Hypericum Perforatum (Sarı Kantaron) Çiçek Özütünden Ag/Ag2O Nanokompozitlerin Yeşil Sentez Yaklaşımı ile Üretimi ve Karakterizasyonu

Öz

Gümüş nanopartiküllerin yeşil sentezi, nanoteknoloji alanındaki en önemli konulardan biri olup modern malzeme biliminin çeşitli yönlerini derinlemesine etkilemektedir. Bu çalışmada, Hypericum perforatum çiçeklerinin sulu ekstresi, gümüş ve gümüş oksit (Ag/Ag₂O) nanokompozitlerinin yeşil sentezi için kullanılmıştır. Çiçek ekstresinin UV–Vis spektrumunda, ortam sıcaklığında 450 nm’de güçlü bir absorpsiyon pikine rastlanmıştır. Tauc grafiği kullanılarak belirlenen tahmini enerji bant aralığı (Eg) yaklaşık 3.66 eV olarak bulunmuştur. XRD analizleri, tipik yüz merkezli kübik (fcc) yapıya sahip saf biyojenik Ag/Ag₂O nanokompozitlerinin oluşumunu doğrulamış ve (111) düzlemine göre kristalit boyutu yaklaşık 6.6 nm olarak hesaplanmıştır. FTIR analizi, AgNO₃’ün kısmi biyoreduksiyonuna katkıda bulunan biyoaktif fonksiyonel grupların varlığını ortaya koymuştur. Ayrıca, yaklaşık 653–816 cm⁻¹ aralığında gözlenen absorpsiyon bantları, Ag/Ag₂O gerilme titreşimlerine atfedilmiş olup nanokompozitlerin yeşil sentezini doğrulamaktadır. Her ne kadar pek çok çalışma H. perforatum kullanılarak gümüş nanoparçacıkların yeşil sentezini rapor etmiş olsa da, bu bitki ekstresi ile Ag/Ag₂O nanokompozitlerinin doğrudan sentezine dair çalışmalar nadirdir; bu durum mevcut çalışmanın yenilik ve önemini ortaya koymaktadır. Gelecekteki çalışmalar, bu biyolojik olarak sentezlenen nanopartiküllerin ileri malzemeler ve kaplama sistemlerine entegrasyonunu araştırarak fonksiyonel özelliklerinin daha da geliştirilmesini hedefleyebilir.

Anahtar Kelimeler

• Ag/Ag2O
• yeşil sentez
• H. perforatum
• nanokompozitler

Green Synthesis and Characterization of Ag/Ag₂O Nanocomposites from the Flower Extract of Hypericum perforatum

Öz

The green synthesis of silver nanoparticles is one of the most significant topics in nanotechnology, profoundly impacting various aspects of modern materials science. In this study, an aqueous flower extract of Hypericum perforatum was utilized for the green synthesis of silver and silver oxide (Ag/Ag₂O) nanocomposites. UV–Vis spectra of the flower extract exhibited a strong absorption peak at 450 nm at ambient temperature. The estimated energy band gap (E₉) was approximately 3.66 eV, as determined using Tauc’s plot equation. XRD analysis confirmed the formation of pure biogenic Ag/Ag₂O nanocomposites with a typical face-centered cubic (fcc) structure, and the crystallite size along the (111) plane was estimated to be around 6.6 nm. FTIR analysis revealed the presence of bioactive functional groups that contributed to the partial bioreduction of AgNO₃ into Ag/Ag₂O nanocomposites. Additionally, absorption bands observed at approximately 653–816 cm⁻¹ were attributed to Ag/Ag₂O stretching vibrations, confirming the green synthesis of the nanocomposites. Although numerous studies report the green synthesis of silver nanoparticles using H. perforatum (St. John’s wort), direct reports on the formation of Ag/Ag₂O nanocomposites via this plant extract are scarce, highlighting the novelty and significance of the present work. Future studies could explore the incorporation of these biologically synthesized nanoparticles into advanced materials and coating systems to further enhance their functional properties.

Anahtar Kelimeler

• Ag/Ag₂O
• green synthesis
• H. perforatum
• nanocomposites

Destekleyen Kurum

This study was not supported by any institution and/or organization.

Etik Beyan

The study does not require ethics committee approval or any special permission.

Teşekkür

Author thanks CUMERLAB and Asst. Prof. Deniz Kadir TAKCI for characterization analysis.

