Araştırma Makalesi
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Fasulye Tohumlarında Fasulye Adi Mozayik Virüsü’nün Immunocapture RT-PCR ve DAS-ELISA Yöntemleriyle Teşhisi

Yıl 2022, , 1 - 7, 29.06.2022
https://doi.org/10.33484/sinopfbd.1006005

Öz

Bu çalışmada, 2016 yılında Isparta ve Antalya illerinde fasulye tohumlarında Fasulye adi mozayik virüsü (Bean common mosaic virus: BCMV)’nün belirlenmesi amacıyla yapılmıştır. Bu amaçla araştırma alanından 106 tohum örneği toplanmıştır. Tohum örnekleri biyolojik, serolojik ve moleküler çalışmalar için kullanılmıştır. DAS-ELISA testi sonucunda tohum örneklerinde BCMV’nün hastalık oranı %87.73 olarak belirlenirken, Immunocapture ters transkripsiyon polimeraz zincir reaksiyonu (IC-RT-PCR) yönteminde örneklerdeki hastalık oranı %90.56 olarak belirlenmiştir. Yürütülen çalışmalar sonucunda toplam 96 tohum örneğinde (%90.56) BCMV belirlenmiştir. DAS-ELISA yöntemi ile 93 örnek BCMV ile pozitif belirlenirken; IC-RT-PCR yöntemi ile 96 örnek BCMV ile enfekteli bulunmuştur. IC-RT-PCR yönteminde spesifik primerler ile kılıf protein geninin yaklaşık 850 bp’lik bir kısmı amplifiye edilmiş ve BCMV’ne özgü beklenen seviyede bant elde edilmiştir.

Kaynakça

  • Loebenstein, G., & Thottappilly, G. (2004). Virus and virus-like diseases of major crops in developing countries. Dordrecht, Kluwer Academic Publishing. 840.
  • TUIK (2016, October). Crop Production Statistics. http://tuikrapor.tuik.gov.tr/reports.
  • Nyvall, R.F. (1989). Field Crop Diseases Handbook. New York, Van Nostrand Reinhold, 791.
  • Matthews, R.E.F. (1991). Plant Virology. Academic Press Publishing.
  • Kumar, C. A., Khetarpal, R. K., Parakh, D.B., Singh, S., & Nath, R. (1994). In Check list on seed transmitted viruses, Leguminous hosts. National Bureau of Plant Genetic Resources, New Delhi.
  • Guzel, O., & Arli-Sökmen, M. (2003). Determination of some viruses infecting common bean (Phaseolus vulgaris L.) and their incidences in seed lots in Samsun Province. Journal of Turkish Phytopathology, 32(2), 99-106.
  • Ghorbani, S.G.M., Shahraeena, N., & Elahinia, S.A. (2010) Distribution and impact of virus associated diseases of common bean (Phaseolus vulgaris L.) in Northern Iran, Archives of Phytopathology and Plant Protection, 43(12), 1183–1189. https://doi.org/: 10.1080/03235400802366834
  • Omar, R. A, Mehiar, F. F., Zayed, E., & Deit, A. A. (1985). Biological studies on some seed-borne viruses and their effect on vegetative growth and yield component of the host plant, Acta Phytopathologica Academiae Scientiarum Hungaricae, 20(3), 80-85.
  • Drijfhout, E., & Morales, F. J. (2005). Compendium of bean diseases. In H. Schwartz, J.R. Steadman, R. Hall & R.L. Foster, (Ed), Bean mosaic virus, (pp. 63–64).
