Preparation of prebiotic pectin-supplemented vitamin C microcapsules
Yıl 2024,
, 6 - 13, 24.04.2024
Özlem Derya Öztürk
,
Samet Ergün
,
Naciye Özdemir
,
Idris Sargin
,
Gulsin Arslan
Öz
Microencapsulating vitamin C with dietary fibers and prebiotics can improve the storage, preservation, and marketing of vitamin C supplements. This research aimed to explore the feasibility of creating microcapsules using vitamin C, pectin, and alginate through a microencapsulation technique. Pectin was extracted from lemon peel using an acid treatment and then characterised. The morphology of the vitamin C-pectin-alginate microcapsules was examined by scanning electron microscopy. Time, temperature, and pH-dependent vitamin C release profiles of the vitamin C-pectin-alginate microcapsules were studied. The rate of release of vitamin C increased towards pH values close to 7.0, with a higher rate of 83.97% observed at pH 7.0. Additionally, temperature affected the release of vitamin C from the microcapsules, with approximately 47.2% release at body temperature (37°C) and a higher fluctuation in vitamin C release was observed at 20°C. This study revealed that pectin extracted from lemon peels can be used with alginate to encapsulate vitamin C.
Proje Numarası
1919B012215804
Kaynakça
- Adetunji, L. R., Adekunle, A., Orsat, V. and Raghavan, V., 2017, Advances in the pectin production process using novel extraction techniques: A review, Food Hydrocolloids, 62, 239-250.
- Azad, A., Ali, M., Akter, M. S., Rahman, M. J. and Ahmed, M., 2014, Isolation and characterization of pectin extracted from lemon pomace during ripening, Journal of Food and Nutrition Sciences, 2 (2), 30-35.
- Baum, A., Dominiak, M., Vidal-Melgosa, S., Willats, W. G. T., Søndergaard, K. M., Hansen, P. W., Meyer, A. S. and Mikkelsen, J. D., 2017, Prediction of Pectin Yield and Quality by FTIR and Carbohydrate Microarray Analysis, Food and Bioprocess Technology, 10 (1), 143-154.
- Bitton, R., Ben‐Yehuda, M., Davidovich, M., Balazs, Y., Potin, P., Delage, L., Colin, C. and Bianco‐Peled, H., 2006, Structure of algal‐born phenolic polymeric adhesives, Macromolecular bioscience, 6 (9), 737-746.
- Blažević, J., Stanković, A., Šafranko, S., Jokić, S., Velić, D. and Medvidović-Kosanović, M., 2020, ELECTROCHEMICAL DETECTION OF VITAMIN C IN REAL SAMPLES, Hrana u zdravlju i bolesti: znanstveno-stručni časopis za nutricionizam i dijetetiku, 9 (1), 1-8.
- Champagne, C. P. and Fustier, P., 2007, Microencapsulation for the improved delivery of bioactive compounds into foods, Current opinion in biotechnology, 18 (2), 184-190.
- Cho, Y., Shim, H. and Park, J., 2003, Encapsulation of fish oil by an enzymatic gelation process using transglutaminase cross‐linked proteins, Journal of Food Science, 68 (9), 2717-2723.
- Da Silva, J. and Rao, M., 2006, 11 pectins: structure, functionality, and uses, Food polysaccharides and their applications, 353.
- Desai, K. and Park, H. J., 2006, Effect of manufacturing parameters on the characteristics of vitamin C encapsulated tripolyphosphate-chitosan microspheres prepared by spray-drying, Journal of microencapsulation, 23 (1), 91-103.
- Donati, I., Mørch, Y. A., Strand, B. L., Skjåk-Bræk, G. and Paoletti, S., 2009, Effect of elongation of alternating sequences on swelling behavior and large deformation properties of natural alginate gels, The Journal of Physical Chemistry B, 113 (39), 12916-12922.
- Fishman, M., Chau, H., Coffin, D. and Hotchkiss, A., 2003, A comparison of lime and orange pectin which were rapidly extracted from albedo, In: Advances in pectin and pectinase research, Eds: Springer, p. 107-122.
- Fishman, M. L., Chau, H. K., Hoagland, P. and Ayyad, K., 1999, Characterization of pectin, flash-extracted from orange albedo by microwave heating, under pressure, Carbohydrate research, 323 (1-4), 126-138.
