Renal İskemi-Reperfüzyon Hasarında Antioksidan Olarak Klorojenik Asit (CGA) Kullanımının İncelenmesi: Deneysel Çalışma

Yıl 2018, , 410 - 415, 29.06.2018

Ayhan Tanyeli , Derya Güzel

https://doi.org/10.31832/smj.420594

Öz

ÖZET

Amaç: Bu çalışma, sıçanlarda deneysel olarak
oluşturulan böbrek iskemi-reperfüzyon hasarında güçlü antioksidan olan Klorojenik asitin antiinflamatuar ve
antioksidan etkilerini araştırmayı hedeflemektedir.

Gereç ve Yöntemler: Otuz altı adet erişkin,
Wistar albino cinsi dişi sıçan, rastgele dört gruba ayrıldı. Sham grubundaki
sıçanlar (Grup I), sırt bölgesinden açılarak sağ nefrektomi yapıldı.
İskemi-reperfüzyon grubunda (Grup II), sıçanların sırt bölgesi açılıp sağ nefrektomi
yapıldıktan sonra sol renal arter klemplendi. Grup III ‘te 5 mg/kg Klorojenik
asit iskemiden 10 dk önce ve reperfüzyondan 10 dk önce olmak üzere iki doz
şeklinde intraperitoneal olarak uygulandı. Sol böbreğe 1 saatlik iskemiden
sonra 24 saatlik reperfüzyon uygulandı. Grup IV’te Grup III’ten farklı olarak
10 mg/kg Klorojenik asit  uygulandı.
Böbrek dokusunda total antioksidan düzeyi (TAS), total oksidan düzeyi (TOS),
süperoksit dismutaz (SOD), malondialdehit (MDA) ve myeloperoksidaz (MPO)
seviyeleri spektrofotometrik yöntemlerle ölçüldü. Oksidatif stres indeksi (OSI)
hesaplandı.

Bulgular: Grup II’de Grup I’e kıyasla TOS, MDA ve MPO
düzeylerinin arttığı; TAS ve SOD düzeylerinin
ise azaldığı görüldü (her bir parametre için p<0.001). Klorojenik
asit uygulanan gruplarda böbrek TOS (p<0.001), MDA (p<0.001)
ve MPO (p<0.001) düzeylerinde azalma;
TAS (p<0.05) ve SOD (p<0.001)
değerlerinde ise artış tespit edildi.

Sonuç: Böbrek
İ/R hasarında oksidatif stresin önemli rol oynadığı ve bu hasara karşı
Klorojenik asit   uygulamasının oksidatif
hasarı belirgin olarak azalttığı görüldü.

Anahtar Kelimeler

Klorojenik asit, renal iskemi reperfüzyon, oksidatif hasar

Kaynakça

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