  • Fidan, U., & Yorganci, U. (1990). Investigation on the detection and seed transmission of the virus diseases occuring on the pulse crops in Aegean Region. Seed transmission of virus diseases by grower seeds and seeds of artificial infected pulse crops, Journal of Turkish Phytopathology, 19(1), 1-6.
  • Kutluk-Yilmaz, N.D., Gumus, M., & Erkan, S. (2002). Studies on determination of virus diseases in the seeds of bean from Tokat Province. Ege University, Journal of Agriculture Faculty, 38(3), 49-55.
  • Naghavi, A., Habibi M.K., & Firouzabadi, F. N. (2008). Detection and identification of some soybean viral mosaic viruses, using molecular techniques in Lorestan Province, Southwest of Iran, Asian Journal of Plant Science, 7(6), 557-562. https://doi.org/ 10.3923/ajps.2008.557.562
  • Bhadramurthy, V., & Bhat, A.I. (2009). Biological and molecular characterization of Bean common mosaic virus associated with vanilla in India. Indian Journal of Virology, 20(2), 70-77.
  • Udayashankar, A.C., Chandra-Nayaka, S., Niranjana, S.R., Mortensen, C.N., & Prakash, H.S. (2012). Immunocapture RT-PCR detection of Bean common mosaic virus and strain blackeye cowpe a mosaic in common bean and black gram in India. Archives of Phytopathology and Plant Protection, 45(13), 1509–1518. https://doi.org/10.1080/03235408.2012.679123
  • Hosseini, A., & Hosseini, S. (2014). Occurrence and distribution of Bean common mosaic virus and Bean yellow mosaic virus from common bean fields of Kerman province, Iran, Indian Journal of Fundamental Applied Life Sciences, 4(2), 528 -535.
  • Johary, T., Dizadji, A., & Naderpour, M. (2016). Biological and molecular characteristics of Bean common mosaic virus isolates circulating in common bean in Iran. Journal of Plant Pathology, 98(2), 301-310. https://dx.doi.org/10.4454/JPP.V98I2.027
  • [Hao, N.B., Albrechtsen, S.E., & Nicolaisen, M. (2003). Detection and identification of the blackeye cowpea mosaic strain of Bean common mosaic virus in seeds of Vigna unguiculata spp. from North Vietnam, Australas Plant Pathology, 32, 505–509. https://doi.org/10.1071/AP03052
  • Vinayarani, G., Madhusudhan, K.N., Deepak, S.A., Niranjana, S.R., & Prakash, H.S. (2011). Detection of mixed infection of tobamoviruses in tomato and bell pepper by using RT-PCR and duplex RT-PCR. International journal of Plant Pathology, 2, 89-95. https://doi.org//10.3923/ijpp.2011.89.95
  • Abtahi, F.S., Hbibi, M.K., & Motlagh, M.K. (2009). Some biological and molecular characterization of Bean common mosaic necrosis virus isolated from soybean in Tehran Province, Iran. World Academy Science, Engineering and Technology, 49, 126-129.
  • Petrovic D, Ignjatov, M., Nikolic, Z., Vujakovic, M., Vasic, M., Milosevic, M., & Taski-Ajdukovic, K. (2010). Occurrence and Distribution of viruses infecting the bean in Serbia. Archives of Biological Science, 62(3), 595-601. https://doi.org/doi. 10.2298/ABS1003595P

