Biological control of grapewine crown gall disease agent Agrobacterium vitis by using plant growth promoting rhizobacteria

Yıl 2012, Cilt: 3 Sayı: 1, 21 - 36, 13.07.2016

Mustafa Küsek Özden Çınar

Öz

Crown gall, caused by the bacterium Agrobacterium vitis, is a common and devastating grape disease in many vineyards of the world, including in Turkey. In this study, possibility of biological control of grapewine crown gall disease was investigated. Totally 464 putative Plant Growth Promoting Rhizobacteria (PGPR) were isolated from 39 soil samples which were collected from vineyards of Mersin, Adana, Osmaniye, Hatay, Gaziantep and Kahramanmaraş provinces. Sixty-three bacterial strains solubilized phosphorus on solid NBRIP medium. Ten PGPR strains which have solubilized phosphorus with the highest ratio were used in pot experiments. PGPR strains, Ga7/3-6 (45%), Ga10/2- 5 (97%), Os1/3-1 (80%), OsD1/3-1 (98%) ve HaD6/3-1 (75%), significantly reduced galls weight on grape plants in pot experiments. This is the first study on biological control of Agrobacterium vitis in Turkey.

Anahtar Kelimeler

Biological control, grapevine, gall, Agrobacterium vitis, PGPR

Bitki büyümesini teşvik eden kök bakterilerini kullanılarak asma uru hastalığı etmeni Agrobacterium vitis ’in biyolojik mücadelesi

Öz

Agrobacterium vitisin neden olduğu ur hastalığı, Türkiyeyi de içine alan dünyanın asma yetiştirilen pek çok yerinde yaygın ve yıkıcı bir hastalıktır. Bu çalışmada asma uru hastalığının biyolojik mücadele olanakları araştırılmıştır. Mersin, Adana, Osmaniye, Hatay, Gaziantep ve Kahramanmaraş illerinde bulunan bağ alanlarından bitki büyümesini teşvik eden aday kökbakterisi (PGPR) izolatları elde etmek için 39 toprak örneği alınmış olup toprak örneklerinden 464 adet bakteri izole edilmiştir. Katı NBRIP besi yeri kullanılarak bu bakteri izolatlarından 63’ünün fosforu çözdüğü belirlenmiştir. En yüksek fosfor çözen 10 izolat seçilerek saksı çalışmalarında kullanılmıştır. Saksı çalışmasında PGPR izolatları olan Ga7/3-6 (% 45), Ga10/2-5 (% 97), Os1/3-1 (% 80), OsD1/3-1 (% 98) ve HaD6/3-1 (% 75)’nın asmada ur ağırlığını önemli düzeyde azalttığı belirlenmiştir. Bu çalışma, Türkiye’de Agrobacterium vitis’in PGPR ile biyolojik mücadelesi üzerine ilk çalışma niteliğindedir.

Anahtar Kelimeler

Biyolojik mücadele, asma, ur, Agrobacterium vitis, PGPR

Kaynakça

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