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Characterization of Myrrh Essential Oil wıth GC-MS and Investigation Antibacterıal Effects on Salmonella spp.

Yıl 2021, Cilt: 16 Sayı: 1, 319 - 327, 27.05.2021
https://doi.org/10.29233/sdufeffd.853138

Öz

Although Salmonella cause serious infections in animals, they are also of great importance with their zoonotic features. In this study, the antibacterial effects of myrrh (Commiphora myrrha) essential oil on poultry and human isolated Salmonella spp., S. typhimurium ATCC 14028, also Gram-positive (Staphylococcus aureus ATCC 25923, Methicillin Resistant Staphylococcus aureus ATCC 43300), Gram-negative (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922) were investigated agar well diffusion method and minimum inhibition concentrations (MICs) were determined micro dilution methods. In addition bioactive components analysed by gas chromatography-mass spectrophotometer GC / MS. According to the results of GC-MS, major component of myrrh essential oil is curzerene with 24.99 % rate. This was followed by beta elemene with 21.75%. A total of 106 components were detected, and the other components detected were found at between 0.01% and 1.88% rate. Antibacterial test result showed that the zone diameter range of essential oil on Salmonella spp. isolates have been determined between 7.75 mm and 9.75 mm and MIC values ranging from 12.4 µg / ml to 49.6 µg / ml. In reference strains, it was seen that the most effective result was on Gram-positive bacteria.

Teşekkür

As the authors of this study, we would like to thank Ankara University Faculty of Veterinary Medicine Microbiology Department for the poultry isolates used in the study and Diyarbakır University Faculty of Medicine Clinical Microbiology Department for clinical isolates.