- Groudeva, J., Kratchanova, M. G., Panchev, I. N. and Kratchanova, C., 1997, Application of granulated apple pectin in the treatment of hyperlipoproteinaemia I. Deriving the regression equation to describe the changes, Zeitschrift für Lebensmitteluntersuchung und-Forschung A, 204 (5), 374-378.
- Ho, Y.-Y., Lin, C.-M. and Wu, M.-C., 2017, Evaluation of the prebiotic effects of citrus pectin hydrolysate, journal of food and drug analysis, 25 (3), 550-558.
- Islamova, Z. I., Ogai, D., Abramenko, O., Lim, A., Abduazimov, B., Malikova, M. K., Rakhmanberdyeva, R., Khushbaktova, Z. and Syrov, V., 2017, Comparative assessment of the prebiotic activity of some pectin polysaccharides, Pharmaceutical Chemistry Journal, 51 (4), 288-291.
- Ismail, N. S. M., Ramli, N., Hani, N. M. and Meon, Z., 2012, Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions, Sains Malaysiana, 41 (1), 41-45.
- Jang, K.-I. and Lee, H. G., 2008, Stability of chitosan nanoparticles for L-ascorbic acid during heat treatment in aqueous solution, Journal of Agricultural and Food Chemistry, 56 (6), 1936-1941.
- Josef, E., Zilberman, M. and Bianco-Peled, H., 2010, Composite alginate hydrogels: An innovative approach for the controlled release of hydrophobic drugs, Acta Biomaterialia, 6 (12), 4642-4649.
- Jun, H.-I., Lee, C.-H., Song, G.-S. and Kim, Y.-S., 2006, Characterization of the pectic polysaccharides from pumpkin peel, LWT-Food Science and Technology, 39 (5), 554-561.
- Kratchanova, M., Nikolova, M., Pavlova, E., Yanakieva, I. and Kussovski, V., 2010, Composition and properties of biologically active pectic polysaccharides from leek (Allium porrum), Journal of the Science of Food and Agriculture, 90 (12), 2046-2051.
- Li, X., Tang, L., Lin, Y. F. and Xie, G. F., 2018, Role of vitamin C in wound healing after dental implant surgery in patients treated with bone grafts and patients with chronic periodontitis, Clinical Implant Dentistry and Related Research, 20 (5), 793-798.
- Ling, B., Ramaswamy, H. S., Lyng, J. G., Gao, J. and Wang, S., 2022, Roles of physical fields in the extraction of pectin from plant food wastes and byproducts: A systematic review, Food Research International, 112343.
- Maran, J. P., Sivakumar, V., Thirugnanasambandham, K. and Sridhar, R., 2013, Optimization of microwave assisted extraction of pectin from orange peel, Carbohydrate polymers, 97 (2), 703-709.
- Mohamadzadeh, J., Sadeghi-Mahoonak, A., Yaghbani, M. and Aalami, M., 2010, Extraction of pectin from sunflower head residues of selected Iranian cultivars, World Applied Sciences Journal, 8 (1), 21-24.
- Ngai, K. S., Tan, W. T., Zainal, Z., Zawawi, R. M. and Zidan, M., 2013, Voltammetry detection of ascorbic acid at glassy carbon electrode modified by single-walled carbon nanotube/zinc oxide, Int. J. Electrochem. Sci, 8 (8), 10557-10567.
- Njus, D., Kelley, P. M., Tu, Y.-J. and Schlegel, H. B., 2020, Ascorbic acid: The chemistry underlying its antioxidant properties, Free Radical Biology and Medicine, 159, 37-43.
- Özkahraman, B., Torkay, G., Özbaş, Z. and Bal-Öztürk, A., 2023, The effect of vitamin C in the formulation of pectin/thiolated alginate buccal adhesive patches: In vitro and Ex vivo evaluation, International Journal of Adhesion and Adhesives, 120, 103276.
- Peng, X., Yang, G., Shi, Y., Zhou, Y., Zhang, M. and Li, S., 2020, Box–Behnken design based statistical modeling for the extraction and physicochemical properties of pectin from sunflower heads and the comparison with commercial low-methoxyl pectin, Scientific Reports, 10 (1), 3595.
