Yıl 2019, Cilt 14 , Sayı 1, Sayfalar 71 - 76 2019-05-31

UV Absorbans Spektrofotometrisi ve Türev Spektrofotometrisi Kullanılarak Oftalmik Çözeltilerde Siprofloksasinin Kantitatif Tayini
Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry

Saadet DERMİŞ [1] , Sümeyye KILIÇ [2] , Zehra Ceren ERTEKİN [3] , Erdal DİNÇ [4]


Bu çalışmada oftalmik çözeltide siprofloksasinin kantitatif tayini için UV absorbans spektrofotometrisi ve türev spektrofotometrisi kullanılmıştır. Siprofloksasin içeren standart serinin ve numunelerin UV absorbans spektrumları kaydedilmiştir. Daha sonra türev spektrofotometrisi için, UV absorbans spektrumunun birinci türevi alınmıştır. 3.0-28.0 µg/mL doğrusal çalışma aralığında olmak üzere, 278.9 nm’deki absorbans değerleri, ve 283.2 nm’deki dA/dλ değerleri kullanılarak lineer regresyon eşitlikleri elde edilmiştir. Geri kazanım çalışmaları sonuçları % 97.8 ile % 102.0 arasında hesaplanmıştır. Yakalama ve tayin sınır değerleri, UV absorbans spektrofotometrisi için 0.74 µg/mL ve 2.74 µg/mL; türev spektrofotometrisi için 0.60 µg/mL ve 2.00 µg/mL olarak hesaplanmıştır. Sonuçlar, siprofloksasinin ticari örneklerden analizi için önerilen yöntemlerin kesin, doğru ve güvenilir olduğunu göstermiştir. 

The quantitative analysis of ciprofloxacin in an ophthalmic solution was performed by UV absorption spectrophotometry and first derivative spectrophotometry. The UV absorption spectra of standard series and samples containing ciprofloxacin were recorded. Then, the derivative of UV absorption spectra was obtained for the derivative spectrophotometric analysis of the related drug.  Linear regression equations in the linear concentration range of 3.0-28.0 µg/mL for analysis of ciprofloxacin were obtained by using direct absorbance measurement at 278.9 nm and using the dA/dλ values at 283.2 nm. The recovery assay results were found between 97.8 to 102.0 %. It was observed that the limits of detection and quantification were 0.74 and 2.74 μg/mL for UV absorption spectrophotometry and 0.60 and 2.00 μg/mL for first derivative spectrophotometry, respectively. Analysis results indicated that the proposed methods were precise, accurate and reliable for the determination of ciprofloxacin in commercial samples.

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Birincil Dil en
Konular Mühendislik, Kimya
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-5459-5818
Yazar: Saadet DERMİŞ (Sorumlu Yazar)
Kurum: ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Sümeyye KILIÇ
Kurum: ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-1549-6024
Yazar: Zehra Ceren ERTEKİN
Kurum: ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ

Orcid: 0000-0001-6326-1441
Yazar: Erdal DİNÇ
Kurum: ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ

Tarihler

Yayımlanma Tarihi : 31 Mayıs 2019

Bibtex @araştırma makalesi { sdufeffd458082, journal = {Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi}, issn = {}, eissn = {1306-7575}, address = {}, publisher = {Süleyman Demirel Üniversitesi}, year = {2019}, volume = {14}, pages = {71 - 76}, doi = {10.29233/sdufeffd.458082}, title = {Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry}, key = {cite}, author = {DERMİŞ, Saadet and KILIÇ, Sümeyye and ERTEKİN, Zehra Ceren and DİNÇ, Erdal} }
APA DERMİŞ, S , KILIÇ, S , ERTEKİN, Z , DİNÇ, E . (2019). Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi , 14 (1) , 71-76 . DOI: 10.29233/sdufeffd.458082
MLA DERMİŞ, S , KILIÇ, S , ERTEKİN, Z , DİNÇ, E . "Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 14 (2019 ): 71-76 <https://dergipark.org.tr/tr/pub/sdufeffd/issue/45380/458082>
Chicago DERMİŞ, S , KILIÇ, S , ERTEKİN, Z , DİNÇ, E . "Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 14 (2019 ): 71-76
RIS TY - JOUR T1 - Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry AU - Saadet DERMİŞ , Sümeyye KILIÇ , Zehra Ceren ERTEKİN , Erdal DİNÇ Y1 - 2019 PY - 2019 N1 - doi: 10.29233/sdufeffd.458082 DO - 10.29233/sdufeffd.458082 T2 - Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi JF - Journal JO - JOR SP - 71 EP - 76 VL - 14 IS - 1 SN - -1306-7575 M3 - doi: 10.29233/sdufeffd.458082 UR - https://doi.org/10.29233/sdufeffd.458082 Y2 - 2019 ER -
EndNote %0 Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry %A Saadet DERMİŞ , Sümeyye KILIÇ , Zehra Ceren ERTEKİN , Erdal DİNÇ %T Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry %D 2019 %J Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi %P -1306-7575 %V 14 %N 1 %R doi: 10.29233/sdufeffd.458082 %U 10.29233/sdufeffd.458082
ISNAD DERMİŞ, Saadet , KILIÇ, Sümeyye , ERTEKİN, Zehra Ceren , DİNÇ, Erdal . "Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 14 / 1 (Mayıs 2019): 71-76 . https://doi.org/10.29233/sdufeffd.458082
AMA DERMİŞ S , KILIÇ S , ERTEKİN Z , DİNÇ E . Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry. SDÜFEFFD. 2019; 14(1): 71-76.
Vancouver DERMİŞ S , KILIÇ S , ERTEKİN Z , DİNÇ E . Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi. 2019; 14(1): 76-71.