Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry
Öz
The
quantitative analysis of ciprofloxacin in an ophthalmic solution was performed
by UV absorption spectrophotometry and first derivative spectrophotometry. The
UV absorption spectra of standard series and samples containing ciprofloxacin
were recorded. Then, the derivative of UV absorption spectra was obtained for
the derivative spectrophotometric analysis of the related drug. Linear regression equations in the linear concentration
range of 3.0-28.0 µg/mL for analysis of ciprofloxacin were obtained by using
direct absorbance measurement at 278.9 nm and using the dA/dλ values at 283.2
nm. The recovery assay results were found between 97.8 to 102.0 %. It was
observed that the limits of detection and quantification were 0.74 and 2.74
μg/mL for UV absorption spectrophotometry and 0.60 and 2.00 μg/mL for first
derivative spectrophotometry, respectively. Analysis results indicated that the
proposed methods were precise, accurate and reliable for the determination of
ciprofloxacin in commercial samples.
Anahtar Kelimeler
Ciprofloxacin Spectrophotometry Derivative spectrophotometry Ophthalmic solution Quantitative analysis
Kaynakça
- K. R. Wilhelmus, R. L. Abshire, and B. A. Schlech, "Influence of fluoroquinolone susceptibility on the therapeutic response of fluoroquinolone-treated bacterial keratitis," Arch Ophthalmol., 121 (9), 1229-1233, 2003.
- E. C. L. Cazedey and H. R. N. Salgado, "Spectrophotometric determination of ciprofloxacin hydrochloride in ophthalmic solution," Advances in Analytical Chemistry, 2 (6), 74-79, 2012.
- A. W. Qassim, "Spectrophotometric Determination of Ciprofloxacin Hydrochloride in Pharmaceutical Formulation Ciproxin," IJASTR, 3, 135, 2015.
- S.-S. Wu, C.-Y. Chein, and Y.-H. Wen, "Analysis of ciprofloxacin by a simple high-performance liquid chromatography method," J. Chromatogr. Sci., 46(6), 490-495, 2008.
- K. Sowinski and M. Kays, "Determination of ciprofloxacin concentrations in human serum and urine by HPLC with ultraviolet and fluorescence detection," J. Clin. Pharm. Ther, 29(4), 381-387, 2004.
- Z. Vybiralova, M. Nobilis, J. Zoulova, J. Kvetina, and P. Petr, "High-performance liquid chromatographic determination of ciprofloxacin in plasma samples," J. Pharm. Biomed. Anal., 37 (5), 851-858, 2005.
- S. Imre, M. T. Dogaru, C. Vari, T. Muntean, and L. Kelemen, "Validation of an HPLC method for the determination of ciprofloxacin in human plasma," J. Pharm. Biomed. Anal., 33(1), 125-130, 2003.
- I. F. Al-Momani, A. T. Haj-Hussein, and A. N. Tahtamouni, "Flow injection spectrophotometric and chromatographic determination of ciprofloxacin and norfloxacin in pharmaceutical formulations," J. Flow Injection Anal., 25, 151-155, 2008.
- C. Fierens, S. Hillaert, and W. Van den Bossche, "The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis," J. Pharm. Biomed. Anal., 22 (5), 763-772, 2000.
- L. Tong, P. Li, Y. Wang, and K. Zhu, "Analysis of veterinary antibiotic residues in swine wastewater and environmental water samples using optimized SPE-LC/MS/MS," Chemosphere, 74 (8), 1090-1097, 2009.
- S. Zhang and S. Wei, "Electrochemical determination of ciprofloxacin based on the enhancement effect of sodium dodecyl benzene sulfonate," Bull. Korean Chem. Soc., 28 (4), 543-546, 2007.
- L. Fotouhi and M. Alahyari, "Electrochemical behavior and analytical application of ciprofloxacin using a multi-walled nanotube composite film-glassy carbon electrode," Colloids Surf B: Biointerfaces, 81 (1), 110-114, 2010.
- P. Gayen and B. P. Chaplin, "Selective electrochemical detection of ciprofloxacin with a porous nafion/multiwalled carbon nanotube composite film electrode," ACS Appl. Mater. Interfaces, 8 (3), 1615-1626, 2016.
UV Absorbans Spektrofotometrisi ve Türev Spektrofotometrisi Kullanılarak Oftalmik Çözeltilerde Siprofloksasinin Kantitatif Tayini
Öz
Bu
çalışmada oftalmik çözeltide siprofloksasinin kantitatif tayini için UV
absorbans spektrofotometrisi ve türev spektrofotometrisi kullanılmıştır.