Kaynakça

  • [1] A. Asımgil, “Şifalı bitkiler,” Timaş Yayınları, 176 (2), 16-307, 1997.
  • [2] S. Yaşar, “Determınation of fixed and essential oıl contents and soıl characteristic of some perennial medical plants that grow naturally in the campus of Cukurova Unıversıty,” M.S. thesis, Dept. Bio., Çukurova Univ., Adana, 2005.
  • [3] W. T. Langeveld., E. J. A. Veldhuizen, and S. A. Burt, “Synergy between essential oil components and antibiotics: a review,” Critical Reviews in Microbiology, 40 (1), 76-94, 2014.
  • [4] A. Başaran, “Natural aromatherapy: herbs & essences,” Turkiye Klinikleri J Med Sci., 29 (5), 86-94, 2009.
  • [5] S. A. Gadir, and I. M. Ahmed, “Commiphora myrrha and commiphora,” Africana essential oils. J. chem. Pharm., 6 (7), 151-156, 2014.
  • [6] S. Su, T. Wang, T. Chen, J. Duan, L. Yu, and T. Yuping, “Cytotoxicity activity of extracts and compounds from Commiphora myrrha resin against human gynecologic cancer cells,” J. Med. Plant Res., 5 (8), 1382-1389, 2011.
  • [7] M. F. Khan, H. Tang, J. T. Lyles, R. Pineau, Z. R. Mashwani, and C. L. Quave, “Antibacterial properties of medicinal plants from Pakistan against multidrug-resistant ESKAPE pathogens,” Front. Pharmacol., 9 (815), 1-17, 2018.
  • [8] M. Ö. Salar, H. Yardımcı, and K. S. Diker, “Antimicrobial effects of some industrial plants on Salmonella species,” Journal of Turkish Veterinary Medical Society., 86 (2), 9-18, 2015.
  • [9] E. E. Evans, “Zoonotic diseases of common pet birds: psittacine, passerine and columbiform species,” Vet Clin North Am Exot Anim Pract., 14, 457–476, 2011.
  • [10] G. Dinç, M. Doğanay, and M. İzgür, “Important bacterial infections transmitted to humans from pet animals,” Turk Hij Tecr Biyol Derg, 72 (2), 163-174, 2015.
  • [11] M. Akan, “Kanatlılarda salmonella infeksiyonları ve kontrolünde temel prensipler,” Mektup Ankara, 6, 3-4, 2008.
  • [12] A. Kutu, “Isolation, serotyping and investigation of antibiotic susceptıbilities of Salmonella species in poultry,” M.S. thesis, Dept. Mic., Adnan Menderes Uni., Aydın, 2017.
  • [13] M. K. Swamy, M. S. Akhtar, and U. R. Sinniah, “Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review,” Evidence-based complementary and alternative medicine., 3012462, 2016.
  • [14] F. Nazzaro, F. Fratianni, L. De Martino, R. Coppola, and V. De Feo, “Effect of essential oils on pathogenic bacteria,” Pharmaceuticals., 6 (12), 1451-74, 2013.
  • [15] J.S. Raut, and S. M. Karuppayil, “A status review on the medicinal properties of essential oils,” Ind Crops Prod., 62, 250-64, 2014.
  • [16] D. Trombetta, F. Castelli, M.G. Sarpietro, V. Venuti, M. Cristani, C. Daniele, A. Saija, G. Mazzanti, and G. Bisignano, “Mechanisms of antibacterial action of three monoterpenes,” Antimicrob Agents Chemother., 49(6), 2474-2478, 2005.
  • [17] I. A. Holder, and S. T. Boyce, “Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture,” Burns., 20 (5), 426-429, 1994.
  • [18] J. M. Andrews, “Determination of minimum inhibitory concentrations,” J. Antimicrob. Chemother., 48 (1), 5-16, 2001.
  • [19] C. Kürekci, and F. Sakin, “Essential oils:essential oils as antimicrobial: in-vitro and in-vivo studies,” Turkiye Klinikleri J Anim Nutr&Nut., 3 (1), 15-20, 2017.
  • [20] A. Lytovchenko, R. Beleggia, N. Schauer, T. Isaacson, J. E. Leuendorf, H. Hellmann, J. K. C. Rose, and A. R. Fernie, “Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues,” Plant Methods., 5 (4), 2009.
  • [21] S. R. Ahamad, A. R. Al-Ghadeer, R. Ali, W. Qamar and S. Aljarboa, “Analysis of inorganic and organic constituents of myrrh resin by GC–MS and ICP-MS: An emphasis on medicinal assets,” Saudi Pharmaceutical Journal, 25 (5), 788-794, 2017.
  • [22] J. Zhao, J. Zhang, B. Yang, G.P. Lv, and S. P. Li, “Free radical scavenging activity and characterization of sesquiterpenoids in four species of curcuma using a TLC bioautography assay and GC-MS analysis,” Molecules, 15, 7547-7557, 2010.
  • [23] Y. Chen, C. Zhou, Z. Ge, Y. Liu, W. Feng, S. LI, G. Chen, and T. WeiI, “Composition and potential anticancer activities of essential oils obtained from myrrh and frankincense,” Oncol. Lett., 6 (4), 1140–1146, 2013.
  • [24] C. F. Bagamboula, M. Uyttendaele, and J. Debevere. “Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri,” Food Microbiology, 21, 33–42, 2004.
  • [25] M. Hyldgaard, T. Mygind, and R. Meyer, “Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components,” Front. Microbiol., 3 (12), 1-24 2012.
  • [26] A. G. Mohamed, H. M. Abbas, J. M. Kassem, W. A. Gafour, and A. G. Attalah, “Impact of myrrh essential oil as a highly effective antimicrobial agent in processed cheese spreads,” Int. J. Dairy Sci., 11 (2), 41-51, 2016.
  • [27] N. Khalil, S. Fikry, and O. Salama, “Bactericidal activity of myrrh extracts and two dosage forms against standard bacterial strains and multidrug-resistant clinical isolates with GC/MS profling,” AMB Express, 10 (21), 1-10, 2020.

