Yıl 2017, Cilt 8 , Sayı 2, Sayfalar 107 - 124 2017-12-15

Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması
Use of Endophytic Bacteria for the Biological Control of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Pınar ÖZSARI [1] , Mustafa AKBABA [2] , Hatice ÖZAKTAN [3] , Yusuf KARSAVURAN [4]


Bu çalışmanın amacı; laboratuvar stoklarından seçilen 19 endofitik bakteri (EB) izolatının ve daha önce renk değişimi gözlenen Patates böceği larvasından izole edilen bir Pseudomonas fluorescens 184 (Pf) izolatının L. decemlineata larvalarına etkilerinin araştırılmasıdır. Birinci, ikinci, üçüncü ve dördüncü dönem L. decemlineata larvaları 16 saat süreyle aç bırakıldıktan sonra, EB süspansiyonu uygulanmış sağlıklı patates yaprakları bulunan petri kaplarına bırakılmıştır. Negatif kontrol olarak sadece steril su ile uygulama görmüş larva ve patates yaprakları kullanılmıştır. Petri kaplarında 8 gün süreyle tutulan larvaların ortalama ölüm oranı (%) saptanmıştır. Testlenen EB izolatlarından Pantoea agglomerans (Pa) CC372-83larvalarda %80 ölüm oranıyla en başarılı uygulama olmuştur. L. decemlineata larvalarının biyolojik mücadelesi açısından koparılmış yaprak testinin duyarlı ve hızlı bir ön eleme yöntemi olduğu dikkati çekmiştir. Bu çalışma in vivo saksı denemeleri ile devam edecektir.   
 

Abstract: The main purpose of this study was to investigate the biocontrol potential of 19 strains of endophytic bacteria (EB), and Pseudomonas fluorescens 184 (Pf) which was isolated from diseased Leptinotarsa decemlineata, on larvae of L. decemlineata. First, second, third and fourth-instar larvae were starved for 16-h. They were then placed in petri dishes containing fresh potato leaves and sprayed with the EB suspensions and P. fluorescens 184. Larvae and potato leaves treated with sterile distilled water was used the negative control. The larvae were incubated for 8 days to determine the mean mortality percentage. The highest insecticidal activity, 80% mortality within 8 days, was in larvae treated with EB strain Pantoea agglomerans CC372-83. This test on fresh potato leaves was a quick and reliable screening method for potential biological control agents of L. decemlineata larvae. This work will continue with in vivo pot test.

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Birincil Dil tr
Konular Mühendislik, Ziraat
Bölüm Makaleler
Yazarlar

Yazar: Pınar ÖZSARI

Yazar: Mustafa AKBABA

Yazar: Hatice ÖZAKTAN

Yazar: Yusuf KARSAVURAN

Tarihler

Yayımlanma Tarihi : 15 Aralık 2017

Bibtex @araştırma makalesi { tbmd401313, journal = {Türkiye Biyolojik Mücadele Dergisi}, issn = {2146-0035}, eissn = {2548-1002}, address = {Çukurova Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü 01330 Sarıçam(Balcalı)-Adana/Türkiye}, publisher = {Türkiye Biyolojik Mücadele Derneği}, year = {2017}, volume = {8}, pages = {107 - 124}, doi = {}, title = {Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması}, key = {cite}, author = {ÖZSARI, Pınar and AKBABA, Mustafa and ÖZAKTAN, Hatice and KARSAVURAN, Yusuf} }
APA ÖZSARI, P , AKBABA, M , ÖZAKTAN, H , KARSAVURAN, Y . (2017). Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması. Türkiye Biyolojik Mücadele Dergisi , 8 (2) , 107-124 . Retrieved from https://dergipark.org.tr/tr/pub/tbmd/issue/35810/401313
MLA ÖZSARI, P , AKBABA, M , ÖZAKTAN, H , KARSAVURAN, Y . "Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması". Türkiye Biyolojik Mücadele Dergisi 8 (2017 ): 107-124 <https://dergipark.org.tr/tr/pub/tbmd/issue/35810/401313>
Chicago ÖZSARI, P , AKBABA, M , ÖZAKTAN, H , KARSAVURAN, Y . "Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması". Türkiye Biyolojik Mücadele Dergisi 8 (2017 ): 107-124
RIS TY - JOUR T1 - Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması AU - Pınar ÖZSARI , Mustafa AKBABA , Hatice ÖZAKTAN , Yusuf KARSAVURAN Y1 - 2017 PY - 2017 N1 - DO - T2 - Türkiye Biyolojik Mücadele Dergisi JF - Journal JO - JOR SP - 107 EP - 124 VL - 8 IS - 2 SN - 2146-0035-2548-1002 M3 - UR - Y2 - 2019 ER -
EndNote %0 Türkiye Biyolojik Mücadele Dergisi Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması %A Pınar ÖZSARI , Mustafa AKBABA , Hatice ÖZAKTAN , Yusuf KARSAVURAN %T Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması %D 2017 %J Türkiye Biyolojik Mücadele Dergisi %P 2146-0035-2548-1002 %V 8 %N 2 %R %U
ISNAD ÖZSARI, Pınar , AKBABA, Mustafa , ÖZAKTAN, Hatice , KARSAVURAN, Yusuf . "Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması". Türkiye Biyolojik Mücadele Dergisi 8 / 2 (Aralık 2017): 107-124 .
AMA ÖZSARI P , AKBABA M , ÖZAKTAN H , KARSAVURAN Y . Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması. Türk. biyo. müc. derg. 2017; 8(2): 107-124.
Vancouver ÖZSARI P , AKBABA M , ÖZAKTAN H , KARSAVURAN Y . Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması. Türkiye Biyolojik Mücadele Dergisi. 2017; 8(2): 124-107.