Pathological Evaluation

Yıl 2010, Cilt: 14 Sayı: 1, 1 - 8, 01.06.2010

Kültiğin Çavuşoğlu Emine Yalçın Oğuz Kul Abdullah Yılmaz Kürşad Yapar

Öz

-

Anahtar Kelimeler

Detection Of Genotoxicity Induced By Heavy Metal Ions And Gamma Radiation Using Micronucleus Assay In Mice: Pathological EvaluationKültiğin Çavuşoğlu1, Emine Yalçın1, Oğuz Kul2, Abdullah Yılmaz3, Kürşad Yapar41Department Of Biology, Faculty Of Science A

Farelerde Mikronukleus Testi Kullanılarak Gama Radyasyonu Ve Ağır Metal İyonları Tarafindan Teşvik Edilen Genotoksisitenin Belirlenmesi: Patolojik Değerlendirmeli

Öz

Micronuclei (MN) test is used as markers of radiosensitivity or chemosensitivity. In present study, it was investigated the frequency of MN in erythrocytes and body weight gain in 80 Mus musculus var. albinos exposed to 10 Gy gamma (γ) radiation and heavy metal ions. For this aim, it was used MN assay as an indicator of genotoxicity induced by γradiation and heavy metal toxication. The animals were divided into four groups: control, radiation, Hg and Pb treatment groups. They were treated with three dose levels (10, 15 and 20 µg/mL) of Hg and Pb metal ions and 10 Gy γradiaton was applied twice during 14 days. The initial and final weights of all mice were determined by sensitive balance in order to investigate the effect of heavy metal ions and radiation on the weight gain of mice. As a result, the frequency of MN was higher in the Hg, Pb and γradiation treated animals than animals in control group. Besides, MN frequency was higher in mice exposed to γradiation than in Hg and Pb treated mice, and differences was statistically significant (p

Anahtar Kelimeler

Farelerde Mikronukleus Testi Kullanılarak Gama Radyasyonu Ve Ağır Metal İyonları Tarafindan Teşvik Edilen Genotoksisitenin Belirlenmesi: Patolojik DeğerlendirmeliKültiğin Çavuşoğlu1, Emine Yalçın1, Oğuz Kul2, Abdullah Yılmaz3, Kürşad Yapar41Giresun Üniv

