Isolation and identification of Streptomyces species isolated from forest area (Sakarya City) and determination the production of some extracellular enzyme

Öz

In this study, forest area located in the province of Sakarya city soil bacteria of the genus Streptomyces isolated and made computer-assisted diagnosis and various enzyme activities of the isolates were determined. All isolates of phenotypic and biochemical characteristics of the data being transmitted to the computer program determined IDENTAX, diagnosis is made by determining their position in the genus. Isolates in the color group analysis based on aerial and substrate mycelium color is divided into four main groups. Diagnostic test results of Streptomyces isolates are completed by a computer program MVSP 3.2and were created similarity dendograms. In addition, all isolates protease, amylase, lipase, cellulase and asparaginase enzyme activity was determined.

Anahtar Kelimeler

• Streptomyces,Extracellular enzyme,characterization

Sakarya ili ormanlık alan topraklarından Streptomyces türlerinin izolasyonu, karakterizasyonu ve bazı ekstraselüler enzimlerin üretiminin belirlenmesi

Öz

Bu çalışmada, Sakarya ili sınırlarında bulunan ormanlık alan topraklarından Streptomyces cinsi bakterileri izole edilerek bilgisayar yardımıyla teşhisleri yapılmış ve izolatların amilaz, selülaz, L-asparaginaz, proteaz ve lipaz enzimlerinin aktiviteleri belirlenmiştir. Yapılan renk grubu analizinde izolatlar havasal ve misel rengi esas alınarak 4 ana gruba ayrılmıştır. Teşhisleri tamamlanan Streptomyces izolatlarının test sonuçları MVSP 3.2 bilgisayar programı ile birbirleri arasındaki benzerlik dendogramları oluşturulmuştur. Tüm izolatların fenotipik ve biyokimyasal karakterleri belirlenerek IDENTAX bilgisayar programına bu veriler aktarılmış, cins içerisindeki pozisyonları belirlenerek teşhisleri yapılmıştır. Bu teşhislere göre 80 izolattan 49’unun Streptomyces exfoliatus, 14’ünün Streptomyces atroolivaceus, 1’i Streptomyces albidoflavus,1’i Streptomyces chromofuscus, 1’i de Streptomyces purpureus olduğu belirlenmiştir. Ayrıca tüm izolatların proteaz, amilaz, lipaz, selülaz ve asparaginaz enzim aktiviteleri belirlenmiştir.

Anahtar Kelimeler

• Streptomyces,ekstraselüler enzim,karakterizasyon

Kaynakça

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