- Pereira, P. H. F., Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Moates, G. K., Wellner, N., Waldron, K. W. and Azeredo, H. M. C., 2016, Pectin extraction from pomegranate peels with citric acid, International Journal of Biological Macromolecules, 88, 373-379.
- Ramachandran, C., Wilk, B. J., Hotchkiss, A., Chau, H., Eliaz, I. and Melnick, S. J., 2011, Activation of human T-helper/inducer cell, T-cytotoxic cell, B-cell, and natural killer (NK)-cells and induction of natural killer cell activity against K562 chronic myeloid leukemia cells with modified citrus pectin, BMC complementary and alternative medicine, 11 (1), 1-9.
- Robyt, J. F., 1998, Essentials of carbohydrate chemistry, Springer Science & Business Media, p.
Said, H. M., 2011, Intestinal absorption of water-soluble vitamins in health and disease, Biochem J, 437 (3), 357-372.
- Santos, E. E., Amaro, R. C., Bustamante, C. C. C., Guerra, M. H. A., Soares, L. C. and Froes, R. E. S., 2020, Extraction of pectin from agroindustrial residue with an ecofriendly solvent: use of FTIR and chemometrics to differentiate pectins according to degree of methyl esterification, Food Hydrocolloids, 107, 105921.
- Schwartz, S. E., Levine, R. A., Weinstock, R. S., Petokas, S., Mills, C. A. and Thomas, F., 1988, Sustained pectin ingestion: effect on gastric emptying and glucose tolerance in non-insulin-dependent diabetic patients, The American journal of clinical nutrition, 48 (6), 1413-1417.
- Sdiri, S., Cuenca, J., Navarro, P., Salvador, A. and Bermejo, A., 2020, New triploids late-maturing mandarins as a rich source of antioxidant compounds, European Food Research and Technology, 246 (1), 225-237.
- Shimoni, E., 2004, Stability and shelf life of bioactive compounds during food processing and storage: soy isoflavones, Journal of Food Science, 69 (6), R160-R166.
- Skrovankova, S., Sumczynski, D., Mlcek, J., Jurikova, T. and Sochor, J., 2015, Bioactive compounds and antioxidant activity in different types of berries, International journal of molecular sciences, 16 (10), 24673-24706.
- Smidsrød, O. and Skja, G., 1990, Alginate as immobilization matrix for cells, Trends in biotechnology, 8, 71-78.
- Steinbüchel, A. and Rhee, S. K., 2005, Polysaccharides and polyamides in the food industry: properties, production, and patents, Wiley-VCH Verlag GmbH & CO. KGaA, p.
- Şen, E., Özdemir, S. and Uğuzdoğan, E., 2021, Meyve kabuğu atıklarından pektin ekstraksiyonu ve karakterizasyonu Extraction and characterization of pectin from waste of fruit peels, Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, 27 (7), 863-872.
- Wang, J. and Friedman, E. A., 1998, Short-chain fatty acids induce cell cycle inhibitors in colonocytes, Gastroenterology, 114 (5), 940-946.
- Yamada, H. and Kiyohara, H., 2007, Immunomodulating activity of plant polysaccharide structures, Comprehensive glycoscience, 4, 663-694.
- Yuswan, M., Al-Obaidi, J. R., Rahayu, A., Sahidan, S., Shazrul, F. and Fauzi, D., 2015, New bioactive molecules with potential antioxidant activity from various extracts of wild edible Gelam mushroom (Boletus spp.), Advances in Bioscience and Biotechnology, 6 (04), 320.
- Zhang, S., Hu, H., Wang, L., Liu, F. and Pan, S., 2018, Preparation and prebiotic potential of pectin oligosaccharides obtained from citrus peel pectin, Food Chemistry, 244, 232-237.
- Zielinski, H., Kozlowska, H. and Lewczuk, B., 2001, Bioactive compounds in the cereal grains before and after hydrothermal processing, Innovative Food Science & Emerging Technologies, 2 (3), 159-169.
