Endemik Teucrium leucophyllum (Lamiaceae) tohumlarında in vitro çimlendirme çalışmaları

Yıl 2016, Cilt: 1 Sayı: 1, 53 - 61, 09.08.2016

Muhip Hilooğlu , Ersin Yücel , Ali Kandemir , Emel Sözen

Öz

Teucrium leucophyllum Montbret & Aucher ex
Bentham (Lamiaceae), Erzincan yöresinde
(Türkiye) dar yayılış alanına sahip nadir endemik bitki türlerindendir. Bu
çalışmada Teucrium leucophyllum
türünde farklı NaCl, HCl, KNO₃, GA₃ konsantrasyonları,
sıcak-soğuk stratifikasyon ve mekanik skarifikasyon uygulamalarının çimlenme
başarısına etkilerinin incelenmesi amaçlanmıştır. Tohumlar 100 ve 200 μM konsantrasyonlarda NaCl, HCl, KNO₃, GA₃ solusyonları
kullanılarak ya da sıcak-soğuk ön işlem ve mekanik uygulamalarının ardından
23/18°C’de 8 saat aydınlık/16 saat karanlık ortamda çimlendirilmiştir. Kontrol
grubuyla karşılaştırıldığında (% 13.5), çimlenmenin
100-200µM GA₃ (% 52.5 ve %
51.5), için 100-200 µM KNO₃ (% 29 ve %28), zımpara ve 4°C’de ön üşütme uygulamalarıyla %25 oranında
arttığı (DMRT, P0.05) gözlenmiştir.
Çimlendirme hızı katsayısının ise en yüksek giberellik asit uygulamalarında
(11.4 ve 13.09) olduğu bulunmuştur. Bu çalışma sonucunda, elde edilen veriler
türün ex-situ korunması amacıyla
kullanılabilecektir.

Anahtar Kelimeler

Teucrium leucophyllum, Lamiaceae, endemik, çimlenme

Kaynakça

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