Molecular characterization of Beet necrotic yellow vein virus isolates from sugar beet production areas of Türkiye according to coat protein gene

Öz

Beet necrotic yellow vein virus (BNYVV), which is causes the disease known as 'rhizomania' and is transmitted by the soil-borne vector Polymyxa betae, is the most destructive and prevelant soil-borne virus in sugar beet production areas. In this study, 17 soil samples known to be infested with BNYVV were collected from different regions of Türkiye. Initially, BNYVV isolates were propagated using the bait plant test method. The coat protein (CP) gene is synthesized from the RNA-2 segment of BNYVV. In this study, nucleotide sequences of the CP gene region were obtained from 16 BNYVV isolates. BLAST analysis and phylogenetic tree comparisons with global isolates revealed that BNYVV isolates from Türkiye are located into two distinct groups. In the first group, IGR-9 and ERC-52 isolates exhibited an A (P) type CP structure, showing the highest similarity (99.65%) with HXL291CP-1 (China), Pithiviers (France), and IR-GR1 (Iran), respectively. Meanwhile, the remaining 14 BNYVV isolates in the second group showed the highest similarity (99.65%-100%) to A-type CP-containing isolates from Spain, the USA, and Serbia.

Anahtar Kelimeler

• Rhizomania
• BNYVV
• CP
• RT-PCR
• sequencing

Türkiye şeker pancarı üretim alanlarına ait Beet necrotic yellow vein virus izolatlarının kılıf protein genine göre moleküler karakterizasyonu

Öz

Şeker pancarı üretim alanlarında sıklıkla rastlanan, en fazla zarar oluşturan toprak kökenli virüs türü Beet necrotic yellow vein virus (BNYVV) olup 'rhizomania' olarak bilinen hastalığa neden olmakta ve toprak kökenli vektör Polymyxa betae ile taşınmaktadır. Bu çalışmada, Türkiye’nin farklı bölgelerinden elde edilen ve BNYVV ile bulaşık olduğu daha önce belirlenen 17 toprak örneği kullanılmıştır. Öncelikle, tuzak bitki testi yöntemine göre BNYVV izolatlarının yeniden çoğaltımı sağlanmıştır. Kılıf protein (CP) geni BNYVV’nin RNA-2 segmentinden sentezlenmektedir. Bu çalışmada 16 BNYVV izolatının CP gen bölgesinin nükleotit dizileri elde edilmiştir. Bu izolatların dünya izolatları ile BLAST analizi ve elde edilen filogenetik analizler sonucu, ülkemize ait BNYVV izolatlarının iki farklı gruba ayrıldıkları saptanmıştır. İlk grupta yer alan IGR-9 ve ERC-52 izolatlarının, A (P) tipi CP yapısına sahip Çin, Fransa ve İran’a ait bahsedildiği sıraya göre, HXL291CP-1, Pithiviers ve IR-GR1 izolatlarıyla %99.65 oran ile en yüksek benzerliğe sahip olduğu belirlenmiştir. İkinci grupta yer alan diğer 14 BNYVV izolatı ise, A-tip karakterde CP içeren İspanya, ABD ve Sırbistan’a ait BNYVV izolatları ile en yüksek benzerlik göstermişlerdir (%99.65-%100).

Anahtar Kelimeler

• Rhizomania
• CP
• RT-PCR
• sekans analizi

Kaynakça

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