Detection of Bean Common Mosaic Virus in Bean Seeds by Immunocapture RT-PCR and DAS-ELISA Methods

Yıl 2022, , 1 - 7, 29.06.2022
https://doi.org/10.33484/sinopfbd.1006005

Öz

This study was carried out in bean seeds in Isparta and Antalya provinces to determine Bean common mosaic virus (BCMV) in 2016. For this purpose; 106 seed samples were collected from the research area. Seed samples were used for biological, serological, and molecular methods. The virus incidence, as a percentage of seeds samples for BCMV, was found 87.73% by DAS-ELISA and 90.56% by Immunocapture Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-PCR) methods. As a result of the study, BCMV was detected in a total of 96 (90.56%) seed samples. 93 samples were detected positive with the DAS-ELISA method, while 96 samples were found to be infected with BCMV using the IC-RT-PCR method. In the IC-RT-PCR method, approximately 850 bp of the coat protein gene was amplified with specific primers and BCMV specific bands were obtained at the expected levels.

Kaynakça

  • Loebenstein, G., & Thottappilly, G. (2004). Virus and virus-like diseases of major crops in developing countries. Dordrecht, Kluwer Academic Publishing. 840.
  • TUIK (2016, October). Crop Production Statistics. http://tuikrapor.tuik.gov.tr/reports.
  • Nyvall, R.F. (1989). Field Crop Diseases Handbook. New York, Van Nostrand Reinhold, 791.
  • Matthews, R.E.F. (1991). Plant Virology. Academic Press Publishing.
  • Kumar, C. A., Khetarpal, R. K., Parakh, D.B., Singh, S., & Nath, R. (1994). In Check list on seed transmitted viruses, Leguminous hosts. National Bureau of Plant Genetic Resources, New Delhi.
  • Guzel, O., & Arli-Sökmen, M. (2003). Determination of some viruses infecting common bean (Phaseolus vulgaris L.) and their incidences in seed lots in Samsun Province. Journal of Turkish Phytopathology, 32(2), 99-106.
  • Ghorbani, S.G.M., Shahraeena, N., & Elahinia, S.A. (2010) Distribution and impact of virus associated diseases of common bean (Phaseolus vulgaris L.) in Northern Iran, Archives of Phytopathology and Plant Protection, 43(12), 1183–1189. https://doi.org/: 10.1080/03235400802366834
  • Omar, R. A, Mehiar, F. F., Zayed, E., & Deit, A. A. (1985). Biological studies on some seed-borne viruses and their effect on vegetative growth and yield component of the host plant, Acta Phytopathologica Academiae Scientiarum Hungaricae, 20(3), 80-85.
  • Drijfhout, E., & Morales, F. J. (2005). Compendium of bean diseases. In H. Schwartz, J.R. Steadman, R. Hall & R.L. Foster, (Ed), Bean mosaic virus, (pp. 63–64).
  • Fidan, U., & Yorganci, U. (1990). Investigation on the detection and seed transmission of the virus diseases occuring on the pulse crops in Aegean Region. Seed transmission of virus diseases by grower seeds and seeds of artificial infected pulse crops, Journal of Turkish Phytopathology, 19(1), 1-6.
  • Kutluk-Yilmaz, N.D., Gumus, M., & Erkan, S. (2002). Studies on determination of virus diseases in the seeds of bean from Tokat Province. Ege University, Journal of Agriculture Faculty, 38(3), 49-55.
  • Naghavi, A., Habibi M.K., & Firouzabadi, F. N. (2008). Detection and identification of some soybean viral mosaic viruses, using molecular techniques in Lorestan Province, Southwest of Iran, Asian Journal of Plant Science, 7(6), 557-562. https://doi.org/ 10.3923/ajps.2008.557.562
  • Bhadramurthy, V., & Bhat, A.I. (2009). Biological and molecular characterization of Bean common mosaic virus associated with vanilla in India. Indian Journal of Virology, 20(2), 70-77.
  • Udayashankar, A.C., Chandra-Nayaka, S., Niranjana, S.R., Mortensen, C.N., & Prakash, H.S. (2012). Immunocapture RT-PCR detection of Bean common mosaic virus and strain blackeye cowpe a mosaic in common bean and black gram in India. Archives of Phytopathology and Plant Protection, 45(13), 1509–1518. https://doi.org/10.1080/03235408.2012.679123
  • Hosseini, A., & Hosseini, S. (2014). Occurrence and distribution of Bean common mosaic virus and Bean yellow mosaic virus from common bean fields of Kerman province, Iran, Indian Journal of Fundamental Applied Life Sciences, 4(2), 528 -535.
  • Johary, T., Dizadji, A., & Naderpour, M. (2016). Biological and molecular characteristics of Bean common mosaic virus isolates circulating in common bean in Iran. Journal of Plant Pathology, 98(2), 301-310. https://dx.doi.org/10.4454/JPP.V98I2.027
  • [Hao, N.B., Albrechtsen, S.E., & Nicolaisen, M. (2003). Detection and identification of the blackeye cowpea mosaic strain of Bean common mosaic virus in seeds of Vigna unguiculata spp. from North Vietnam, Australas Plant Pathology, 32, 505–509. https://doi.org/10.1071/AP03052
  • Vinayarani, G., Madhusudhan, K.N., Deepak, S.A., Niranjana, S.R., & Prakash, H.S. (2011). Detection of mixed infection of tobamoviruses in tomato and bell pepper by using RT-PCR and duplex RT-PCR. International journal of Plant Pathology, 2, 89-95. https://doi.org//10.3923/ijpp.2011.89.95
  • Abtahi, F.S., Hbibi, M.K., & Motlagh, M.K. (2009). Some biological and molecular characterization of Bean common mosaic necrosis virus isolated from soybean in Tehran Province, Iran. World Academy Science, Engineering and Technology, 49, 126-129.
  • Petrovic D, Ignjatov, M., Nikolic, Z., Vujakovic, M., Vasic, M., Milosevic, M., & Taski-Ajdukovic, K. (2010). Occurrence and Distribution of viruses infecting the bean in Serbia. Archives of Biological Science, 62(3), 595-601. https://doi.org/doi. 10.2298/ABS1003595P
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Handan Çulal Kılıç 0000-0003-4020-9442

Ahmet Çat 0000-0002-5638-0319

Yayımlanma Tarihi 29 Haziran 2022
Gönderilme Tarihi 7 Ekim 2021
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Çulal Kılıç, H., & Çat, A. (2022). Detection of Bean Common Mosaic Virus in Bean Seeds by Immunocapture RT-PCR and DAS-ELISA Methods. Sinop Üniversitesi Fen Bilimleri Dergisi, 7(1), 1-7. https://doi.org/10.33484/sinopfbd.1006005


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