Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu

Yıl 2017, Cilt: 43 Sayı: 2, 175 - 188, 31.10.2017

Sengul Uysal Gokhan Zengin Gokalp Ozmen Guler Abdurrahman Aktumsek

Öz

Birçok çalışma oksidatif stresle ilişkili çeşitli
hastalıkların engellenmesinin yanı sıra insan sağlığına bitki sekonder
metabolitlerinden kaynaklanan faydaları göstermiştir. Bu çalışmada Seseli tortuosum’un antioksidan
aktivitesi serbest radikal süpürme (DPPH), indirgeme gücü (FRAP ve CUPRAC),
toplam antioksidan kapasite (β-karoten/linoleik asit ve fosfomolibdat test)
içeren farklı kimyasal metotlar tarafından araştırıldı. Ayrıca, toplam fenolik
içerik Folin-Ciocalteu testi tarafından tespit edildi. BHA, BHT ve Troloks bu
antioksidan testlerde standart olarak kullanıldı. S. tortuosum’un yağ asiti kompozisyonu Gaz Kromatografisi (GC)
tarafından değerlendirildi. Özütte toplam fenolik içerik 36.58 mgGAE/g özüt
olarak tespit edildi. Özüt antioksidan deneylerde konsantrasyona bağlı olarak
antioksidan aktiviteye sahiptir. S.
tortuosum yağında 24 yağ asiti tanımlandı. Yağ asitleri arasında, linoleik
asit C 18:2 ω6 (%34.57) ana bileşendir. Doymamış yağ asitlerinin miktarı doymuş
yağ asitlerinden daha yüksektir. Bu sonuçlardan,  S.
tortuosum yeni gıda ve ilaç ürünlerinin tasarlanması için değerli bir kaynak
olarak düşünülebilir.

Anahtar Kelimeler

Antioksidan aktivite, yağ asidi bileşimi, fenolik içerik, Seseli tortuosum

Antioxidant Activity and Fatty Acid Composition of Seseli tortuosum

Öz

 Many
studies have shown the benefits deriving from plants secondary metabolites to
human health also for the prevention of several diseases associated to
oxidative stress. In this work, the antioxidant activity of Seseli tortuosum was investigated by
different chemical methods including free radical scavenging (DPPH assay),
reducing power (FRAP and CUPRAC assays), total antioxidant capacity
(β-caroten/linoleic acid and phosphomolybdate assays). Also, total phenolic
content were detected by Folin-Ciocalteu assay. BHA, BHT and Trolox were used
as standards in these antioxidant assays. The fatty acid composition of S. tortuosum was also evaluated by gas
chromatography (GC). Total phenolic content in the extract was detected as
36.58 mgGAE/g extract. The extract possesses antioxidant activity in a
concentration-dependent manner at the antioxidant assays. Twenty-four fatty
acids were identified in the S. tortuosum
oil. Among fatty acids, the main component was linoleic acid (C 18:2 ω6)
(34.57%). The content in unsaturated fatty acids (UFAs) was higher than that of
saturated fatty acids (SFAs). From these results, S. tortuosum could be considered as a valuable source for designing
novel food and pharmaceutical products.

Anahtar Kelimeler

Antioxidant activity, fatty acid composition, phenolic content, Seseli tortuosum

Kaynakça

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