Prebiyotik pektin takviyeli C vitamini mikrokapsüllerinin hazırlanması
Yıl 2024,
, 6 - 13, 24.04.2024
Özlem Derya Öztürk
,
Samet Ergün
,
Naciye Özdemir
,
Idris Sargin
,
Gulsin Arslan
Öz
C vitamininin diyet lifleri ve prebiyotiklerle mikrokapsüllenmesi, C vitamini takviyelerinin depolanmasını, korunmasını ve pazarlanmasını iyileştirebilir. Bu çalışmanın amacı, bir mikrokapsülleme tekniği ile C vitamini, pektin ve aljinat kullanarak mikrokapsüller oluşturmanın fizibilitesini araştırmaktır. Pektin, asit muamelesi kullanılarak limon kabuğundan ekstrakte edildi ve karakterize edildi. C vitamini-pektin-aljinat mikrokapsüllerinin morfolojisi taramalı elektron mikroskobu ile incelenmiştir. C vitamini-pektin-aljinat mikrokapsüllerinin zaman, sıcaklık ve pH bağımlı C vitamini salım profilleri incelenmiştir. C vitamini salım hızı, pH 7.0'a yakın pH değerlerine doğru artarken, pH 7.0'da daha yüksek oranda %83.97 gözlendi. Ek olarak sıcaklık, vücut sıcaklığında (37°C) yaklaşık %47.2 salınım ile mikrokapsüllerden C vitamini salınımını etkiledi ve 20°C'de C vitamini salınımında daha yüksek bir dalgalanma gözlendi. Bu çalışma, limon kabuklarından ekstrakte edilen pektinin, C vitaminini kapsüllemek için aljinat ile birlikte kullanılabileceğini ortaya koydu.
Destekleyen Kurum
TÜBİTAK
Proje Numarası
1919B012215804
Teşekkür
The authors thank TUBİTAK for funding the work (2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı, Projet no: 1919B012215804).
Kaynakça
- Adetunji, L. R., Adekunle, A., Orsat, V. and Raghavan, V., 2017, Advances in the pectin production process using novel extraction techniques: A review, Food Hydrocolloids, 62, 239-250.
- Azad, A., Ali, M., Akter, M. S., Rahman, M. J. and Ahmed, M., 2014, Isolation and characterization of pectin extracted from lemon pomace during ripening, Journal of Food and Nutrition Sciences, 2 (2), 30-35.
- Baum, A., Dominiak, M., Vidal-Melgosa, S., Willats, W. G. T., Søndergaard, K. M., Hansen, P. W., Meyer, A. S. and Mikkelsen, J. D., 2017, Prediction of Pectin Yield and Quality by FTIR and Carbohydrate Microarray Analysis, Food and Bioprocess Technology, 10 (1), 143-154.
- Bitton, R., Ben‐Yehuda, M., Davidovich, M., Balazs, Y., Potin, P., Delage, L., Colin, C. and Bianco‐Peled, H., 2006, Structure of algal‐born phenolic polymeric adhesives, Macromolecular bioscience, 6 (9), 737-746.
- Blažević, J., Stanković, A., Šafranko, S., Jokić, S., Velić, D. and Medvidović-Kosanović, M., 2020, ELECTROCHEMICAL DETECTION OF VITAMIN C IN REAL SAMPLES, Hrana u zdravlju i bolesti: znanstveno-stručni časopis za nutricionizam i dijetetiku, 9 (1), 1-8.
- Champagne, C. P. and Fustier, P., 2007, Microencapsulation for the improved delivery of bioactive compounds into foods, Current opinion in biotechnology, 18 (2), 184-190.
- Cho, Y., Shim, H. and Park, J., 2003, Encapsulation of fish oil by an enzymatic gelation process using transglutaminase cross‐linked proteins, Journal of Food Science, 68 (9), 2717-2723.
- Da Silva, J. and Rao, M., 2006, 11 pectins: structure, functionality, and uses, Food polysaccharides and their applications, 353.
- Desai, K. and Park, H. J., 2006, Effect of manufacturing parameters on the characteristics of vitamin C encapsulated tripolyphosphate-chitosan microspheres prepared by spray-drying, Journal of microencapsulation, 23 (1), 91-103.
- Donati, I., Mørch, Y. A., Strand, B. L., Skjåk-Bræk, G. and Paoletti, S., 2009, Effect of elongation of alternating sequences on swelling behavior and large deformation properties of natural alginate gels, The Journal of Physical Chemistry B, 113 (39), 12916-12922.
