Yaygın olarak kullanılan bazı biyopestisitlerin yerel entomopatojen nematod izolatlarının hayatta kalma ve virülensliği üzerindeki etkileri

Yıl 2020, Cilt: 11 Sayı: 1, 35 - 41, 30.06.2020

Ebubekir Yüksel , Ramazan Canhılal

https://doi.org/10.31019/tbmd.620137

Öz

Bazı yerel entomopatojen nematod (EPN)
izolatlarının [Heterorhabditis
bacteriophora FLH-4H  (Poinar, 1975)
(Rhabditida: Heterorhabditidae), Steinernema
carpocapsae KCS-4S (Weiser, 1955)
(Rhabditida: Steinernematidae) ve Steinernema
feltiae KMP-9S (Filipjev, 1934)
(Rhabditida: Steinernematidae)] bazı biyopestisitlerle (40 g/ L Azadirachtin,
480 g/ L Spinosad) olan uyumluluğu laboratuvar şartları altında incelenmiştir.
Bu biyopestisitlerin, EPN izolatlarına ait infektiv juvenillerin (IJ) canlılığı
ve virülensliği üzerindeki etkisi, IJ’leri biyopestisitlerin önerilen en yüksek
arazi konsantrasyonlarına 24 ve 48 saat boyunca maruz bırakılarak
incelenmiştir. Biyopestisitlere 24 ve 48 saatlik sürelerde maruz kalan EPN
izolatlarının hayatta kalma oranları % 5-21 arasında değişmiştir. Heterorhabditis bacteriophora FLH-4H
izolatına ait IJ’lerin ölüm oranı her iki uygulama süresinde de %12’yi
geçmemiştir ve test edilen biyopestisitlere en tolerant izolat olduğu
belirlenmiştir. Test edilen biyopestisitler, EPN'ların virülensliğini önemli
düzeyde etkilememiştir ve Galleria mellonella L. (Lepidoptera: Pyralidae) larvalarının
ölüm oranları % 70-95 arasında değişmiştir.

Anahtar Kelimeler

Entomopatojen nematod, Steinernema sp., Heterorhabditis sp., Azadirachtin, Spinosad

Effects of some commonly used biopesticides on the survival and virulence of native entomopathogenic nematode isolates

Öz

The compatibility of the
use of native Turkish entomopathogenic nematode (EPN) isolates, Heterorhabditis bacteriophora FLH-4H
(Poinar, 1975) (Rhabditida: Heterorhabditidae), Steinernema carpocapsae KCS-4S (Weiser, 1955) (Rhabditida:
Steinernematidae) and Steinernema feltiae
KMP-9S (Filipjev, 1934), with two biopesticides, 40 g/ L Azadirachtin and 480
g/ L Spinosad, was investigated under laboratory conditions. The effects of
these biopesticides on the viability and virulence of IJs of EPN isolates was
studied by direct exposure to the biopesticides for 24 h and 48 h at the
highest concentrations recommended for field application. Survival rates of the
EPN isolates ranged between 5% and 21%. Heterorhabditis
bacteriophora FLH-4H was the most
tolerant isolate, with mortality of IJs not exceeding 12% for both exposure
times. The biopesticides did not affect the virulence of the EPNs, with the
mortality of Galleria mellonella L.
(Lepidoptera: Pyralidae) larvae ranging between 70% and 95%.

Anahtar Kelimeler

Entomopathogenic nematodes, Steinernema sp., Heterorhabditis, Azadirachtin, Spinosad

Kaynakça

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