Siprofloksasin içeren standart serinin ve numunelerin UV absorbans spektrumları
kaydedilmiştir. Daha sonra türev spektrofotometrisi için, UV absorbans spektrumunun
birinci türevi alınmıştır. 3.0-28.0 µg/mL doğrusal çalışma aralığında olmak
üzere, 278.9 nm’deki absorbans değerleri, ve 283.2 nm’deki dA/dλ değerleri
kullanılarak lineer regresyon eşitlikleri elde edilmiştir. Geri kazanım
çalışmaları sonuçları % 97.8 ile % 102.0
arasında hesaplanmıştır. Yakalama ve tayin sınır değerleri, UV absorbans
spektrofotometrisi için 0.74 µg/mL ve 2.74
µg/mL; türev spektrofotometrisi için 0.60 µg/mL
ve 2.00 µg/mL olarak hesaplanmıştır. Sonuçlar, siprofloksasinin ticari örneklerden
analizi için önerilen yöntemlerin kesin, doğru ve güvenilir olduğunu
göstermiştir.
Anahtar Kelimeler
Siprofloksasin Spektrofotometri Türev spektrofotometrisi Oftalmik çözelti Kantitatif analiz
Kaynakça
- K. R. Wilhelmus, R. L. Abshire, and B. A. Schlech, "Influence of fluoroquinolone susceptibility on the therapeutic response of fluoroquinolone-treated bacterial keratitis," Arch Ophthalmol., 121 (9), 1229-1233, 2003.
- E. C. L. Cazedey and H. R. N. Salgado, "Spectrophotometric determination of ciprofloxacin hydrochloride in ophthalmic solution," Advances in Analytical Chemistry, 2 (6), 74-79, 2012.
- A. W. Qassim, "Spectrophotometric Determination of Ciprofloxacin Hydrochloride in Pharmaceutical Formulation Ciproxin," IJASTR, 3, 135, 2015.
- S.-S. Wu, C.-Y. Chein, and Y.-H. Wen, "Analysis of ciprofloxacin by a simple high-performance liquid chromatography method," J. Chromatogr. Sci., 46(6), 490-495, 2008.
- K. Sowinski and M. Kays, "Determination of ciprofloxacin concentrations in human serum and urine by HPLC with ultraviolet and fluorescence detection," J. Clin. Pharm. Ther, 29(4), 381-387, 2004.
- Z. Vybiralova, M. Nobilis, J. Zoulova, J. Kvetina, and P. Petr, "High-performance liquid chromatographic determination of ciprofloxacin in plasma samples," J. Pharm. Biomed. Anal., 37 (5), 851-858, 2005.
- S. Imre, M. T. Dogaru, C. Vari, T. Muntean, and L. Kelemen, "Validation of an HPLC method for the determination of ciprofloxacin in human plasma," J. Pharm. Biomed. Anal., 33(1), 125-130, 2003.
- I. F. Al-Momani, A. T. Haj-Hussein, and A. N. Tahtamouni, "Flow injection spectrophotometric and chromatographic determination of ciprofloxacin and norfloxacin in pharmaceutical formulations," J. Flow Injection Anal., 25, 151-155, 2008.
- C. Fierens, S. Hillaert, and W. Van den Bossche, "The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis," J. Pharm. Biomed. Anal., 22 (5), 763-772, 2000.
- L. Tong, P. Li, Y. Wang, and K. Zhu, "Analysis of veterinary antibiotic residues in swine wastewater and environmental water samples using optimized SPE-LC/MS/MS," Chemosphere, 74 (8), 1090-1097, 2009.
- S. Zhang and S. Wei, "Electrochemical determination of ciprofloxacin based on the enhancement effect of sodium dodecyl benzene sulfonate," Bull. Korean Chem. Soc., 28 (4), 543-546, 2007.
- L. Fotouhi and M. Alahyari, "Electrochemical behavior and analytical application of ciprofloxacin using a multi-walled nanotube composite film-glassy carbon electrode," Colloids Surf B: Biointerfaces, 81 (1), 110-114, 2010.
- P. Gayen and B. P. Chaplin, "Selective electrochemical detection of ciprofloxacin with a porous nafion/multiwalled carbon nanotube composite film electrode," ACS Appl. Mater. Interfaces, 8 (3), 1615-1626, 2016.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Kimya Mühendisliği |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Mayıs 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 14 Sayı: 1 |