Mür Uçucu Yağının GC-MS ile Karakterizasyonu ve Salmonella spp. Üzerine Antibakteriyel Etkilerinin Araştırılması

Yıl 2021, Cilt: 16 Sayı: 1, 319 - 327, 27.05.2021
https://doi.org/10.29233/sdufeffd.853138

Öz

Salmonellalar hayvanlarda ciddi enfeksiyonlara neden olmalarının yanısıra zoonotik özelliklerinden dolayı da oldukça önemlidirler. Yapılan bu çalışma ile mür (Commiphora myrrha) uçucu yağının kanatlı ve insandan izole edilen Salmonella spp. suşları ile S. typhimurium ATCC 14028 referans suşu ve Gram-pozitif (Staphylococcus aureus ATCC 25923, Methicillin Resistant Staphylococcus aureus ATCC 43300), Gram-negatif (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922) bazı bakteriler üzerine antibakteriyel etkisi agar kuyucuk difüzyon yöntemi ile araştırılarak minimum inhibisyon konsantrasyonu (MİK) belirlenmiştir. Ayrıca biyoaktif bileşenleri gaz kromotografisi kütle spektrofotometre (GS/MS) ile araştırılmıştır. GS/MS sonuçlarına göre mür uçucu yağının sahip olduğu major bileşen %24,99 ile curzeren olup %21,75 ile beta elemenin ikinci major bileşen olduğu görülmüş ve oranları %0,01 ile %1,88 arasında değişen toplamda 106 bileşen tespit edilmiştir.

