Effects of Perga (Bee bread) on Metamorphosis, Mortality, and Fecundity in Drosophila melanogaster

Yıl 2020, Cilt: 2 Sayı: 2, 1 - 15, 30.08.2020

Mehmet Fidan , Arif Ayar , Vahit Konar

Öz

The present study aimed to investigate the effects of Perga ,whose high biological activity inhibits mold and fungal growth and increases the level of breeding hormones, on the rate of larva-pupa-adult metamorphosis, survival and fecundity in Drosophila melanogaster. The study determined the metamorphosis and mortality rates of 3rd stage D. melanogaster larvae placed in media containing Perga at different concentrations (5, 2.5, 1.25, 0.625, and 0.3125 mg/ml) and in Perga-free media as well as the fecundity rates of female individuals developing from larvae. The obtained data revealed that there was a significant increase in the fecundity rates of female individuals grown in media containing Perga at 5 different concentrations (p <0.05). While the mean fecundity rate was 57±0.88 in female individuals grown in media containing 2.5mg/ml Perga, this rate was found to be 47±1.15 in the standard Drosophila medium containing no Perga, which was the control group. Considering Perga's effects on larval mortality in 3rd stage Drosophila melanogaster larvae, while the percentage of survival was 94% in the control group, it was determined as 98% in the larvae fed with Perga (p <0.05). Considering the metamorphosis rates, no significant increase was observed in the number of individuals who could develop from larva to adult (p> 0.05).
The present study revealed the positive effects of this precious apitherapic product, whose effects and importance are not well known, on vital parameters in D. melanogaster, a eukaryotic model organism.

Anahtar Kelimeler

Bee bread, Perga, Drosophila melanogaster, Fecundity, Mortality, Metamorphosis

Kaynakça

• Albayrak, S. and Albayrak, S. (2008). Propolis: natural antimicrobial agent. Ankara University Faculty of Pharmacy Journal, 37 (3), 201-215.
• Basualdo, M., Barragán, S., & Antúnez, K. (2014). Bee bread increases honeybee haemolymph protein and promote better survival despite of causing higher N osema ceranae abundance in honeybees. Environmental microbiology reports, 6(4), 396-400.
• Bobis, O., Dezmirean, D.S., Marghitas, L.A., Bonta, V., Margaoan, R., Pasca, C., Urcan, A., Bandhari, P.S. (2017). Beebread from Apis mellifera and Apis dorsata. Comparative Chemical Composition and Bioactivity, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Animal Science and Biotechnologies, 74(1), 43-50.
• Bogdanov, S. (2011). Pollen: Nutrition, Functional Properties, Health: A Review. Bee Product Science, pp. 1-34.
• Connor, L.J., Muir, R.G. (2013). Pollen and Bee Bread. Am Bee J,153:727-729.
• Falakalı, B. (1990). Drosophila Genetics, Ege University Printing House, Bornova-İzmir. ss 44.
• Gilliam, M., Prest, D.B. and Lorenz, B.J. (1989). Microbiology of pollen and bee bread: taxonomy and enzymology of moulds. Apidologie 20:53-68.
• Graf, U., Schaik, N. V., Wtirgler F. E. (1992). Drosophila genetics, A Practical Course. Springer-Verlag, Berlin, 239 s.
• Gui, Y., & Grant, A. (2008). Joint effects of density dependence and toxicant exposure on Drosophila melanogaster populations. Ecotoxicology and Environmental Safety, 70(2), 236-243.
• Haydak M.H, (1958). Pollen substitutes.Proc. X International Congres Entomol. Montreal. Vol.4, 1053-1056.
• Hoffman, G.R. (2011). Casarett and Doull’s Toxicology The Basic Science of Poisons, Pergaman Press, New York, 201-217.
• Hoffman, G.D., Eckholm, B.J. and Huang, M.H. (2013). A comparison of bee bread made by Africanized and European honey bees (Apis mellifera) and its effects on hemolymph protein titers. Apidologie 44:52-63.
• Karaman, M.R., Artık, N. and Küçükersan, K. (2016). Perga (Bee Bread) Composition and Health Benefıt. The 2nd International Turkic World Conference on Chemical Sciences and Technologies, Skopje, Macedonia on October 26-30.
• Kowalski, S., & Makarewicz, M. (2017). Functional properties of honey supplemented with bee bread and propolis. Natural product research, 31(22), 2680-2683.
• Krell, R. (1996). Value-added products from beekeeping. FAO Agricultural Services Bulletin No. 124 Food and Agriculture Organization of the United Nations Rome.
• Mutsaers M., Blitterswijk H., Leven L., Kerkvliet J., Waerdt J. (2005). Bee products properties, processing and marketing. Agrodok 42.
• Nagai, T., Nagashima, T., Suzuki, N., Inoue, R. (2005). Antioxidant activity and angiotensin-I converting enzyme inhibition by enzymatic hydolysates from bee bread. Z Naturforsch., 60c, 133-138.
• Lloyd, T. E. and Taylor, J. P. (2010) “Flightless flies: Drosophila models of neuromuscular disease”, Annals of the New York Academy of Sciences, 1184, e1-20.
• Özcan, P. Ö. (2011). Investigation of antimutagenic effects of propolis in Drosophila melanogaster. Master thesis, Hacettepe University Institute of Science, Ankara.
• Özkan, S., & Sancar, K. (2015). Apitherapy and Child Health. Dokuz Eylul University Faculty of Nursing Electronics Journal, 8 (4).
• Pascoal, A., Rodrigues, S., Teixeira, A., Feás, X., & Estevinho, L. M. (2014). Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and anti-inflammatory. Food and Chemical Toxicology, 63, 233-239.
• Reiter, R, (1947). The coloration of anther and corbicular pollen. Ohio J Sci. 1(7), 137-152.
• Uysal, H., Şişman, T ve Aşkın, H. (2006). Drosophila Biology and Crossing Methods (Extended 2nd Edition), Atatürk University Publication No. 941.
• Valadares, B.L.B., Graf, U. Spano, M.A. (2007). Inhibitory effects of water extract of propolis on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster. Food and Chemical Toxicology 46, 1103–1110.
• Vasquez, A., Olofsson, T.C. (2009). The lactic acid bacteria involved in the production of bee pollen and beebread. J Apic Res., 48, 189-195.