Kaynakça

1. Faisal, S., Khan, M. A., Jan, H., Shah, S. A., Shah, S., Rizwan, M., & Akbar, M. T. (2021). Edible mushroom (Flammulina velutipes) as biosource for silver nanoparticles: From synthesis to diverse biomedical and environmental applications. Nanotechnology, 32(6), 065101. https://doi.org/10.1088/1361-6528/abc2eb
2. Yaqoob, A. A., Ahmad, H., Parveen, T., Ahmad, A., Oves, M., Ismail, I. M. I., Qari, H. A., Umar, K., & Ibrahim, M. N. M. (2020). Recent advances in metal decorated nanomaterials and their various biological applications: A review. Frontiers in Chemistry, 8, 341. https://doi.org/10.3389/fchem.2020.00341
3. Takcı, D. K., Ozdenefe, M. S., & Genc, S. (2023). Green synthesis of silver nanoparticles with an antibacterial activity using Salvia officinalis aqueous extract. Journal of Crystal Growth, 614, 127239. https://doi.org/10.1016/j.jcrysgro.2023.127239
4. Akhil, R., Jyoti, R., & Mira, D. (2019). Green synthesis of silver nanoparticles from Tectona grandis seeds extract: Characterization and mechanism of antimicrobial action on different microorganisms. Journal of Analytical Science and Technology, 10, 5. https://doi.org/10.1016/j.jallcom.2022.168130
5. Margarita, I. S., Victoria, I. V., Alexander, P., & Natalya, P. M. (2020). Green synthesis of silver nanoparticles in the presence of polysaccharide: Optimization and characterization. Journal of Nanomaterials, 2020, 3051308. https://doi.org/10.1155/2020/3051308
6. Sudip, S., Biraj, S., Kinkar, B., Tushar, K. J., Debjoy, B., Paulami, D., Rittick, M., Anoop, K., Apurba, K. D., Abdul, S., Soumen, S., Ahmet, K., Ismail, O., Octavio, L. F., Amitava, M., Sukhendu, M., Amit, K., & Ikbal, A. I. (2020). Bio-molecule functionalized rapid one-pot green synthesis of silver nanoparticles and their efficacy toward multidrug resistant (MDR) gut bacteria of silkworms (Bombyx mori). RSC Advances, 10, 22742–22757. https://doi.org/10.1039/d0ra03451g
7. Ahmed, S., Ahmad, M., Swami, B. L., & Ikram, S. (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7(1), 17–28. https://doi.org/10.1016/j.jare.2015.02.007
8. Huq, M. A., Ashrafudoulla, M., Rahman, M. M., Balusamy, S. R., & Akter, S. (2022). Green synthesis and potential antibacterial applications of bioactive silver nanoparticles: A review. Polymers, 14(4), 742. https://doi.org/10.3390/polym14040742

