Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat

Abstract

This manuscript has aimed to investigate the nephro-hepato protective potential and antioxidant role of lyophilized extract of hawthorn (Crataegus orientalis L) fruit (LEH) against carbon tetrachloride (CCl4) toxicity. The experimental were conducted as; 0.5 ml/kg CCl4, bw), 100 mg/kg LEH bw, 200 mg/kg LEH bw, CCl4 0.5 ml/kg + 100 mg/kg LEH bw and CCl4 0.5 ml/kg + 200 mg/kg LEH bw treated with the extract for 3 weeks. At the end of the experimental treatment, the nephro-hepato protective potential and antioxidant capacity of the LEH was evaluated measuring by liver and kidney damage serum biomarkers, antioxidant defense systems constituents (ADSCs) and malondialdehyde (MDA) content in the erythrocyte, brain, kidney and liver tissues of rats. According to results; liver damage serum enzymes activities and MDA content of the tissues were significantly higher in CCl4 group compared to normal control (NC) whereas; these parameters were significantly lower in extract supplemented groups compared to CCl4 group. According to the resuls, the study results suggest that the LEH supplementations diet restored most of the parameters towards the NC values with fluctuations in the ADSCs. Therefore, it is thought that the extract of hawthorn has antioxidant capacity and hepatoprotective effects against in CCl4-intoxicated rats.

Keywords

• Carbon tetrachloride
• Hawthorn Fruit
• Protective and Antioxidant role

Alıç Meyvesi Liyofilize Ekstraktının Sıçanlarda Karbon Tetraklorür Toksisitesine Karşı Antioksidan ve Koruyucu Rolünün Değerlendirilmesi

Öz

Bu makale, karbon tetraklorür (CCl4) toksisitesine karşı alıç (Crataegus orientalis L.) meyve liyofilize ekstrakt (LEH), böbrek ve karaciğer koruyucu potansiyelini ve antioksidan rolünü araştırmayı amaçlamaktadır. Deneysel dizayn; Normal kontrol (NK) CCl4 grubu (0,5 ml / kg CCl4, vücut ağırlığı (wb)), LEH1grubu (100 mg / kg LEH bw), LEH4 grubu (200 mg / kg LEH bw), CCl4+LEH1 grubu (0,5 ml / kg + 100 mg / kg LEH bw) ve CCl4+LEH4 grubu (0,5 ml / kg + 200 mg / kg LEH bw) şeklinde 3 haftalık ekstre muamelesi ile yapılmıştır. Deneysel muamelenin sonunda: LEH'nin böbrek ve karaciğer koruyucu potansiyeli ve antioksidan rolünü, karaciğer ve böbrek hasarı serum biyobelirteçleri, eritrosit, beyin, börek ve karaciğerdeki antioksidan savunma sistemleri bileşenleri (ASSB) ile malondialdehit (MDA) içeriği ölçülerek değerlendirildi. Sonuçlara göre; karaciğer hasarı serum enzim aktiviteleri ve dokuların MDA içeriği CCl4 grubunda NK göre anlamlı olarak daha yüksekti. Bu parametreler, ekstre takviyeli gruplarda CCl4 grubuna kıyasla önemli ölçüde daha düşüktü. Sonuçlara göre, LEH takviyeli diyetinin ASSB'lerdeki dalgalanmaları NK değerlerine doğru parametrelerin çoğunu geri çektiğini göstermektedir. Bu nedenle, CCl4 ile toksisite oluşturulan sıçanlarda alıç bitkisi meyve ekstresinin antioksidan kapasiteye ve karaciğer koruyucu etkilere sahip olabileceği düşünülmektedir.

Anahtar Kelimeler

• Karbon tetraklorür
• Alıç meyvesi
• Koruyucu ve antioksidan rolü

Kaynakça

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