Kaynakça

  • [1] A. Asımgil, “Şifalı bitkiler,” Timaş Yayınları, 176 (2), 16-307, 1997.
  • [2] S. Yaşar, “Determınation of fixed and essential oıl contents and soıl characteristic of some perennial medical plants that grow naturally in the campus of Cukurova Unıversıty,” M.S. thesis, Dept. Bio., Çukurova Univ., Adana, 2005.
  • [3] W. T. Langeveld., E. J. A. Veldhuizen, and S. A. Burt, “Synergy between essential oil components and antibiotics: a review,” Critical Reviews in Microbiology, 40 (1), 76-94, 2014.
  • [4] A. Başaran, “Natural aromatherapy: herbs & essences,” Turkiye Klinikleri J Med Sci., 29 (5), 86-94, 2009.
  • [5] S. A. Gadir, and I. M. Ahmed, “Commiphora myrrha and commiphora,” Africana essential oils. J. chem. Pharm., 6 (7), 151-156, 2014.
  • [6] S. Su, T. Wang, T. Chen, J. Duan, L. Yu, and T. Yuping, “Cytotoxicity activity of extracts and compounds from Commiphora myrrha resin against human gynecologic cancer cells,” J. Med. Plant Res., 5 (8), 1382-1389, 2011.
  • [7] M. F. Khan, H. Tang, J. T. Lyles, R. Pineau, Z. R. Mashwani, and C. L. Quave, “Antibacterial properties of medicinal plants from Pakistan against multidrug-resistant ESKAPE pathogens,” Front. Pharmacol., 9 (815), 1-17, 2018.
  • [8] M. Ö. Salar, H. Yardımcı, and K. S. Diker, “Antimicrobial effects of some industrial plants on Salmonella species,” Journal of Turkish Veterinary Medical Society., 86 (2), 9-18, 2015.
  • [9] E. E. Evans, “Zoonotic diseases of common pet birds: psittacine, passerine and columbiform species,” Vet Clin North Am Exot Anim Pract., 14, 457–476, 2011.
  • [10] G. Dinç, M. Doğanay, and M. İzgür, “Important bacterial infections transmitted to humans from pet animals,” Turk Hij Tecr Biyol Derg, 72 (2), 163-174, 2015.
  • [11] M. Akan, “Kanatlılarda salmonella infeksiyonları ve kontrolünde temel prensipler,” Mektup Ankara, 6, 3-4, 2008.
  • [12] A. Kutu, “Isolation, serotyping and investigation of antibiotic susceptıbilities of Salmonella species in poultry,” M.S. thesis, Dept. Mic., Adnan Menderes Uni., Aydın, 2017.
  • [13] M. K. Swamy, M. S. Akhtar, and U. R. Sinniah, “Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review,” Evidence-based complementary and alternative medicine., 3012462, 2016.
  • [14] F. Nazzaro, F. Fratianni, L. De Martino, R. Coppola, and V. De Feo, “Effect of essential oils on pathogenic bacteria,” Pharmaceuticals., 6 (12), 1451-74, 2013.
  • [15] J.S. Raut, and S. M. Karuppayil, “A status review on the medicinal properties of essential oils,” Ind Crops Prod., 62, 250-64, 2014.
  • [16] D. Trombetta, F. Castelli, M.G. Sarpietro, V. Venuti, M. Cristani, C. Daniele, A. Saija, G. Mazzanti, and G. Bisignano, “Mechanisms of antibacterial action of three monoterpenes,” Antimicrob Agents Chemother., 49(6), 2474-2478, 2005.
  • [17] I. A. Holder, and S. T. Boyce, “Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture,” Burns., 20 (5), 426-429, 1994.
  • [18] J. M. Andrews, “Determination of minimum inhibitory concentrations,” J. Antimicrob. Chemother., 48 (1), 5-16, 2001.
  • [19] C. Kürekci, and F. Sakin, “Essential oils:essential oils as antimicrobial: in-vitro and in-vivo studies,” Turkiye Klinikleri J Anim Nutr&Nut., 3 (1), 15-20, 2017.
  • [20] A. Lytovchenko, R. Beleggia, N. Schauer, T. Isaacson, J. E. Leuendorf, H. Hellmann, J. K. C. Rose, and A. R. Fernie, “Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues,” Plant Methods., 5 (4), 2009.
  • [21] S. R. Ahamad, A. R. Al-Ghadeer, R. Ali, W. Qamar and S. Aljarboa, “Analysis of inorganic and organic constituents of myrrh resin by GC–MS and ICP-MS: An emphasis on medicinal assets,” Saudi Pharmaceutical Journal, 25 (5), 788-794, 2017.
  • [22] J. Zhao, J. Zhang, B. Yang, G.P. Lv, and S. P. Li, “Free radical scavenging activity and characterization of sesquiterpenoids in four species of curcuma using a TLC bioautography assay and GC-MS analysis,” Molecules, 15, 7547-7557, 2010.
  • [23] Y. Chen, C. Zhou, Z. Ge, Y. Liu, W. Feng, S. LI, G. Chen, and T. WeiI, “Composition and potential anticancer activities of essential oils obtained from myrrh and frankincense,” Oncol. Lett., 6 (4), 1140–1146, 2013.
  • [24] C. F. Bagamboula, M. Uyttendaele, and J. Debevere. “Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri,” Food Microbiology, 21, 33–42, 2004.
  • [25] M. Hyldgaard, T. Mygind, and R. Meyer, “Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components,” Front. Microbiol., 3 (12), 1-24 2012.
  • [26] A. G. Mohamed, H. M. Abbas, J. M. Kassem, W. A. Gafour, and A. G. Attalah, “Impact of myrrh essential oil as a highly effective antimicrobial agent in processed cheese spreads,” Int. J. Dairy Sci., 11 (2), 41-51, 2016.
  • [27] N. Khalil, S. Fikry, and O. Salama, “Bactericidal activity of myrrh extracts and two dosage forms against standard bacterial strains and multidrug-resistant clinical isolates with GC/MS profling,” AMB Express, 10 (21), 1-10, 2020.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Makaleler
Yazarlar

Evren Arın 0000-0002-6800-9226

Ebru Önem 0000-0002-7770-7958

Mehmet Ali Tabur 0000-0002-7016-6896

Yayımlanma Tarihi 27 Mayıs 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 16 Sayı: 1

Kaynak Göster

IEEE E. Arın, E. Önem, ve M. A. Tabur, “Characterization of Myrrh Essential Oil wıth GC-MS and Investigation Antibacterıal Effects on Salmonella spp”., Süleyman Demirel University Faculty of Arts and Science Journal of Science, c. 16, sy. 1, ss. 319–327, 2021, doi: 10.29233/sdufeffd.853138.