- Fishman, M., Chau, H., Coffin, D. and Hotchkiss, A., 2003, A comparison of lime and orange pectin which were rapidly extracted from albedo, In: Advances in pectin and pectinase research, Eds: Springer, p. 107-122.
- Fishman, M. L., Chau, H. K., Hoagland, P. and Ayyad, K., 1999, Characterization of pectin, flash-extracted from orange albedo by microwave heating, under pressure, Carbohydrate research, 323 (1-4), 126-138.
- Groudeva, J., Kratchanova, M. G., Panchev, I. N. and Kratchanova, C., 1997, Application of granulated apple pectin in the treatment of hyperlipoproteinaemia I. Deriving the regression equation to describe the changes, Zeitschrift für Lebensmitteluntersuchung und-Forschung A, 204 (5), 374-378.
- Ho, Y.-Y., Lin, C.-M. and Wu, M.-C., 2017, Evaluation of the prebiotic effects of citrus pectin hydrolysate, journal of food and drug analysis, 25 (3), 550-558.
- Islamova, Z. I., Ogai, D., Abramenko, O., Lim, A., Abduazimov, B., Malikova, M. K., Rakhmanberdyeva, R., Khushbaktova, Z. and Syrov, V., 2017, Comparative assessment of the prebiotic activity of some pectin polysaccharides, Pharmaceutical Chemistry Journal, 51 (4), 288-291.
- Ismail, N. S. M., Ramli, N., Hani, N. M. and Meon, Z., 2012, Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions, Sains Malaysiana, 41 (1), 41-45.
- Jang, K.-I. and Lee, H. G., 2008, Stability of chitosan nanoparticles for L-ascorbic acid during heat treatment in aqueous solution, Journal of Agricultural and Food Chemistry, 56 (6), 1936-1941.
- Josef, E., Zilberman, M. and Bianco-Peled, H., 2010, Composite alginate hydrogels: An innovative approach for the controlled release of hydrophobic drugs, Acta Biomaterialia, 6 (12), 4642-4649.
- Jun, H.-I., Lee, C.-H., Song, G.-S. and Kim, Y.-S., 2006, Characterization of the pectic polysaccharides from pumpkin peel, LWT-Food Science and Technology, 39 (5), 554-561.
- Kratchanova, M., Nikolova, M., Pavlova, E., Yanakieva, I. and Kussovski, V., 2010, Composition and properties of biologically active pectic polysaccharides from leek (Allium porrum), Journal of the Science of Food and Agriculture, 90 (12), 2046-2051.
- Li, X., Tang, L., Lin, Y. F. and Xie, G. F., 2018, Role of vitamin C in wound healing after dental implant surgery in patients treated with bone grafts and patients with chronic periodontitis, Clinical Implant Dentistry and Related Research, 20 (5), 793-798.
- Ling, B., Ramaswamy, H. S., Lyng, J. G., Gao, J. and Wang, S., 2022, Roles of physical fields in the extraction of pectin from plant food wastes and byproducts: A systematic review, Food Research International, 112343.
- Maran, J. P., Sivakumar, V., Thirugnanasambandham, K. and Sridhar, R., 2013, Optimization of microwave assisted extraction of pectin from orange peel, Carbohydrate polymers, 97 (2), 703-709.
- Mohamadzadeh, J., Sadeghi-Mahoonak, A., Yaghbani, M. and Aalami, M., 2010, Extraction of pectin from sunflower head residues of selected Iranian cultivars, World Applied Sciences Journal, 8 (1), 21-24.
- Ngai, K. S., Tan, W. T., Zainal, Z., Zawawi, R. M. and Zidan, M., 2013, Voltammetry detection of ascorbic acid at glassy carbon electrode modified by single-walled carbon nanotube/zinc oxide, Int. J. Electrochem. Sci, 8 (8), 10557-10567.
- Njus, D., Kelley, P. M., Tu, Y.-J. and Schlegel, H. B., 2020, Ascorbic acid: The chemistry underlying its antioxidant properties, Free Radical Biology and Medicine, 159, 37-43.
- Özkahraman, B., Torkay, G., Özbaş, Z. and Bal-Öztürk, A., 2023, The effect of vitamin C in the formulation of pectin/thiolated alginate buccal adhesive patches: In vitro and Ex vivo evaluation, International Journal of Adhesion and Adhesives, 120, 103276.
