Investigation of effect of probiotic use on organism behaviours in Drosophila melanogaster

Year 2022, , 1 - 13, 03.01.2023

Mehmet Fidan , Arif Ayar

https://doi.org/10.55895/sshs.1201900

Abstract

Microbiota; Microbiome refers to the genes carried by microorganisms living commensally with humans. Recent studies have shown that the gut microbiota plays an important role in shaping the health and fitness of animals, including humans. Probiotics, known as live microorganisms that, when taken in sufficient quantities, are beneficial to the health of the organism they inhabit, have been used traditionally by people for centuries in different ways.
In this study, it was aimed to investigate the effect of modified gut microbiota of Drosophila melanogaster individuals fed on probiotic supplemented media and standard media on mate selection, which is one of the behavioral characteristics. For this purpose, probiotic food containing 11 different bacterial species was added to the medium of D. melanogaster for 6 generations to reshape the fly microbiota. Flies grown on standard Drosophila medium and flies fed probiotic food were placed on the cell plates as 1 male and 1 female mixed. The mate choices of individuals carrying different markers were tracked and recorded. Obtained results were evaluated statistically.
As a result, it was observed that nutrition, and therefore microbiota, was effective in the selection of mates of flies mixed in cell plates. In particular, it was determined that individuals fed with probiotics chose the opposite sex fed with probiotics in the selection of mates for mating. It has been concluded that the effects of microbiota, the importance of which has been better understood in recent years, on living things should be clarified by different studies.

Keywords

Probiotic, Mate Selection, Microbiota, Drosophila melonagaster

References

• Alp, G., Aslim, B. (2009). The importance of bifidobacteria as probiotics in the human intestinal tract. Anadolu University Journal of Science and Technology 10(2): 343-354.
• Ayar, A. , Altun Çolak, D. , Uysal, H. & Fidan, M. (2021). Lifespan in Drosophila melanogaster Feeding with White Tea. Erzincan University Journal of Science and Technology , 14 (2) , 582- 594.
• Borre, Y.E, O’keeffe G.W., Clarke, G., Stanton, C., Dinan, T.G., Cryan, J.F. (2014). Microbiota and neurodevelopmental windows: implications for brain disorders. Trends Mol Med, 20:509-518.
• Deusch, S., Tilocca, B., Camarinha-Silva, A., Seifert, J. (2015). News in livestock research - use of Omics-technologies to study the microbiota in the gastrointestinal tract of farm animals. Computation Struct Biotechnol J, 13, 55–63.
• Dodd, D. M., Powell, J. R. (1985). Founder‐flush speciation: an update of experimental results with Drosophila. Evolution, 39(6), 1388-1392.
• Douglas, A. E. (2018). The Drosophila model for microbiome research. Lab Animal, 47(6), 157. Fidan, M., Ayar, A., & Konar, V. (2020). Effects of Perga (Bee bread) on Metamorphosis, Mortality, and Fecundity in Drosophila melanogaster. Sabuncuoglu Serefeddin Health Sciences, 2(2), 1- 15.
• Fidan, M., & Ayar, A. (2021a). Genetically Modified Organisms and Effects on Human Health. Research & Reviews in Health Sciences-I, 901.
• Fidan, M., and Ayar, A. (2021b). Comparison of The Effects of Different Forms of Green Tea on Fecundity in Drosophila Melanogaster. Sabuncuoglu Serefeddin Health Sciences, 3(2), 26-40.
• Fidan, M., & Ayar, A. (2021c). Modulatory effects of white tea (Camellia sinensis L.) on genotoxicity in streptozotocin and cyclophosphamide-treated Drosophila melanogaster. Progress in Nutrition, 23(3).
• Fidan, M., & Ayar, A. (2022). The Effect of Walnut Milk on Vital Parameters and Life Span. Health & Science 2022-Ii, 151.
• Fond, G., Boukouaci, W., Chevalier, G., Regnault, A., Eberl, G., Hamdani, N. (2014). The ‘‘psychomicrobiotic’’: Targeting microbiota in major psychiatric disorders: A systematic review. Pathol Biol (Paris), Epub ahead of print.
• Hanning, I, Diaz-Sanchez, S. (2015). The functionality of the gastrointestinal microbiome in nonhuman animals. Microbiome, 3, 51.
• Julian, R. (2014). Marchesi, The Human Microbiota and Microbiome, 124-35.
• Levy, M., Blacher, E., Elinav, E. (2017). Microbiome, metabolites and host immunity. Curr Opin Microbiol, 35, 8-15.
• Oelschlaeger, T.A. Definition of “good bacteria” origin, effects and effective components. Online: http://www.dgmim.de/fileadmin/CONTENT/OElschla eger_good_bacteria_vortrag.pdf, 2011, Erişim Tarihi: 04.03.2012
• Rosenberg, E., Zilber-Rosenberg I. (2011). Symbiosis and development: the hologenome concept. Birth Defects Res C Embryo Today, 93: 56-66.
• Sharon, G., Segal, D., Ringo, J.M., Hefetz, A., Zilber-Rosenberg I. and Rosenberg E. (2010). Commensal bacteria play a role in mating preference of Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America, 107, 20051–56.
• Shin, S.C., Kim, S.H., You, H., Kim, B., Kim, A.C., Lee, K.A. (2011). Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling. Science, 334, 670–74.
• van den Berg, M. J, Thomas, G., Hendriks, H., van Delden, W. (1984). A reexamination of the negative assortative mating phenomenon and its underlying mechanism in Drosophila melanogaster. Behav Genet 14:45–61.
• Wong, C.N., Ng, P., Douglas, A.E. (2011). Lowdiversity bacterial community in the gut of the fruitfly Drosophila melanogaster. Environmental Microbiology, 13, 1889–1900.
• Woyke, T., Teeling H., Ivanova N.N., Huntemann M., Richter M., Gloeckner F.O., et al. (2006). Symbiosis insights through metagenomic analysis of a microbial consortium. Nature; 443, 950–55.
• Yılmaz, K., and Altındiş, M. (2017). Digestive System Microbiota and Fecal Taransplantation, Nobel Medicus Journal, 13(1). Zhu, X., Han, Y., Du, J., Liu, R., Jin, K., & Yi, W. (2017). Microbiota-gut-brain axis and the central nervous system. Oncotarget, 8(32), 53829