Kaynakça

• Botkin, D. and Keller, E., Environmental Science: Earth as a Living planet, John Wiley and Sons, Inc., New York, (1995).
• Al–Sabti, K., Frequency of chromosomal aberrations in the rainbow trout (Oncorhynchus mykiss) exposed to five pollutants, J Fish Biol, 26, 13–19 (1999).
• Wisconsin Department of Health and Family Services Division of Public Health with funds from the Agency for Toxic Substances and Disease Registry. Public Health Service, US, (2000).
• Henry, J. R., An overview of the phytoremediation of lead and mercury. National Network of Environmental Management Protection Agency, Washington D.C., (2000). (NNEMS). Environmental
• Ulupinar, M. and Okumus, I., Detection of mutagenic- carcinogenic pollutants in aquatic systems using cytogenetic methods in fish, Turk J Zool, 26, 141–148 (2002).
• Bellinger, D. and Sloman, J., Low-Level Lead Exposure and Children's Cognitive Function in the preschool Years, Pediatrics, 87, 219–227 (1991).
• Maes, A., Verschaeve, L., and Arrova, A., In Vitro Cytogenetic Effects of 2450 MHz Wawes on Human Peripheral Blood Lymphocytes, Bioelectromagnetics, 14, 495–501 (1993).
• Kim, Y. A., Fomenko, F. S. and Agafonova, T. A., Effect of microwawe radiation on different structural levels of erythrocyte membranes, Bioelectromagnetics, 6, 305–312 (1985).
• Antonopoulos, A., Eisenbrandt, H. and Obe, G., Effects of high-frequency electromagnetic fields on human lymphocytes in vitro, Mutat Res, 395, 209–214 (1997).
• Kumari, R., Chaugule, A. and Goyal, P. K., Karyoanomalic frequency during radiation therapy, J Cancer Res, 1 (3), 187–190 (2005).
• Heddle, I. A., Hite, M., Kirkhart, B., The induction of micronuclei as a measure of genotoxicity, A report of the US Environmental Protection Agency Gene-Tox Program, Mutat Res, 123: 61–118 (1983).
• Bonassi, S., Neri, M. and Lando, C., Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus Project, Mutat Res, 543, 155–166 (2003).
• Hitoshi, I., Ying, T. and Toru, Y., Influence of gender, age and lifestyle factors on micronuclei frequency in healthy Japanese populations, J Occup Health, 45, 179– 181 (2003).
• Wilson, E. B., The Cell in Developrnentand Heredity, Macmillan, New York, 1232 (1925).
• Evans, H. J., Neary, G. J. and Williams, F. S., The relative biological efficiency of single doses of fast neutrons and gamma-rays on Vicia faba roots and the effect of oxygen. Part II. Chromosome damage: the production of micronuclei, Int J Radial Biol, 3, 216–229 (1959).
• Heddle, J. A., A rapid in vivo test for chromosomal damage, Mutat Res, 18, 187–190 (1973).
• Thierens, H., Aousalah, B. and Vral, A., A chromosomal radiosensitivity study of a population of radiation workers using the micronucleus assay, Int J Low Radiat, 1, 102–112 (2003).
• Natarajan, A. T., Tucker, J. D., Sasaki, M. S., Monitoring cytogenetic damage in vivo. In: Tardiff R G, Lohman P H M, Wogan G N (ed.): Methods to Assess DNA Damage and Repair, Interspecies Comparisons, Wiley, Chichester, 231–254 (1994).
• Wuttke, K., Streffer, C. and Muller, W. U., Micronuclei in lymphocytes of children from the vicinity of Chernobyl before and after therapy for thyroid cancer, Int J Radiat Biol, 69, 259–269 (1996).
• Fenench, M., Important variables that influence base- line micronucleus frequency in cytokinsis-blocked lymphocytes as biomarker for DNA damage in human populations, Mutat Res, 404, 155–165 (1998).
• Sener, G., Jahovic, N. and Tosun, O., Melatonin ameliorates ionizing radiation-induced oxidative organ damage in rats, Life Sci, 74, 563–572 (2003).
• Cole, R. J., Taylor, N. and Cole, J., Short term tests for transplacentally active carcinogens. I. Micronucleus formation in fetal and maternal mouse erythroblasts, Mutat Res, 80, 141–57 (1981).
• Toxicological Principles for the Safety Assessment of Food Ingredients Redbook. Mammalian Erythrocyte Micronucleus Test, US (2000).
• Korkmaz, M. and Colak, A., The cytogenetics of NPYR on mice, Turk J Biol, 24, 1–12 (2000).
• Berces, J., Otos, M. and Szirmai, S., Using the Micronucleus Assay to Detect Genotoxic Effects of Metal Supllements, 101, 11-13 (1993). Health Perspectives
• Kennedy, A. R. and Szuhaj, B. F., Method of inhibiting radiation induced weight and hair loss, The Trustees of the University of Pennsylvania, Central Soya Company Inc., USA (1994).
• Oshomah, A. H., The Effect Of Living In Polluted Environment: A Case Study Of Albino Mice Subjected To Lead Assimilation At Three Different Sources, The Internet Journal of Toxicology, 3, 1-5 (2006).
• Kim, K. R., Son, E. W. and Hee–Um, S., Immune alterations in mice exposed to the herbicide simazine, J Toxicol Env Health Part A 66 (12), 1159–1173 (2003).
• NTP technical report on the carcinogenesis bioassay of Allyl Isothiocyanate (Cas No. 57-06-7) In F344/N Ats And B6c3f1 mice (Gavage study), Department of Health and Human Services Public Health Service National Institutes of Health, US (1982).