- Peng, X., Yang, G., Shi, Y., Zhou, Y., Zhang, M. and Li, S., 2020, Box–Behnken design based statistical modeling for the extraction and physicochemical properties of pectin from sunflower heads and the comparison with commercial low-methoxyl pectin, Scientific Reports, 10 (1), 3595.
- Pereira, P. H. F., Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Moates, G. K., Wellner, N., Waldron, K. W. and Azeredo, H. M. C., 2016, Pectin extraction from pomegranate peels with citric acid, International Journal of Biological Macromolecules, 88, 373-379.
- Ramachandran, C., Wilk, B. J., Hotchkiss, A., Chau, H., Eliaz, I. and Melnick, S. J., 2011, Activation of human T-helper/inducer cell, T-cytotoxic cell, B-cell, and natural killer (NK)-cells and induction of natural killer cell activity against K562 chronic myeloid leukemia cells with modified citrus pectin, BMC complementary and alternative medicine, 11 (1), 1-9.
- Robyt, J. F., 1998, Essentials of carbohydrate chemistry, Springer Science & Business Media, p.
Said, H. M., 2011, Intestinal absorption of water-soluble vitamins in health and disease, Biochem J, 437 (3), 357-372.
- Santos, E. E., Amaro, R. C., Bustamante, C. C. C., Guerra, M. H. A., Soares, L. C. and Froes, R. E. S., 2020, Extraction of pectin from agroindustrial residue with an ecofriendly solvent: use of FTIR and chemometrics to differentiate pectins according to degree of methyl esterification, Food Hydrocolloids, 107, 105921.
- Schwartz, S. E., Levine, R. A., Weinstock, R. S., Petokas, S., Mills, C. A. and Thomas, F., 1988, Sustained pectin ingestion: effect on gastric emptying and glucose tolerance in non-insulin-dependent diabetic patients, The American journal of clinical nutrition, 48 (6), 1413-1417.
- Sdiri, S., Cuenca, J., Navarro, P., Salvador, A. and Bermejo, A., 2020, New triploids late-maturing mandarins as a rich source of antioxidant compounds, European Food Research and Technology, 246 (1), 225-237.
- Shimoni, E., 2004, Stability and shelf life of bioactive compounds during food processing and storage: soy isoflavones, Journal of Food Science, 69 (6), R160-R166.
- Skrovankova, S., Sumczynski, D., Mlcek, J., Jurikova, T. and Sochor, J., 2015, Bioactive compounds and antioxidant activity in different types of berries, International journal of molecular sciences, 16 (10), 24673-24706.
- Smidsrød, O. and Skja, G., 1990, Alginate as immobilization matrix for cells, Trends in biotechnology, 8, 71-78.
- Steinbüchel, A. and Rhee, S. K., 2005, Polysaccharides and polyamides in the food industry: properties, production, and patents, Wiley-VCH Verlag GmbH & CO. KGaA, p.
- Şen, E., Özdemir, S. and Uğuzdoğan, E., 2021, Meyve kabuğu atıklarından pektin ekstraksiyonu ve karakterizasyonu Extraction and characterization of pectin from waste of fruit peels, Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, 27 (7), 863-872.
- Wang, J. and Friedman, E. A., 1998, Short-chain fatty acids induce cell cycle inhibitors in colonocytes, Gastroenterology, 114 (5), 940-946.
- Yamada, H. and Kiyohara, H., 2007, Immunomodulating activity of plant polysaccharide structures, Comprehensive glycoscience, 4, 663-694.
- Yuswan, M., Al-Obaidi, J. R., Rahayu, A., Sahidan, S., Shazrul, F. and Fauzi, D., 2015, New bioactive molecules with potential antioxidant activity from various extracts of wild edible Gelam mushroom (Boletus spp.), Advances in Bioscience and Biotechnology, 6 (04), 320.
- Zhang, S., Hu, H., Wang, L., Liu, F. and Pan, S., 2018, Preparation and prebiotic potential of pectin oligosaccharides obtained from citrus peel pectin, Food Chemistry, 244, 232-237.
- Zielinski, H., Kozlowska, H. and Lewczuk, B., 2001, Bioactive compounds in the cereal grains before and after hydrothermal processing, Innovative Food Science & Emerging Technologies, 2 (3), 159-169.