9. Ahsan, A., Farooq, M. A., Ahsan Bajwa, A., & Parveen, A. (2020). Green synthesis of silver nanoparticles using Parthenium hysterophorus: Optimization, characterization and in vitro therapeutic evaluation. Molecules, 25(15), 3324. https://doi.org/10.3390/molecules25153324
10. Takcı, H. A. M., Takcı, D. K., Icigen, S., Ozkan, H., Duman, S. E., & Huner, T. (2025). Manufacturing of Ag/Ag₂O nanocomposites for anti-quorum sensing: A safe and green approach. Journal of Crystal Growth, 652, 128041. https://doi.org/10.1016/j.jcrysgro.2025.128041
11. Celebioglu, A., Topuz, F., Yildiz, Z. I., & Uyar, T. (2019). One-step green synthesis of antibacterial silver nanoparticles embedded in electrospun cyclodextrin nanofibers. Carbohydrate Polymers, 207, 471–479. https://doi.org/10.1016/j.carbpol.2018.12.011
12. Takcı, D. K., Genc, S., & Takcı, H. A. M. (2023). Cinnamon-based rapid biosynthesis of silver nanoparticles: Its characterization and antibacterial properties. Journal of Crystal Growth, 623, 127416. https://doi.org/10.1016/j.jcrysgro.2023.127416
13. Göktürk Baydar, N., Babalik, Z., Demirci, T., Cessur, A. (2025). Synthesis methods impact silver nanoparticle properties and phenolic compound production in grapevine cell cultures. Scientific Reports, 15, 7667. https://doi.org/10.1038/s41598-025-85545-7
14. Sharma, S., Sharma, N., & Kaushal, N. (2022). Comparative account of biogenic synthesis of silver nanoparticles using probiotics and their antimicrobial activity against challenging pathogens. BioNanoScience, 12, 833–840. https://doi.org/10.1007/s12668-022-01004-x
15. Laouini, S. E., Bouafia, A., Soldatov, A. V., Algarni, H., Tedjani, M. L., Ali, G. A. M., & Barhoum, A. (2021). Green synthesis of Ag/Ag₂O nanoparticles using aqueous leaves extracts of Phoenix dactylifera L. and their azo dye photodegradation. Membranes, 11(7), 468. https://doi.org/10.3390/membranes11070468
16. Zaman, Y., Ishaque, M. Z., Ajmal, S., Shahzad, M., Siddique, A. B., Hameed, M. U., Kanwal, H., Ramalingham, R. J., Selvaraj, M., & Yasin, G. (2023). Tamed synthesis of AgNPs for photodegradation and antibacterial activity: Effect of size and morphology. Inorganic Chemistry Communications, 150, 110523. https://doi.org/10.1016/j.inoche.2023.110523
17. Chen, Y., Wei, Y., Chang, P., & Ye, L. (2011). Morphology-controlled synthesis of monodisperse silver spheres via a solvothermal method. Journal of Alloys and Compounds, 509(17), 5381–5387. https://doi.org/10.1016/j.jallcom.2011.02.001
18. Niggli, P. (1926). Skrifter utgitt av det Norske Videnskaps-Akademi i Oslo. I. Matematisk-Naturvidenskapelig Klasse, 1, 1–117.
19. Shahriari, E., Raeisi, M., & Aliakbari, K. (2024). Green synthesis of nanocomposite of Ag/Ag₂O using coelomic liquid: Optical absorption analysis. Nanochemistry Research, 9(4), 323–333. https://doi.org/10.22036/NCR.2024.04.08
20. Azzi, M., Medila, I., Toumi, I., et al. (2024). Plant extract-mediated synthesis of Ag/Ag₂O nanoparticles using Olea europaea leaf extract: Assessing antioxidant, antibacterial, and toxicological properties. Biomass Conversion and Biorefinery, 14, 31309–31322. https://doi.org/10.1007/s13399-023-05093-w
21. Kayed, K., Issa, M., & Al-Ourabi, H. (2024). The FTIR spectra of Ag/Ag₂O composites doped with silver nanoparticles. Journal of Experimental Nanoscience, 19(1). https://doi.org/10.1080/17458080.2024.2336227
22. Yousaf, A., Waseem, M., Javed, A., Baig, S., Ismail, B., Baig, A., Shahzadi, I., Nawazish, S., & Zaman, I. (2022). Augmented anticancer effect and antibacterial activity of silver nanoparticles synthesized by using Taxus wallichiana leaf extract. PeerJ, 10, e14391. https://doi.org/10.7717/peerj.14391
23. Masho, T. J., Ayane, M. M., Endale, G. N., Adula, S. H., Negasa, Y. D., Likasa, B. W., & Jida, S. M. (2025). Biosynthesis and characterization of Ag₂O nanoparticles for enhancement of antioxidant and photodegradation activities. Results in Chemistry, 13, 101919. https://doi.org/10.1016/j.rechem.2024.101919
24. Dagher, W., Alassod, A., Al Hinnawi, M. F., Alghoraibi, I., Taher, A., & Alnhlaoui, M. (2025). Characterization and antibacterial effect of green-synthesised silver nanoparticles using different extraction methods from Ziziphus spina-christi (Sidr) leaf extract collected from Syria. RSC Advances, 15(42), 35642-35659. https://doi.org/10.1039/D5RA05214A
25. Boudagha, S. E. I., Sobhi, C., Bendif, H., Uyanikgil, E. O. Ç., Abdennouri, A., Okeer, M., & Garzoli, S. (2025). Green synthesis of silver nanoparticles using cynoglossum creticum leaf extract: eco-friendly approach for antibacterial, antioxidant, and sensing applications. Applied Biochemistry and Biotechnology, 197, 7981–8007 https://doi.org/10.1007/s12010-025-05390-2