Bazı Doğal Biyomateryallerin Antioksidan/Oksidan Potansiyelinin Araştırılması

Öz

Son zamanlarda yapılan çalışmaların çoğu, organizmada oksidatif stresin oluşturduğu zararlara çare veya önleyici olarak görev yapan bitki bazlı antioksidatif materyaller ve biyomateryaller üzerine yoğunlaşmıştır. Antioksidanların potansiyel düzeyini değerlendirmek için çeşitli yollar sağlayan farklı yöntemler vardır. Antioksidanlar, biyolojik hücrelerde bulunan ve canlı organizmalar üzerinde olumsuz etkiye sahip olan serbest radikalleri nötralize etmektedir. Bu çalışmanın amacı, kısa süreli iskemi/reperfüzyon (I/R) ile ortaya çıkan hasarlı dokuyu tedavi eden biyomateryaller olarak bilinen naringin, silymarin ve ellagik asitlerin potansiyel koruyucu etkilerinin $in$ $vivo$ olarak incelenmesidir. Bu çalışmada, 3 haftalık, klinik olarak sağlıklı, 250-300 g ağırlığında Wistar albino rat (n: 35) kullanıldı. Sıçanlardan alınan kan örneklerinde Plazma Paraoksonaz (PON), toplam sialik asit (TSA), toplam antioksidan kapasite (TAK) ve toplam oksidan kapasite (TOK), oksidatif stres indeksi (OSI) ve Nitrik Oksit (NO) analiz edildi. Sonuçlar, biyomateryal kullanımından sonra TSA, TOK ve NO düzeylerinin azaldığını, TAK ve PON düzeylerinin ise arttığını ortaya koymaktadır ($P$<0.05). Bu çalışma aynı zamanda silymarin, naringin ve ellagik asitlerin serbest radikal temizleyici, antiinflamatuar, radyoprotektif, antiülserojenik, analjezik ve antikarsinojenik özelliklerinin varlığını da doğruladı. Elde edilen $in$ $vivo$ sonuçlar göz önüne alındığında, $in$ $vivo$ ve $in$ $vitro$ uygulamalarda oksidatif stresin etkilerini ve düzeyini azaltmak için umut verici bir aday olarak değerlendirilebilecekleri sonucuna varılmıştır.

Anahtar Kelimeler

• Serbest radikal
• iskemi/reperfüzyon
• nitrik oksit
• toplam antioksidan/oksidan kapasite

Investigation of Antioxidant/Oxidant Potential of Some Natural Biomaterials

Öz

Recent studies have focused on plant-based antioxidative materials and biomaterials that serve as remedies or preventive agents against oxidative stress-induced damage in organisms. Various methods exist to evaluate the antioxidant potential of these compounds. Antioxidants neutralize free radicals in biological cells, mitigating their harmful effects on living organisms. This study aimed to investigate the $in$ $vivo$ protective effects of naringin, silymarin and ellagic acid which are known to contribute to tissue repair following short-term ischemia/reperfusion (I/R) injury. Three-week-old, clinically healthy, Wistar albino rats (n: 35), weighing 250-300 g, were used in this study. Blood samples were analyzed for plasma paraoxonase (PON), total sialic acid (TSA), total antioxidant capacity (TAC) and total oxidant capacity (TOC), oxidative stress index (OSI) and nitric oxide (NO) levels. Results demonstrated that TSA, TOC and NO levels decreased while TAC and PON levels increased following biomaterial administration ($P$<0.05). This study confirmed that silymarin, naringin and ellagic acid exhibit free radical scavenging, anti-inflammatory, radioprotective, antiulcerogenic, and analgesic properties. Given the obtained $in$ $vivo$ results, we conclude that, they might be considered as promising candidates to reduce the effects and the levels of oxidative stress during $in$ $vivo$ and $in$ $vitro$ applications

Anahtar Kelimeler

• Free radical
• ischemia/reperfusion
• nitric oxide
• total antioxidant/oxidant capacity

Kaynakça

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